LOCUS       sequence-354966-       13092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one prime editor piggyBac transposon, NG variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Eggenschwiler R, Gschwendtberger T, Felski C, Jahn C, Langer F, 
            Sterneckert J, Hermann A, Luhmann J, Steinemann D, Haase A, Martin 
            U, Petri S, Cantz T
  TITLE     A selectable all-in-one CRISPR prime editing piggyBac transposon 
            allows for highly efficient gene editing in human cell lines.
  JOURNAL   Sci Rep. 2021 Nov 12;11(1):22154. doi: 10.1038/s41598-021-01689-2.
  PUBMED    34773059
REFERENCE   2  (bases 1 to 13092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-11-12"; volume: "11(1):22154. doi"; pages: " 
            10.1038/s41598-021-01689-2"
COMMENT     Sequence Label: PB-PE NG
FEATURES             Location/Qualifiers
     source          1..13092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          117..1133
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1057..1079
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1141..1160
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1199..1218
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1199..1217
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1267..1287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1288..5388
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7561..7581
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7591..7647
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7648..8244
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7648..7667)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8144..8164
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             8251..9246
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(9268..9285)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9274..9498
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   9682..9744
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(9810..10398)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9890..9909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10569..11360,11361..11429))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11192..11211
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11430..11534)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11602..11620
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11658..11680)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11780..11799
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     repeat_region   11823..11857
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        12202..12450
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     12202..12222
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     12373..12392
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        12479..12554
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        12600..12979
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
       61 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      121 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      181 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      241 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
      301 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
      361 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
      421 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
      481 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
      541 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
      601 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
      661 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
      721 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
      781 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
      841 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
      901 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
      961 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     1021 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1081 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1141 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgata gcggccgcta
     1201 atacgactca ctatagggag agccgccacc atgaaacgga cagccgacgg aagcgagttc
     1261 gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca gcatcggcct ggacatcggc
     1321 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
     1381 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
     1441 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
     1501 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
     1561 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
     1621 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
     1681 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
     1741 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
     1801 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
     1861 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg
     1921 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag
     1981 aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc
     2041 aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac
     2101 gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc
     2161 gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc
     2221 accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg
     2281 accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc
     2341 gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc
     2401 tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag
     2461 ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac
     2521 cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc
     2581 ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg
     2641 ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc
     2701 atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc
     2761 gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc
     2821 ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag
     2881 ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg
     2941 ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc
     3001 gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc
     3061 acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac
     3121 gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc
     3181 gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag
     3241 cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac
     3301 aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac
     3361 ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag
     3421 gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc
     3481 attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc
     3541 cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag
     3601 ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc
     3661 agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac
     3721 ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg
     3781 ctgtccgact acgatgtgga cgctatcgtg cctcagagct ttctgaagga cgactccatc
     3841 gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc
     3901 gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt
     3961 acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat
     4021 aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca
     4081 cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa
     4141 gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt
     4201 tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc
     4261 gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac
     4321 tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct
     4381 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg
     4441 gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc
     4501 gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg
     4561 aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatccggccc
     4621 aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc
     4681 ggcttcgtta gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc
     4741 aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc
     4801 agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag
     4861 gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
     4921 atgctggcct ctgcccggtt cctgcagaag ggaaacgaac tggccctgcc ctccaaatat
     4981 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
     5041 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
     5101 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
     5161 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
     5221 tttaccctga ccaatctggg agcccctcgg gccttcaagt actttgacac caccatcgac
     5281 cggaaggtgt accggagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
     5341 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgactc tggaggatct
