LOCUS       sequence-354973-       13590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-induced clustering of EGFR cytosolic domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13590)
  AUTHORS   Farahani PE, Lemke SB, Dine E, Uribe G, Toettcher JE, Nelson CM
  TITLE     Substratum stiffness regulates Erk signaling dynamics through 
            receptor-level control.
  JOURNAL   Cell Rep. 2021 Dec 28;37(13):110181. doi: 
            10.1016/j.celrep.2021.110181.
  PUBMED    34965432
REFERENCE   2  (bases 1 to 13590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-12-28"; volume: "37(13):110181. doi"; pages: " 
            10.1016/j.celrep.2021.110181"
COMMENT     Sequence Label: MYR-FR-C2D-EGFR
FEATURES             Location/Qualifiers
     source          1..13590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             754..774
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             1179..1190
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1450..2145
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             2164..3657
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    5447..6035
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5500..5520)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5918..5929)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6038..6054)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6039..6055)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6145..6378
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6403..6421)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6404..6421)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6711..6730)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6944..6963)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7063..7085
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7123..7141)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7209..7313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7314..7382,7383..8174)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8345..8933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8834..8853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9087..9104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9174..9194)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9179..9508
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9359..9494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10642..10707
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10837..10857
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11282..11416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11319..11338)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11373..11392
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11584..12217
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12264..12389
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12886..13119
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13304..13348
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtatgggtt cctccaaatc caagcccaag
      781 gacgcatctc agcgacgtcg cgcctcaaac gattataccc aacaagcaac ccaaagctat
      841 ggggcctacc ccacccagcc cgggcagggc tattcccagc agagcagtca gccctacgga
      901 cagcagagtt acagtggtta tagccagtcc acggacactt caggctatgg ccagagcagc
      961 tattcttctt atggccagag ccagaacaca ggctatggaa ctcagtcaac tccccaggga
     1021 tatggctcga ctggcggcta tggcagtagc cagagctccc aatcgtctta cgggcagcag
     1081 tcctcctatc ctggctatgg ccagcagcca gctcccagca gcacctcggg aagttacggt
     1141 agcagttctc agagcagcag ctatgggcag ccccagagtg ggagctacag ccagcagcct
     1201 agctatggtg gacagcagca aagctatgga cagcagcaaa gctataatcc ccctcagggc
     1261 tatggacagc agaaccagta caacagcagc agtggtggtg gaggtggagg tggaggtgga
     1321 ggtaactatg gccaagatca atcctccatg agtagtggtg gtggcagtgg tggcggttat
     1381 ggcaatcaag accagagtgg tggaggtggc agcggtggct atggacagca ggaccgtgga
     1441 ggtggaggaa tggtgagcga gctgattaag gagaacatgc ccatgaagct gtacatggag
     1501 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     1561 ggcacccaga ccatgagaat caaggtcgtc gagggcggcc ctctcccctt cgccttcgac
     1621 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaagcaccc tccgggcatc
     1681 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     1741 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     1801 tacaacgtca aggttagagg ggtgaacttc ccagccaacg gccctgtgat gcagaagaaa
     1861 acactcggct gggaggcctc caccgagacg atgtaccccg ctgacggcgg cctggaaggc
     1921 gcatgtgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa ccttgagacc
