LOCUS       sequence-354969-       11621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea 
            orientalis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Wimmer F, Mougiakos I, Englert F, Beisel CL
  TITLE     Rapid cell-free characterization of multi-subunit CRISPR effectors 
            and transposons.
  JOURNAL   Mol Cell. 2022 Feb 16. pii: S1097-2765(22)00102-2. doi: 
            10.1016/j.molcel.2022.01.026.
  PUBMED    35216669
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 Feb 16. pii: S1097-2765(22)00102-2. doi: 
            10.1016/j.molcel.2022.01.026."
COMMENT     Sequence Label: pRoTnsABCDQ
FEATURES             Location/Qualifiers
     source          1..11621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(895..1977)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1939..1958
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1978..2055)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2261..2280
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2364..2382
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2383..2407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             7339..7356
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7409..7427)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7423..7470
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        7493..7511
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7512..7536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      9017..9472
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9104..9123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9314..9335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9564..10379)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9564..10379)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10287..10306
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10371..10390
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10501..11089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10990..11009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11243..11260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11275..11414
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11340..11359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11500..11522
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(11516..11621,1..86))
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
       61 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
      121 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
      181 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
      241 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
      301 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
      361 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
      421 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
      481 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
      541 atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
      601 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
      661 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
      721 gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg
      781 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
      841 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
      901 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      961 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     1021 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     1081 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     1141 gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga
     1201 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     1261 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     1321 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     1381 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     1441 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
     1501 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     1561 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     1621 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     1681 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     1741 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     1801 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     1861 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     1921 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     1981 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
     2041 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
     2101 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
     2161 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     2221 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     2281 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     2341 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
     2401 caattcccct ctaggatccc ctgtagaaat aattttgttt aactttaata aggagatata
     2461 ccatgctctc cgataccgaa tttcaaacat ggtgcgatgg tatgcaactc tcccaacgac
     2521 aaagagagtt aatcacccaa gtacgcacct ccccacccat tcgcaaagta aaaggaagtg
     2581 ccagaaacgt tcatggtcgc tatccaagta aaaaaatggg cacaacgatt caatttgaat