     5401 agcggaggat cctctggcag cgagacacca ggaacaagcg agtcagcaac accagagagc
     5461 agtggcggca gcagcggcgg cagcagcacc ctaaatatag aagatgagta tcggctacat
     5521 gaaacctcaa aagagccaga tgtttctcta gggtccacat ggctgtctga ttttcctcag
     5581 gcctgggcgg aaaccggggg catgggactg gcagttcgcc aagctcctct gatcatacct
     5641 ctgaaagcaa cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga
     5701 ctggggatca agccccacat acagagactg ttggaccagg gaatactggt accctgccag
     5761 tccccctgga acacgcccct gctacccgtt aagaaaccag ggactaatga ttataggcct
     5821 gtccaggatc tgagagaagt caacaagcgg gtggaggaca tccaccccac cgtgcccaac
     5881 ccttacaacc tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgattta
     5941 aaggatgcct ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag
     6001 tggagagatc cagagatggg aatctcagga caattgacct ggaccagact cccacagggt
     6061 ttcaaaaaca gtcccaccct gtttaatgag gcactgcaca gggacctagc agacttcaga
     6121 atccagcacc cagacttgat cctgctacag tacgtggatg acttactgct ggccgccact
     6181 tctgagctag actgccaaca aggtactcgg gccctgttac aaaccctagg gaacctcggg
     6241 tatcgggcct cggccaagaa agcccaaatt tgccagaaac aggtcaagta tctggggtat
     6301 cttctaaaag agggtcagag atggctgact gaggccagaa aagagactgt gatggggcag
     6361 cctactccca agacccctcg acaactaagg gagttcctag ggaaggcagg cttctgtcgc
     6421 ctcttcatcc ctgggtttgc agaaatggca gcccccctgt accctctcac caaaccgggg
     6481 actctgttta attggggccc agaccaacaa aaggcctatc aagaaatcaa gcaagctctt
     6541 ctaactgccc cagccctggg gttgccagat ttgactaagc cctttgaact ctttgtcgac
     6601 gagaagcagg gctacgccaa aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg
     6661 gtggcctacc tgtccaaaaa gctagaccca gtagcagctg ggtggccccc ttgcctacgg
     6721 atggtagcag ccattgccgt actgacaaag gatgcaggca agctaaccat gggacagcca
     6781 ctagtcattc tggcccccca tgcagtagag gcactagtca aacaaccccc cgaccgctgg
     6841 ctttccaacg cccggatgac tcactatcag gccttgcttt tggacacgga ccgggtccag
     6901 ttcggcccgg tggtagccct gaacccggct acgctgctcc cactgcctga ggaagggctg
     6961 caacacaact gccttgatat cctggccgaa gcccacggaa cccgacccga cctaacggac
     7021 cagccgctcc cagacgccga ccacacctgg tacacggatg gaagcagtct cttacaagag
     7081 ggacagcgta aggcgggagc tgcggtgacc accgagacag aggtaatctg ggctaaagcc
     7141 ctgccagccg ggacatccgc tcagcgggct gaactgatag cactcaccca ggccctaaag
     7201 atggcagaag gtaagaagct aaatgtttat actgatagcc gttatgcttt tgctactgcc
     7261 catatccatg gagaaatata cagaaggcgt gggtggctca catcagaagg caaagagatc
     7321 aaaaataaag acgagatctt ggccctacta aaagccctct ttctgcccaa aagacttagc
     7381 ataatccatt gtccaggaca tcaaaaggga cacagcgccg aggctagagg caaccggatg
     7441 gctgaccaag cggcccgaaa ggcagccatc acagagactc cagacacctc taccctcctc
     7501 atagaaaatt catcaccctc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
     7561 cccaagaaga agaggaaagt cggctccggc gctaccaatt tctccctgct caagcaagcc
     7621 ggagacgtcg aggaaaaccc aggcccaatg accgagtaca agcccacggt gcgcctcgcc
     7681 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     7741 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     7801 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     7861 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7921 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7981 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     8041 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     8101 gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct ccccttctac
     8161 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8221 tgcatgaccc gcaagcccgg tgccggctcc atgcccacgc tactgcgggt ttatatagac
     8281 ggtcctcacg ggatggggaa aaccaccacc acgcaactgc tggtggccct gggttcgcgc
     8341 gacgatatcg tctacgtacc cgagccgatg acttactggc aggtgctggg ggcttccgag
     8401 acaatcgcga acatctacac cacacaacac cgcctcgacc agggtgagat atcggccggg
     8461 gacgcggcgg tggtaatgac aagcgcccag ataacaatgg gcatgcctta tgccgtgacc
     8521 gacgccgttc tggctcctca tatcgggggg gaggctggga gctcacatgc cccgcccccg
     8581 gccctcaccc tcatcttcga ccgccatccc atcgccgccc tcctgtgcta cccggccgcg
     8641 cgatacctta tgggcagcat gaccccccag gccgtgctgg cgttcgtggc cctcatcccg
     8701 ccgaccttgc ccggcacaaa catcgtgttg ggggcccttc cggaggacag acacatcgac
     8761 cgcctggcca aacgccagcg ccccggcgag cggcttgacc tggctatgct ggccgcgatt
     8821 cgccgcgttt acgggctgct tgccaatacg gtgcggtatc tgcagggcgg cgggtcgtgg
     8881 cgggaggatt ggggacagct ttcggggacg gccgtgccgc cccagggtgc cgagccccag
     8941 agcaacgcgg gcccacgacc ccatatcggg gacacgttat ttaccctgtt tcgggccccc
     9001 gagttgctgg cccccaacgg cgacctgtac aacgtgtttg cctgggcctt ggacgtcttg
     9061 gccaaacgcc tccgtcccat gcacgtcttt atcctggatt acgaccaatc gcccgccggc
     9121 tgccgggacg ccctgctgca acttacctcc gggatggtcc agacccacgt caccaccccc
     9181 ggctccatac cgacgatctg cgacctggcg cgcacgtttg cccgggagat gggggaggct
     9241 aactgagctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     9301 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     9361 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     9421 gtggggtggg gcaggacagc aagggggagg attgggagga caatagcagg catgctgggg
     9481 atgcggtggg ctctatgggg cgcgcctaaa agttttgtta ctttatagaa gaaattttga
     9541 gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat
     9601 tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc
     9661 gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt
     9721 cacaatatga ttatctttct agggttaaac atgtgagcaa aaggccagca aaaggccagg
     9781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9841 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9901 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9961 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10021 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10081 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10141 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10201 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    10261 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10321 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10381 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10441 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10501 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10561 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10621 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10681 tctggcccca gtgctgcaat gataccgcga ctcccacgct caccggctcc agatttatca
    10741 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10801 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10861 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10921 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10981 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11041 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11101 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11161 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11221 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11281 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11341 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11401 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11461 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11521 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    11581 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    11641 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    11701 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    11761 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccaatttgtt
    11821 aaccctagaa agatagtctg cgtaaaattg acgcatgcat tcttgaaata ttgctctctc
    11881 tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc
    11941 tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga ttttgaacta taacgaccgc
    12001 gtgagtcaaa atgacgcatg attatctttt acgtgacttt taagatttaa ctcatacgat
    12061 aattatattg ttatttcatg ttctacttac gtgataactt attatatata tattttcttg
    12121 ttatagatat catgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggaccc
    12181 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
    12241 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
    12301 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
    12361 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
    12421 ttatatatct tgtggaaagg acgaaacacc gggtcttcgt ggagctcatc gaagacctgt
    12481 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
    12541 caccgagtcg gtgcagagac cgaatcgacc agaggtctcg tttttttcta gacgctagcg
    12601 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
    12661 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
    12721 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
    12781 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
    12841 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
    12901 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
    12961 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
    13021 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
    13081 gggggcgggg gg
//
