LOCUS       sequence-354941-       10789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pacr-5::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression
            of UNC-7-wCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Kawano T, Po MD, Gao S, Leung G, Ryu WS, Zhen M
  TITLE     An imbalancing act: gap junctions reduce the backward motor circuit 
            activity to bias C. elegans for forward locomotion.
  JOURNAL   Neuron. 2011 Nov 17;72(4):572-86. doi: 10.1016/j.neuron.2011.09.005.
  PUBMED    22099460
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2011.09"; journalName: "Neuron"; date: 
            "2011-11-17- 17"; volume: "72"; issue: "4"; pages: "572-86"
COMMENT     Sequence Label: pJH2164
FEATURES             Location/Qualifiers
     source          1..10789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(141..146,147..164,165..171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     184..206
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     203..219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     203..219
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(5914..5925)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     complement(8577..8599)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8699..8718
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8796..8814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8882..8986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8987..9055,9056..9847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9205..9224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10018..10606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10507..10526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10760..10777
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
       61 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      121 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      181 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa
      241 gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg
      301 ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgggctg
      361 cagcatgcgg ccgcatttgt tgaaaaaacg tacggtcttc atttaagagt gacatagttt
      421 cttcttacat ttttaatatt aatctgatgt ttcagatctg ctctaataat ggctggttca
      481 gcacatcaaa acgagtacga cttttcgcaa cgacggcatt cacttatcaa catgttcaag
      541 gcgtttaaat tgtaaacaat cttgtgatgc agcattgctc caatgataat ttgggtattt
      601 catagggtgt tattttgcat ctgtttatct atgaagaagc tgaaaactgg aattatcatt
      661 agagcaagtt cgcaagcaga ccagcacaca aaataatgca acagaatttg gttcccaaag
      721 agctcaataa agtttacttt tcacaaatgc ttcaatttcc gtttcttcca tttaaattgt
      781 tctgatgtta tagcttctcg cactttccct ccatttcaat ttcccgccta ttttctaccc
      841 tcaaaagttc ctctcatagt tttcttattg atgaggaact tttgggcatt agcaaaattg
      901 tgccctttga attaagttaa aatgaattaa ataatctttt attaaagcga attcgcatca
      961 tattttaaat gcgcggtatt aataaaaata ggttcgtcga tcaataggtg ttttcatatt
     1021 atccaaatac acaattgggc aatttgtaaa acttgaagaa tgcggcaacg agttctgtgc
     1081 aagtctttac ctttaatgct tccgtcatcg ttagcaaaaa ctgtgtcatg aaagacaagt
     1141 gtataagatt attcaaatac aattttctta tggagttttt ctagctttta aggatttcca
     1201 aatagaatga tcattccttg aataataatt ctctatttta tcaatatttt gttttttttt
     1261 ctattttaga aagggcggag agtagtgtgt agtggcggtt tcacgctctg atgatctcat
     1321 gccattgttc accttttcca aattgtttta ggttattcgg agtacttctt ttctttcttt
     1381 tccctcttca ttttcaccct ctgtttgagc atgagtgctc tctattcttt ggtgatggaa
     1441 taaaaagagg tgccatcaaa ttcagaattt tgtaaaatgt tgaactttag agtttttagt
     1501 attcggttag tgtgatgttt gttttatttt caaccctaac aaatctcagt tttctggaaa