     2641 cccatacggt tgaattacct gcactgtgcg aattctacga atatgatgac tcggttttag
     2701 aatactggga ccaaccctat aaattcacga ttaaatgcgc tcctgaagga agacaagcca
     2761 ccacaattac tcattatcct gatttcttag tcttgagaaa aaatagctgt gggtttgaag
     2821 aatggaagaa agagacaaaa ctagaaaaac tgagtgaaaa acaaccctat cgttatcaaa
     2881 aacaagaagg aatctggatt gatactattg ttggtgaata tcttgaaaac ttaggctatt
     2941 attactgtgt tcgctcagat agagaaatag attgggttaa gtatcgtaat cagaagtatt
     3001 tacagtccta caaacacgga tatttagacc aacagtatat tattgataca gcagtagaag
     3061 aagaacttaa agatattatc aatcataatc ccgggattga cctaactact ctactaaata
     3121 aagcccaaaa cgcaacgatt gatgatatta ataccttaat tgcccttgag aaagtctatg
     3181 ttgatttaag tgtagtcccc atcgaggaac aactcgataa agtccactta tttcgtgaca
     3241 aagcaacagc agaagcctat attacggcta cccacaatta tagtgaaccg attacgggta
     3301 gtgtgcaaat cgtgaatctc aaggtggggt catctttctt actagatgga aaaagcttaa
     3361 cgattgacca tgtaggggat agtaaaatta ttcttaaagg agaacaagga attattcgtt
     3421 ggactcatgc cgaatttcaa caattactgg aactcggaga aattactcat ctggaaacag
     3481 aagaaaaacc cgctttggat gaagaaggat ggcaatattt tgtcgaggcg agtccaaaag
     3541 ccttagaaac ggctaatcaa cgatattcag cgattaaacc gcttttagat ggagaaactt
     3601 atagttcctt agacatagat atatcagcac gaactttacg ccactggaaa gctaaatatc
     3661 aaaaagcgca acaagactat ggatgtggat ttctgggttt aattcctcaa agtaaaggga
     3721 atccaacgcc tcgttattct gatgaagacg tagaatttgt cgataagata attgaagaac
     3781 aatacgaaac gtttaagcaa aagaatgctt ggcaaaccta tcttaggcta agggatagat
     3841 ggaaagaatc aggaagaact tctccgattc ctagtcatac cttttattat gaaagaatca
     3901 aaaagcgcaa tagttatcaa caaactaaaa gaagaatggg atctcgtgcg gccaataatt
     3961 tgttaagacc ttggttgatt gaattaacaa ctcctcgaca tggttctcgg ccttttgaaa
     4021 tctgccatat tgaccacaca cctctagatg ttgaatgttt atgtccttat agtaacaaga
     4081 atttaggcag accttgggta acagggatga ttgatgccta tactagaaga attttagcct
     4141 tatatctcac ctttgatgag ccaagttatc gctcttgtat gatggtgatg cggatttgtg
     4201 tgcaacggtt tggacgattt cctgaattaa ttgtagtaga taacggaaaa gaatttagtt
     4261 ctacttattt tgaaacatta ctcgctcgtt ttgaagcaac caaaaaacat cggcctaaag
     4321 atgtgccgaa atttagttca attattgagc gttggtttgg gtcacagaat cgagaattta
     4381 ttaataactt gagaggaaat acccaaatta tgaagcacgt tcgcctcgtc aaaaaggaga
     4441 ataatccgaa aaatttggca acatggacat tagatgaact ctatgattat ttagcctttg
     4501 gttatgctta tggggtttat gataggatgc aacaccctgc tttagaagga atgtctcctc
     4561 aacaagcgta tgaattagga ttagcaaaaa caggacatcg accccatcaa tatattaagt
     4621 atgatgagca atttaagatt ttaaccttac caaccactca gaaaggaaca gcgaaagtca
     4681 ttccgggtac gggggttaga attaactatc aagattattg gacagatgaa ttttatcgtg
     4741 ttgaaaatca atctgttcct gtccgttatg acccccttga ttatggcgta gcttatgcct
     4801 atattaacaa tcattgggtg aagtgtttat ctaattatca tctcagattt caaggttatt
     4861 cagaaaaagc tgtagccatt gcgacaacga tttgccgaca aaagcgaaaa cagtccaatc
     4921 aaagtctata tgtcggcgca aatgagcttg ttagtttact caaaaatgcg gaagactatg
     4981 aagaattaat gttacaaatt cgcaaagatc aatcagcaaa attagtctat aatttaatag
     5041 aaggaaattt aagcagtgcg gttttaaatc cagccgatat tgagcatcct tttttacagg
     5101 atgatagcaa agttgataac aatttttcta atttagacga agatatttta caacccagtt
     5161 tatcaacaac aactcaatca gataacgagg atgacgacat cggattttat agcaatgagg
     5221 aattatggta atgactgaaa aacgcctttt ttcttgggaa ttgttagaag actcaacgga
     5281 aaacaaatta aaatatttta aagatgtgat tattcctcat cgaaatatta aaactatttt
     5341 agagcagtta ctaaaaaatg cgattgaacc tgatgatgcg ttagtttatc tggtttttgg
     5401 ggtaacaggg gttggtaaat ctcgacttaa agcagaattt gagaagcgat tattgagaca
     5461 ctttgaagct gaaatcattg cgaatccagg tagtttggta gttgggggaa ttgaggtaga
     5521 tggtgcagaa ggaggtaagt ttaattattc cgattattat atccaagttt tagaatcttt
     5581 acaagaagtt ttgattgact ataaagaaaa ttatgagatt gatgaagacg ataatgaaga
     5641 taatttacga ggagatcatg aagggaaaaa ttctaaagca ttacgtcgaa caatgcaaaa
     5701 agtttttacc caccgtcagt taaaagctta tactttagat gaagcacagc atttatttga
     5761 tgtagcagga ggaagacaaa tcaatgttca gatgaattgg cttaaatcta ttgccaataa
     5821 aactcaaacg gttcatgttt tatttggaac ttatgacctt ttgaagtgtc aagaagtgaa
     5881 tggacaagta gggagaagaa gcgaagatta ttatctacct ccctattatt tggataattc
     5941 tgatgaccaa aaagaattta aaaaagcaat taatacttta caacgacatt ttcctgttga
     6001 aaatgaaccc gaattaaatg aaaagtggga atattttatg gaatattcca taggatgtat
     6061 cggtattctt aaaagttggt ggtatcgtgc gctcaaaaat gccttggatg atggtgcaaa
     6121 aacggtcaga atcaaggatt ttcaagcaag ggaattatct tctggaagac gaaaaacgat
     6181 taaaaaagag ttagaagacg gagaaagacg tttagctgcc ttatattttg aagagaaagc
     6241 caaaaagcct aaatctgagt ctcaagcttc atcaaaatca agcacaagaa aagtgggaga
     6301 acgtaaagcg attagggact tagtaggggt taatcaagag ggatgagcat ctatgaggag
     6361 ttttggagtt ctaaacccct aaaacttccc ccaaggagtc ggttattctg tcttaaacct
     6421 atgggagttg gaacttgcta tacggaaagt ctcagtagtt atgtgaatcg tttagcacag
     6481 gagcattgtt taacatcgca gaagttaata atggcagaaa ttgcgccact tattctaaaa
     6541 aatgaggata aatcggattt attaaagaaa agtgttagtc atttattggg taatacggat
     6601 gccaaaccag tgattaatgg aatgcgtgag atgacggggg aattagttag ggtgttagaa
     6661 cagttaacga tgcgtcagga tttacggttt ttgacgcttt taagctggaa aggaatgatt
     6721 tatgaccgcc agttatttaa aaattatcgt gcgtggtgtc cttgttgttt ggaggagtgg
