LOCUS       sequence-354964-       13373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human EGFR C-terminally labeled with FusionRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13373)
  AUTHORS   Farahani PE, Lemke SB, Dine E, Uribe G, Toettcher JE, Nelson CM
  TITLE     Substratum stiffness regulates Erk signaling dynamics through 
            receptor-level control.
  JOURNAL   Cell Rep. 2021 Dec 28;37(13):110181. doi: 
            10.1016/j.celrep.2021.110181.
  PUBMED    34965432
REFERENCE   2  (bases 1 to 13373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-12-28"; volume: "37(13):110181. doi"; pages: " 
            10.1016/j.celrep.2021.110181"
COMMENT     Sequence Label: EGFR-FR
FEATURES             Location/Qualifiers
     source          1..13373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             253..297
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    582..699
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        852..1259
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(1325..1396,1397..3259,3260..3328,3329..4954)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             4964..5659
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    5769..6357
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5822..5842)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6240..6251)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6360..6376)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6361..6377)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6467..6700
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6725..6743)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6726..6743)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7033..7052)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7266..7285)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7385..7407
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7445..7463)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7531..7635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7636..7704,7705..8496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8667..9255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9156..9175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9409..9426
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9496..9516)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9501..9830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9681..9816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10964..11029
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11159..11179
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11604..11738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11641..11660)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11695..11714
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11906..12539
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12586..12711
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(13208..13373,1..68)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
       61 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
      121 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
      181 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
      241 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
      301 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
      361 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
      421 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
      481 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
      541 ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg
      601 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
      661 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
      721 agagatccag tttggatcga taagcttgat atcgaattcc tgcagccccg ataaaataaa
      781 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
      841 agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca agaatagaga
      901 agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca ggatatctgc
      961 ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca gtttcggccc
     1021 cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc agtttcttaa
     1081 gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg ccttatttga
     1141 attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc gagctctata
     1201 aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag tcgcccgggg
     1261 gggatctgga gctctcgaga attctcacgc gtcaagtgga gcaaggcagg tggacagtgg
     1321 atccatgcga ccctccggga cggccggggc agcgctcctg gcgctgctgg ctgcgctctg
     1381 cccggcgagt cgggctctgg aggaaaagaa agtttgccaa ggcacgagta acaagctcac
     1441 gcagttgggc acttttgaag atcattttct cagcctccag aggatgttca ataactgtga
     1501 ggtggtcctt gggaatttgg aaattaccta tgtgcagagg aattatgatc tttccttctt
     1561 aaagaccatc caggaggtgg ctggttatgt cctcattgcc ctcaacacag tggagcgaat
     1621 tcctttggaa aacctgcaga tcatcagagg aaatatgtac tacgaaaatt cctatgcctt
     1681 agcagtctta tctaactatg atgcaaataa aaccggactg aaggagctgc ccatgagaaa
     1741 tttacaggaa atcctgcatg gcgccgtgcg gttcagcaac aaccctgccc tgtgcaacgt
     1801 ggagagcatc cagtggcggg acatagtcag cagtgacttt ctcagcaaca tgtcgatgga
     1861 cttccagaac cacctgggca gctgccaaaa gtgtgatcca agctgtccca atgggagctg
     1921 ctggggtgca ggagaggaga actgccagaa actgaccaaa atcatctgtg cccagcagtg
     1981 ctccgggcgc tgccgtggca agtcccccag tgactgctgc cacaaccagt gtgctgcagg
     2041 ctgcacaggc ccccgggaga gcgactgcct ggtctgccgc aaattccgag acgaagccac
     2101 gtgcaaggac acctgccccc cactcatgct ctacaacccc accacgtacc agatggatgt
     2161 gaaccccgag ggcaaataca gctttggtgc cacctgcgtg aagaagtgtc cccgtaatta
     2221 tgtggtgaca gatcacggct cgtgcgtccg agcctgtggg gccgacagct atgagatgga
     2281 ggaagacggc gtccgcaagt gtaagaagtg cgaagggcct tgccgcaaag tgtgtaacgg
     2341 aataggtatt ggtgaattta aagactcact ctccataaat gctacgaata ttaaacactt