     1561 agttgtgcat ttacaatgaa tatattgttt tctgcatttc gtctgttctt agattattca
     1621 gtgactttgg aaatttaagc taattcttgt tgagccattt gacataattt gtatagaaaa
     1681 caaaaatgta tactttacac cgcgatttag atacggcact gtgcggaaac tagcaaacaa
     1741 ttcagagccg acttccataa ggttgccaaa aaaattttag taatttatta attcaataat
     1801 taaatgtatt tatcacgaaa ataaaatgaa aacatttgtt gttcaaaatt atatattttc
     1861 agaaagaaaa aacaagtttt taaaatttaa ttactacttc tcattatgag tctctcaaaa
     1921 agctcaacat ttcagttaga ataaaaatta aaaaatatca atgaaagtgt cccgtctaat
     1981 tccatttttc actccccaca ctgttcaacg tcaaaacaca ttgtcattca gtcattttgg
     2041 aaaaagcacc ccgagcgcag aagcagtagg aaaaacgagt ctcgatgggg gtaggttgaa
     2101 cgagcaaaaa aaaccaattc ggctaattga actcgacttt ttctcccaat tttactctca
     2161 ccaatcgtga tgcgtatcta aatggatacg caaagaaagg aaaagaaaga gctttttatt
     2221 ttcagatcca cccacggaca cgtttttggt agaactatta catcactttt tcttagtttt
     2281 ttctgtgagc ttgatgagaa ctgtggtgta ttttttgtag ataaaacaaa atttaaacct
     2341 acttcataag ttcacacacc taccttatgt cagcctagaa agttaccacg atgatggcag
     2401 aaagttgcct ctttgcctct aaatgccgag tagtttcaaa aaatgttgac atttgatttg
     2461 gaatttaaga gtcctctctg aaaatgtgta attctgaaat tttagtgccc atttcaaaaa
     2521 aataacatct gctcagcaaa ttgtatttca tcctctcttt attttttgtt accccgtctt
     2581 acttcctctc ttttttattt attgtttttt cttgtggtcc taataataaa aatgaaaaga
     2641 gaagctgttg tgctcttcca caacaatgtg ctcttttcaa tattctaaaa tggcaatgga
     2701 attggcaatt gttggagggt taaaattgcc agacaaaagt catcttctct atgtttgttc
     2761 ttcatcttct tttacctcta ttccttatcc ttctcaataa catcctgaaa ctgacgattt
     2821 aatgagtata cttccgttta tgaatgtatc atgataattt catgtctgaa aaagaacgga
     2881 gaaatgagaa tgcatattct caacttgaac gtatagccaa gacttatgcc tattttcaat
     2941 ctgtttagac cctgacaaac atttgaccac acaggagtgt ttgggacaac aaccatattt
     3001 ttgaaaacat ttttgaatga gaaatgctat cttctgtggg aagttttaaa gcaagctgat
     3061 ttctttatat agcccaaaat aattattttt taatagatta tgcaaattaa aatatggagc
     3121 ttgatttgct gaagtgtcta tttgatttga tttgatttga tttgatttat tacgattgga
     3181 atcgtgatgg aataaaagca gtatagtata aagacagaaa aaataaatac acaatacata
     3241 ggaagaataa tcaaaaagtt acgaataaag tacaaacaag aaaagcaatt atcacctgca
     3301 aaagttgatt aattggaaca ttaaagtgaa catgaaatag gctatttggg ggtgtgagga
     3361 gagtgggtga atgatgggta ataagaggtg ccaatataag attcgtcatg ctaatttttc
     3421 atagaacttt agaccctatt tgtgaaaaag tgtcttctta gtttggtttt gcatttcccc
     3481 cagatgaact tggttttggt tctggttttg ttgtttttat cgattttgaa aaattggtcc
     3541 gtgtcaatgt caactttatt taactttaag ctttaaagaa taaacttatc aaaataaatc
     3601 aaacggtact ttttttttga aatttttatt caaaattcaa tcttttcaag agcaaactgt
     3661 aatttttttt gtcattccta tcgcaaataa acgtaaaatt cagttaattt aaaatgtgag
     3721 aaatgaggta acaactacta tagaaaaata atgaaatgtt caagcgatga gcaattaaaa
     3781 tttacgctca aaaattaacc atgatgagaa aaaaattctg ctcttcaagt tatacaagac
     3841 ttatgagtgt taactttata cttcaaaaaa taaattgtat tgaaacacct tttttagaga
     3901 ttttagatta ttagattgag aactgtacaa aaaaacgagt gttccaagat acatgagaat
     3961 gatattttaa cccgaaacaa tgaaacattc aatcatacat attctcatcc ttttcaactc
     4021 gtcaacattc cacgccttgt ctcacttttt tctatttccc gtccactggg attttgattt
     4081 cccgcccgtt tcgtctcggc attttttttt tgaaagaggt tactctctca tctttgacac
     4141 catcttccat aaaacacgag aaatgaataa tggaatcgag ggaggttctt gtttgttttt
     4201 tttgtgtgtt tttgttttgc attttactca tattttagaa cttcgaaatc tgatcatgac
     4261 tcgttttgcg agaaccattg ctttaaaata tttttatttt cttgaggata actgtttttt
     4321 tgaaaaaaaa aacatggaat ctttggaagt atgctgtaaa ttgaggaagt cttaaaatgt
     4381 ttcaacttcc atatgaaaaa ttaaatataa tacagttttg tttccgttac aaaatccaag
