LOCUS       sequence-354946-       11092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in 
            human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Joshi J, Xu B, Rubart M, Chang Y, Bao X, Chaliki HP, Scott LR, Zhu W
  TITLE     Optogenetic Control of Engrafted Human Induced Pluripotent Stem 
            Cell-Derived Cardiomyocytes in Live Mice: A Proof-of-Concept Study.
  JOURNAL   Cells. 2022 Mar 10;11(6). pii: cells11060951. doi: 
            10.3390/cells11060951.
  PUBMED    35326403
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2022-03-10"; volume: "11(6). pii: cells11060951. doi"; pages:
            " 10.3390/cells11060951"
COMMENT     Sequence Label: AAVS1-Puro cTnT CCND2-T2A-iChloC
FEATURES             Location/Qualifiers
     source          1..11092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             955..1554
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(955..974)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1451..1471
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1586..1603)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1592..1816
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2460..2478
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2510..2534
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2547..2556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3429..3482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3483..4409
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEQIYVCAIRMVKVILEFFFSFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSNIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     misc_feature    4449..5037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4502..4522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4920..4931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5069..5545
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5268..5287)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5655..5674)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5720..5775
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5774..5793)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6136..6152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6160..6176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6184..6190,6191..6208,6209..6214))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6229..6250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6324..7160
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(7195..7213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7220..7237)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7220..7236)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7229..7251)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7374..7676
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7555..7574
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(8079..8098)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8693..8712
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9073..9864,9865..9933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9696..9715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10057..10645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10546..10565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10799..10816
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10933..10954
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10969..10974,10975..10992,10993..10999)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11007..11023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11031..11047
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11068..11086
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc
      961 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     1021 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     1081 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     1141 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     1201 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     1261 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     1321 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     1381 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     1441 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     1501 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga
     1561 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1621 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1681 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1741 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1801 gcggtgggct ctatgggtct cgacattgat tattgactag tgtcatggag aagacccacc
     1861 ttgcagatgt cctcactggg gctggcagag ccggcaacct gcccaaggct gctcagtcca
     1921 ttaggagcca gtagcctgga agatgtcttt acccccagca tcagttcaag tggagcagca
     1981 cataactctt gccctctgcc ttccaagatt ctggtgctga gacttatgga gtgtcttgga
     2041 ggttgccttc tgccccccaa ccctgctccc agctggccct cccaggcctg ggttgctggc
     2101 ctctgcttta tcaggattct caagagggac agctggttta tgttgcatga ctgttccctg
     2161 catatctgct ctggttttaa atagcttatc tgagcagctg gaggaccaca tgggcttata
     2221 tggcgtgggg tacatgttcc tgtagccttg tccctggcac ctgccaaaat agcagccaac
     2281 accccccacc cccaccgcca tccccctgcc ccacccgtcc cctgtcgcac attcctccct
     2341 ccgcagggct ggctcaccag gccccagccc acatgcctgc ttaaagccct ctccatcctc
     2401 tgcctcaccc agtccccgct gagactgagc agacgcctcc aggatccgcc accctcgagt
     2461 aatacgactc actataggga gacccaagct ggctaggtaa gcttgatcaa caagtttgta
     2521 caaaaaagca ggctgtcgac ggtaccgcca ccatggagct gctgtgccac gaggtggacc
     2581 cggtccgcag ggccgtgcgg gaccgcaacc tgctccgaga cgaccgcgtc ctgcagaacc
     2641 tgctcaccat cgaggagcgc taccttccgc agtgctccta cttcaagtgc gtgcagaagg
     2701 acatccaacc ctacatgcgc agaatggtgg ccacctggat gctggaggtc tgtgaggaac
     2761 agaagtgcga agaagaggtc ttccctctgg ccatgaatta cctggaccgt ttcttggctg
     2821 gggtcccgac tccgaagtcc catctgcaac tcctgggtgc tgtctgcatg ttcctggcct
     2881 ccaaactcaa agagaccagc ccgctgaccg cggagaagct gtgcatttac accgacaact
     2941 ccatcaagcc tcaggagctg ctggagtggg aactggtggt gctggggaag ttgaagtgga
     3001 acctggcagc tgtcactcct catgacttca ttgagcacat cttgcgcaag ctgccccagc
     3061 agcgggagaa gctgtctctg atccgcaagc atgctcagac cttcattgct ctgtgtgcca
     3121 cggactttaa gtttgccatg tacccaccgt cgatgatcgc aactggaagt gtgggagcag
     3181 ccatctgtgg gctccagcag gatgaggaag tgagctcgct cacttgtgat gccctgactg
     3241 agctgctggc taagatcacc aacacagacg tggattgtct caaagcttgc caggagcaga
     3301 ttgaggcggt gctcctcaat agcctgcagc agtaccgtca ggaccaacgt gacggatcca
     3361 agtcggagga tgaactggac caagccagca cccctacaga cgtgcgggat atcgacctgg
     3421 gcagcggaga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     3481 ctatggatta tggaggcgcc ctgagtgccg ttgggcgcga gctgctattt gtaacgaacc
     3541 cagtagtcgt caatggctct gtacttgtgc ctgaggacca gtgttactgc gcgggctgga
     3601 ttgagtcgcg tggcacaaac ggtgcccaaa cggcgtcgaa cgtgctgcaa tggcttgctg
     3661 ctggcttctc catcctactg cttatgtttt acgcctacca aacatggaag tcaacctgcg
     3721 gctgggagca gatctatgtg tgcgctatca ggatggtcaa ggtgattctc gagttcttct
     3781 tctcgtttaa gaacccgtcc atgctgtatc tagccacagg ccaccgcgtc cagtggttgc
     3841 gttacgccga gtggcttctc acctgcccgg tcattctcat tcacctgtca aacctgacgg
     3901 gcttgtccaa cgactacagc aggcgcacca tgggtctgct tgtgtctaat attggctgta
     3961 ttgtgtgggg cgccacttcc gccatggcca ccggatacgt caaggtcatc ttcttctgcc
     4021 tgggtctgtg ttatggtgct aacacgttct ttcacgctgc caaggcctac atcgagggtt
     4081 accacaccgt gccgaagggc cggtgtcgcc aggtggtgac tggcatggct tggctcttct