     6781 aaaggggaga aaaaggtcat ctatgagccg ttattatggt catttaagga tgtagaagtg
     6841 tgtttacttc ataaacagcg attaattgag gaatgtcctc actgtaacgc tcgtttacct
     6901 gtatttgcaa ggttatcccc tgtggggttt tgttctcgtt gtcggggatg gttaggggat
     6961 aagaaagaag agatggaaga ggtggaaccc catcgagtaa atattgaggg aattggagat
     7021 ttaattgcgg taacgccgaa aatggcttac aaaccgattc cgattgagtt aacccgtaag
     7081 ttagaggtga ttttattggc gtttgaggaa gcctgtggga aggatatcac gttattgggg
     7141 gatttagggg taattatgga gcaaatgagg atagcaacga aacagcatca aaacaagcct
     7201 taccatctgg ttaaattggt tattcctgtt tgtgagaagg caaatattcg ggtatctgag
     7261 ttattattat tgaattttaa ggcgttaagc gatagggtgt ttgaaaattt ggagttaggg
     7321 atgaggcttt aactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
     7381 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     7441 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cgtaatacga
     7501 ctcactatag gggaattgtg agcggataac aattcccctc taggatccat cttagtatat
     7561 tagttaagta taagaaggag atatacatat ggacatagac ataaacaaaa ttcaacccta
     7621 taccaacgat gagctatgga gtccagagaa atttcccatg cctcctcgaa gtcgtttgca
     7681 tcatttacaa ccgatgggga taggaacccc aatggttgag agtttaacca gttatgtgac
     7741 tcgtttggct cattctcatg gggttcatgt gggaactcta atttataaag aaatcatccc
     7801 ttgtatttcc aaaacatata aaaccaccaa tttactttgc ttacgcaatc aaacaggagc
     7861 aatcaatggt tgtggtcttc ttgccgaaga ttcggttcaa gctttatcag aactcaccct
     7921 atatcaaaat ttatctaatt taactatgtt gaattggaaa aacatttttc caaacagaaa
     7981 attaatccga aaccataagg catggtgtcc attttgttat caagaatggc gaactaataa
     8041 tcaaattata tttgaccctt taatttggca aattgaagta attcgattat gttcaattca
     8101 tcaatattct ttacaaacac tttgtccttc ttgtaaaaag tctgtatcag tgttatcacc
     8161 taagaatcaa ttgggttatt gttctaattg ttctaattgg cttggaaaca ttgaagatta
     8221 tcttaatact tctttatcaa ttcataccaa atttaaagat aaaagctatt ttttatggat
     8281 tgcggattgt ttgggaaact tattatcttt tccttcaact attaaatctc cctcaaaaaa
     8341 acagatttct caaattattc aagctattat ccaccaatca acaaaaggta acattgcaga
     8401 atttgctaga tgggctaata tgccgaaaaa tacggtatgg atgtgggtta aaggtaaaag
     8461 cttacctcaa cttagtcgct tgttagcact atgctatttg tgtcgcgttg atttactaga
     8521 ttcaataatc aacgagggaa atatcacaaa catgattctt catcaaaaga gtattccttc
     8581 ttctgtaaat gaacgcccga aaatcttcaa gaacacttta aaccatgcgc aaattcatgc
     8641 tgctctctca gaagctttcc aagaaaccca tcctcgccca atgactcaag ttgctaaacg
     8701 attaggaatc agcaataaag tgctgcgaag acactttcct gagctttgta agaaaatttc
     8761 tcaacgttac ctcatttaca aaaaacaatc tcgcttggaa cgtattcaga aagtttgtat
     8821 agaagtagaa aaagcagtta aacaacttca acaacaaggt atttacccta gcgaacaacg
     8881 agttgcatta catatcggtc agcccggttg tttccgagat aaagaagtca gagaagtcct
     8941 aaaccgaact cgacgtaatt ggagattgtt agatacctga ctcgagcacc accaccacca
     9001 ctgattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9061 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9121 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9181 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9241 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9301 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9361 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9421 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     9481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9541 tgtatccgct catgaattaa ttcttagaaa aactcatcga gcatcaaatg aaactgcaat
     9601 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     9661 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     9721 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     9781 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct
     9841 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     9901 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa
     9961 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
    10021 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
    10081 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
    10141 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
    10201 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
    10261 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
    10321 tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata
    10381 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga ccaaaatccc
    10441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    10501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10621 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
    10681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    11041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    11101 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    11161 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    11221 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
    11281 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
    11341 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
    11401 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
    11461 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    11521 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
    11581 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc c
//