     2401 caaaaactgc acctccatca gtggcgatct ccacatcctg ccggtggcat ttaggggtga
     2461 ctccttcaca catactcctc ctctggatcc acaggaactg gatattctga aaaccgtaaa
     2521 ggaaatcaca gggtttttgc tgattcaggc ttggcctgaa aacaggacgg acctccatgc
     2581 ctttgagaac ctagaaatca tacgcggcag gaccaagcaa catggtcagt tttctcttgc
     2641 agtcgtcagc ctgaacataa catccttggg attacgctcc ctcaaggaga taagtgatgg
     2701 agatgtgata atttcaggaa acaaaaattt gtgctatgca aatacaataa actggaaaaa
     2761 actgtttggg acctccggtc agaaaaccaa aattataagc aacagaggtg aaaacagctg
     2821 caaggccaca ggccaggtct gccatgcctt gtgctccccc gagggctgct ggggcccgga
     2881 gcccagggac tgcgtctctt gccggaatgt cagccgaggc agggaatgcg tggacaagtg
     2941 caaccttctg gagggtgagc caagggagtt tgtggagaac tctgagtgca tacagtgcca
     3001 cccagagtgc ctgcctcagg ccatgaacat cacctgcaca ggacggggac cagacaactg
     3061 tatccagtgt gcccactaca ttgacggccc ccactgcgtc aagacctgcc cggcaggagt
     3121 catgggagaa aacaacaccc tggtctggaa gtacgcagac gccggccatg tgtgccacct
     3181 gtgccatcca aactgcacct acggatgcac tgggccaggt cttgaaggct gtccaacgaa
     3241 tgggcctaag atcccgtcca tcgccactgg gatggtgggg gccctcctct tgctgctggt
     3301 ggtggccctg gggatcggcc tcttcatgcg aaggcgccac atcgttcgga agcgcacgct
     3361 gcggaggctg ctgcaggaga gggagcttgt ggagcctctt acacccagtg gagaagctcc
     3421 caaccaagct ctcttgagga tcttgaagga aactgaattc aaaaagatca aagtgctggg
     3481 ctccggtgcg ttcggcacgg tgtataaggg actctggatc ccagaaggtg agaaagttaa
     3541 aattcccgtc gctatcaagg aattaagaga agcaacatct ccgaaagcca acaaggaaat
     3601 cctcgatgaa gcctacgtga tggccagcgt ggacaacccc cacgtgtgcc gcctgctggg
     3661 catctgcctc acctccaccg tgcaactcat cacgcagctc atgcccttcg gctgcctcct
     3721 ggactatgtc cgggaacaca aagacaatat tggctcccag tacctgctca actggtgtgt
     3781 gcagatcgca aagggcatga actacttgga ggaccgtcgc ttggtgcacc gcgacctggc
     3841 agccaggaac gtactggtga aaacaccgca gcatgtcaag atcacagatt ttgggctggc
     3901 caaactgctg ggtgcggaag agaaagaata ccatgcagaa ggaggcaaag tgcctatcaa
     3961 gtggatggca ttggaatcaa ttttacacag aatctatacc caccagagtg atgtctggag
     4021 ctacggggtg accgtttggg agttgatgac ctttggatcc aagccatatg acggaatccc
     4081 tgccagcgag atctcctcca tcctggagaa aggagaacgc ctccctcagc cacccatatg
     4141 taccatcgat gtctacatga tcatggtcaa gtgctggatg atagacgcag atagtcgccc
     4201 aaagttccgt gagttgatca tcgaattctc caaaatggcc cgagaccccc agcgctacct
     4261 tgtcattcag ggggatgaaa gaatgcattt gccaagtcct acagactcca acttctaccg
     4321 tgccctgatg gatgaagaag acatggacga cgtggtggat gccgacgagt acctcatccc
     4381 acagcagggc ttcttcagca gcccctccac gtcacggact cccctcctga gctctctgag
     4441 tgcaaccagc aacaattcca ccgtggcttg cattgataga aatgggctgc aaagctgtcc
     4501 catcaaggaa gacagcttct tgcagcgata cagctcagac cccacaggcg ccttgactga
     4561 ggacagcata gacgacacct tcctcccagt gcctgaatac ataaaccagt ccgttcccaa
     4621 aaggcccgct ggctctgtgc agaatcctgt ctatcacaat cagcctctga accccgcgcc
     4681 cagcagagac ccacactacc aggaccccca cagcactgca gtgggcaacc ccgagtatct
     4741 caacactgtc cagcccacct gtgtcaacag cacattcgac agccctgccc actgggccca
     4801 gaaaggcagc caccaaatta gcctggacaa ccctgactac cagcaggact tctttcccaa
     4861 ggaagccaag ccaaatggca tctttaaggg ctccacagct gaaaatgcag aatacctaag
     4921 ggtcgcgcca caaagcagtg aatttattgg agcaggtgga ggaatggtga gcgagctgat
     4981 taaggagaac atgcccatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
     5041 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     5101 cgtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
     5161 cagcagaacc ttcatcaagc accctccggg catccccgac ttctttaagc agtccttccc
     5221 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     5281 ccaggacacc agcctccagg acggctgcct catctacaac gtcaaggtta gaggggtgaa
     5341 cttcccagcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg cctccaccga
     5401 gacgatgtac cccgctgacg gcggcctgga aggcgcatgt gacatggccc tgaagctcgt
     5461 gggcgggggc cacctgatct gcaaccttga gaccacatac agatccaaga aacccgctac
     5521 gaacctcaag atgcccggcg tctacaacgt ggaccacaga ctggaaagaa tcaaggaggc
     5581 cgacgatgag acctacgtcg agcagcacga ggtggctgtg gccagatact ctactggtgg
     5641 cgctggtgat ggaggtaaag cggccacgcg tgcggccgca actcccacct gcaacatgcg
     5701 tgactgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
     5761 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5821 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5881 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5941 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6001 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6061 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6121 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6181 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6241 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6301 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     6361 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
     6421 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6481 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     6541 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6601 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6661 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     6721 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     6781 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     6841 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     6901 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     6961 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     7021 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     7081 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     7141 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     7201 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     7261 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     7321 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7381 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     7441 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7501 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7561 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7621 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7681 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7741 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7801 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7861 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7921 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7981 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8041 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8101 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8161 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8221 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8281 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8341 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8401 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8461 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8521 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8581 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8641 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8701 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8761 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8821 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8881 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8941 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9001 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9061 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9301 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9361 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9421 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9481 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     9541 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9601 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     9661 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9721 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     9781 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     9841 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     9901 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     9961 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
    10021 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
    10081 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
    10141 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
    10201 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
    10261 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
    10321 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
    10381 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
    10441 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
    10501 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
    10561 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
    10621 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
    10681 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
    10741 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
    10801 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
    10861 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
    10921 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
    10981 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
    11041 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
    11101 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
    11161 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
    11221 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
    11281 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
    11341 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
    11401 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
    11461 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
    11521 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    11581 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    11641 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11701 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    11761 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    11821 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    11881 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    11941 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    12001 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    12061 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    12121 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    12181 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    12241 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    12301 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    12361 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    12421 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    12481 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    12541 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    12601 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    12661 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    12721 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
    12781 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
    12841 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
    12901 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
    12961 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
    13021 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
    13081 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
    13141 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
    13201 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact ataggcgcag
    13261 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
    13321 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtc
//