     4141 tcgtatcatg gggtatgttc cccatcctgt tcatcctcgg ccccgagggc ttcggcgtcc
     4201 tgagcgtgta cggctccacc gtcggccaca ccatcattga cctgatgtcg aagaactgct
     4261 ggggtctgct cggccactac ctgcgcgtgc tgatccacga gcatatcctc atccacggcg
     4321 acattcgcaa gaccaccaaa ttgaacattg gtggcactga gattgaggtc gagacgctgg
     4381 tggaggacga ggccgaggct ggcgcggtat aaactagtac gcgtgaattc gatatcaagc
     4441 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4501 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4561 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4621 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4681 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4741 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4801 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4861 ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4921 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4981 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     5041 gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc
     5101 tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag
     5161 ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt
     5221 atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc
     5281 aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc
     5341 gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag
     5401 ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa
     5461 ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg
     5521 aaccactgct cccttccctg tccttctgat tttgtaggta accaggcgcg tgaattcact
     5581 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
     5641 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     5701 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     5761 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     5821 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     5881 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     5941 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     6001 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     6061 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     6121 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6181 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6241 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6301 gcggatcgac agtactaagc tttactaggg acaggattgg tgacagaaaa gccccatcct
     6361 taggcctcct ccttcctagt ctcctgatat tgggtctaac ccccacctcc tgttaggcag
     6421 attccttatc tggtgacaca cccccatttc ctggagccat ctctctcctt gccagaacct
     6481 ctaaggtttg cttacgatgg agccagagag gatcctggga gggagagctt ggcagggggt
     6541 gggagggaag ggggggatgc gtgacctgcc cggttctcag tggccaccct gcgctaccct
     6601 ctcccagaac ctgagctgct ctgacgcggc tgtctggtgc gtttcactga tcctggtgct
     6661 gcagcttcct tacacttccc aagaggagaa gcagtttgga aaaacaaaat cagaataagt
     6721 tggtcctgag ttctaacttt ggctcttcac ctttctagtc cccaatttat attgttcctc
     6781 cgtgcgtcag ttttacctgt gagataaggc cagtagccag ccccgtcctg gcagggctgt
     6841 ggtgaggagg ggggtgtccg tgtggaaaac tccctttgtg agaatggtgc gtcctaggtg
     6901 ttcaccaggt cgtggccgcc tctactccct ttctctttct ccatccttct ttccttaaag
     6961 agtccccagt gctatctggg acatattcct ccgcccagag cagggtcccg cttccctaag
     7021 gccctgctct gggcttctgg gtttgagtcc ttggcaagcc caggagaggc gctcaggctt
     7081 ccctgtcccc cttcctcgtc caccatctca tgcccctggc tctcctgccc cttccctaca
     7141 ggggttcctg gctctgctct aagggcgaat tcgcggccgc taaattcaat tcgccctata
     7201 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7261 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7321 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctatacgta cggcagttta
     7381 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     7441 ttattgacac gccggggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     7501 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     7561 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     7621 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     7681 caggcatgag attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc
     7741 cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa
     7801 acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact
     7861 gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg
     7921 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     7981 ggggatcaag ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     8041 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     8101 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     8161 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     8221 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     8281 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     8341 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     8401 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     8461 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     8521 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     8581 tgacccatgc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     8641 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     8701 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     8761 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     8821 tgaattatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
     8881 atttcacacc gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8941 atttttctaa atacattcaa atatgtatcc gctcatgaga ttatcaaaaa ggatcttcac
     9001 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9061 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9121 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9181 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9241 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9301 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9361 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9421 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9481 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9541 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9601 aagatgcttt tctgtgactg gtgaatactc aaccaagtca ttctgagaat agtgtatgcg
     9661 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9721 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9781 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9841 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9901 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9961 catttatcag ggttattgtc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10021 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10081 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10141 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10201 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10261 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10321 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10381 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10441 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10501 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10561 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10621 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10681 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    10741 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    10801 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    10861 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    10921 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    10981 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    11041 ctatgaccat gattacgcca agctcagaat taaccctcac taaagggact ag
//