     4441 acgtcttctc acaaaaaatc gcctaaattt tcatttaaaa atgtgtcttt gcaaaagtta
     4501 ccagtcctgt caaatactcg caacaaatat ccaagaataa ctgactgatc tgatttttgc
     4561 agttagtttt tcctcttcaa actcaaaatc tgtttttatt tgaaccattc aatagtttaa
     4621 accccatttg aatacgtttt ctcatctttc ctcgtcacca gtttcaatgc tttaaaaaca
     4681 attttcagca tcccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca
     4741 taacatagaa cattttcagg aggacccttg gctagcgtcg acggatcccc atgctcggct
     4801 cctccagcaa tcctgaaccg ccactcctgt cgcggataat cggagtacct ccaccgccgc
     4861 cgccacgagc accgaccaca gctctcgtcc ttcgggtgcc caccgtcgac agtccatcga
     4921 aaaagaaaca acagccagac actaggaaca aatatcagga aactgcgctc agggataaaa
     4981 aaactcggac accacttgaa aaagccagac atctcgacaa tcttccctcg tatcaggccc
     5041 aaaaactatt agacggttcg caccagcttc gcatcgactc ccaccacgtt ggtagtgcag
     5101 gtcacggtgc aggacaaggt cacggacaca agaaagagtt tggacctgca atgattctct
     5161 attatttagc ttctgctttt cgggcgctct atccgcgtct agatgatgat tttgtggaca
     5221 aacttaatta ttattatact actacaatat tagcttcctt tgcacttctt gtttccgcca
     5281 agcagtatgt cggttttccc atccaatgtt gggtgcccgc cacattcaca gatgccatgg
     5341 agcagtacac tgagaactac tgctgggtac agaacacata ctgggttccc atgcaagagg
     5401 acattccacg tgaaatttat tcgcgtagaa atcgacaaat tggctattac caatgggtgc
     5461 cgtttattct tgctattgag gccttgttgt tctacgttcc atgcattctc tggagaggat
     5521 tgttgtattg gcacagtggt atcaacctac aagggctagt ccagatggcg tgtgacgcac
     5581 gactgatgga ttcggaaata aagacgagaa cagtctacac aatggcccgg cacatgcaag
     5641 acgaggttca gctcaccaat atcgaccggc aaggacacag tcggagttgt ttctcgaatt
     5701 tgcaacttgg cgcaaactgt gggagacatt gtggttgcta cgtcactatg ctctacatag
     5761 gaatcaaggt tctctactca gcaaatgtgt tgctccagtt tttcttgctg aaccatcttc
     5821 ttgggtctaa cgacttggct tatggattct ctttgttgaa agacttgatg cacgcgattg
     5881 agtgggaaca gactggaatg ttccccaggg tcacactttg tgattttgag gttcgagttc
     5941 tgggaaacat tcatcgacat accgttcagt gcgtgttaat gatcaatatg tttaatgaga
     6001 aaattttctt attcctctgg ttttggttct taacctgtgg aattgttacc gtatgtaaca
     6061 ctatgtactg gattctcatt atgttcattc cgtctcaggg aatgtcattt gtccgcaaat
     6121 acctccgtgt tctacctgac caccctgcga aaccaatagc tgatgacgtc acgctgagga
     6181 agttcactaa taacttcctc cgcaaagatg gagtcttcat gttgcgaatg atttccacac
     6241 acgccggcga actgatgagc agtgagctca ttcttgctct ctggcaggat ttcaacaacg
     6301 tcgaccgcag tccgacgcaa ttttgggatg cggaacacgg tcaagggacg atagactgac
     6361 ggatccccga gatcttcagc gctcagcatt cgtagaattg taagtttaaa ctttctcata
     6421 ctaactaacg cgctctattt aaattttcag aattcgctgt ctcatcctac tttcacctag
     6481 ttaactgctt gtcttaaaat ctatgcttct ctttagtatc taaaattttc ctagaagctt
     6541 acaagtatat aaatggtctc ttctcaataa aggttgtata tttattcatc ttattgaatc
     6601 tgccatttcc tcgtttttgc gagtttatat accttccaat tttctttcta ttgtattttc
     6661 aacttctaat tttaattcag ggaaactgct tcaacgcatc ggtaccctat ggtctcaaag
     6721 ggtgaagaag ataacatggc aattattaaa gagtttatgc gtttcaaggt gcatatggag
     6781 ggatctgtca atgggcatga gtttgaaatt gaaggtgaag gagaaggccg accatatgag
     6841 ggaacacaaa ccgcaaaact aaaggtaagt ttaaacatat atatactaac taaccctgat
     6901 tatttaaatt ttcaggtaac taaaggcgga ccattaccat tcgcctggga catcctctct
     6961 ccacagttca tgtatggaag taaagcttat gttaaacatc cggcagatat accagattat
     7021 ttgaaacttt cattcccgga gggttttaag tgggaacgcg taatgaattt tgaagacgga
     7081 ggagttgtta cagtgacgca agactcaagg taagtttaaa cagttcggta ctaactaacc
     7141 atacatattt aaattttcag cctccaagat ggagaattta tttataaagt caaacttcga
     7201 ggaacgaatt tcccctcgga tggatctgtt atgcagaaga agactatggg atgggaagct
     7261 tcaagtgaaa gaatgtaccc tgaagacggt gctcttaagg gagagattaa acaacgtctt
     7321 aaattgaaag atggaggaca ttacgatgct gaggtaagtt taaacatgat tttactaact
     7381 aactaatctg atttaaattt tcaggtgaag acaacttaca aagccaaaaa accagttcag
     7441 ctgccaggag cgtacaatgt taatattaaa ctggatatca cctcccacaa cgaggattac
     7501 actatcgttg agcaatatga aagagctgaa gggcggcact cgacaggtgg catggatgaa
     7561 ttgtataagt aggagctccc gagatcttca gcgctcagca ttcgtagaat tgtaagttta
     7621 aactttctca tactaactaa cgcgctctat ttaaattttc agaattccaa ctgagcgccg
     7681 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     7741 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     7801 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     7861 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     7921 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     7981 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     8041 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     8101 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     8161 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     8221 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     8281 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     8341 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     8401 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     8461 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     8521 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     8581 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     8641 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     8701 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     8761 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     8821 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     8881 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8941 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9001 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9061 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9121 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9181 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9241 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9301 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9361 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9421 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9481 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9541 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9601 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9661 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9721 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9781 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9841 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9901 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9961 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10021 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10081 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10141 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10201 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10261 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10321 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10381 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10441 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10501 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10561 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10621 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10681 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10741 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagag
//