     1981 acatacagat ccaagaaacc cgctacgaac ctcaagatgc ccggcgtcta caacgtggac
     2041 cacagactgg aaagaatcaa ggaggccgac gatgagacct acgtcgagca gcacgaggtg
     2101 gctgtggcca gatactctac tggtggcgct ggtgatggag gtaaagcggc cacgcgtacg
     2161 cgtatgaaga tggacaaaaa gactatagtt tggtttagaa gagacctaag gattgaggat
     2221 aatcctgcat tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt catttggtgt
     2281 cctgaagaag aaggacagtt ttatcctgga agagcttcaa gatggtggat gaaacaatca
     2341 cttgctcact tatctcaatc cttgaaggct cttggatctg acctcacttt aatcaaaacc
     2401 cacaacacga tttcagcgat cttggattgt atccgcgtta ccggtgctac aaaagtcgtc
     2461 tttaaccacc tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa ggagaagctg
     2521 gtggaacgtg ggatctctgt gcaaagctac aatggagatc tattgtatga accgtgggag
     2581 atatactgcg aaaagggcaa accttttacg agtttcaatt cttactggaa gaaatgctta
     2641 gatatgtcga ttgaatccgt tatgcttcct cctccttggc ggttgatgcc aataactgca
     2701 gcggctgaag cgatttgggc gtgttcgatt gaagaactag ggctggagaa tgaggccgag
     2761 aaaccgagca atgcgttgtt aactagagct tggtctccag gatggagcaa tgctgataag
     2821 ttactaaatg agttcatcga gaagcagttg atagattatg caaagaacag caagaaagtt
     2881 gttgggaatt ctacttcact actttctccg tatctccatt tcggggaaat aagcgtcaga
     2941 cacgttttcc agtgtgcccg gatgaaacaa attatatggg caagagataa gaacagtgaa
     3001 ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt taagagagta ttctcggtat
     3061 atatgtttca acttcccgtt tactcacgag caatcgttgt tgagtcatct tcggtttttc
     3121 ccttgggatg ctgatgttga taagttcaag gcctggagac aaggcaggac cggttatccg
     3181 ttggtggatg ccggaatgag agagctttgg gctaccggat ggatgcataa cagaataaga
     3241 gtgattgttt caagctttgc tgtgaagttt cttctccttc catggaaatg gggaatgaag
     3301 tatttctggg atacactttt ggatgctgat ttggaatgtg acatccttgg ctggcagtat
     3361 atctctggga gtatccccga tggccacgag cttgatcgct tggacaatcc cgcgttacaa
     3421 ggcgccaaat atgacccaga aggtgagtac ataaggcaat ggcttcccga gcttgcgaga
     3481 ttgccaactg aatggatcca tcatccatgg gacgctcctt taaccgtact caaagcttct
     3541 ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag acatcgacac agctcgtgag
     3601 ctactagcta aagctatttc aagaacccgt gaagcacaga tcatgatcgg agcagcagcc
     3661 cgggatccac cggtcgccac cggttcaggg agcggatccg gctcagggag tgcggccgca
     3721 cgaaggcgcc acatcgttcg gaagcgcacg ctgcggaggc tgctgcagga gagggagctt
     3781 gtggagcctc ttacacccag tggagaagct cccaaccaag ctctcttgag gatcttgaag
     3841 gaaactgaat tcaaaaagat caaagtgctg ggctccggtg cgttcggcac ggtgtataag
     3901 ggactctgga tcccagaagg tgagaaagtt aaaattcccg tcgctatcaa ggaattaaga
     3961 gaagcaacat ctccgaaagc caacaaggaa atcctcgatg aagcctacgt gatggccagc
     4021 gtggacaacc cccacgtgtg ccgcctgctg ggcatctgcc tcacctccac cgtgcaactc
     4081 atcacgcagc tcatgccctt cggctgcctc ctggactatg tccgggaaca caaagacaat
     4141 attggctccc agtacctgct caactggtgt gtgcagatcg caaagggcat gaactacttg
     4201 gaggaccgtc gcttggtgca ccgcgacctg gcagccagga acgtactggt gaaaacaccg
     4261 cagcatgtca agatcacaga ttttgggctg gccaaactgc tgggtgcgga agagaaagaa
     4321 taccatgcag aaggaggcaa agtgcctatc aagtggatgg cattggaatc aattttacac
     4381 agaatctata cccaccagag tgatgtctgg agctacgggg tgaccgtttg ggagttgatg
     4441 acctttggat ccaagccata tgacggaatc cctgccagcg agatctcctc catcctggag
     4501 aaaggagaac gcctccctca gccacccata tgtaccatcg atgtctacat gatcatggtc
     4561 aagtgctgga tgatagacgc agatagtcgc ccaaagttcc gtgagttgat catcgaattc
     4621 tccaaaatgg ccagagaccc ccagcgctac cttgtcattc agggggatga aagaatgcat
     4681 ttgccaagtc ctacagactc caacttctac cgtgccctga tggatgaaga agacatggac
     4741 gacgtggtgg atgccgacga gtacctcatc ccacagcagg gcttcttcag cagcccctcc
     4801 acgtcacgga ctcccctcct gagctctctg agtgcaacca gcaacaattc caccgtggct
     4861 tgcattgata gaaatgggct gcaaagctgt cccatcaagg aagacagctt cttgcagcga
     4921 tacagctcag accccacagg cgccttgact gaggacagca tagacgacac cttcctccca
     4981 gtgcctgaat acataaacca gtccgttccc aaaaggcccg ctggctctgt gcagaatcct
     5041 gtctatcaca atcagcctct gaaccccgcg cccagcagag acccacacta ccaggacccc
     5101 cacagcactg cagtgggcaa ccccgagtat ctcaacactg tccagcccac ctgtgtcaac
     5161 agcacattcg acagccctgc ccactgggcc cagaaaggca gccaccaaat tagcctggac
     5221 aaccctgact accagcagga cttctttccc aaggaagcca agccaaatgg catctttaag
     5281 ggctccacag ctgaaaatgc agaataccta agggtcgcgc cacaaagcag tgaatttatt
     5341 ggagcatgag cggccgcaac tcccacctgc aacatgcgtg actgactgag gccgcgactc
     5401 tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga
     5461 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5521 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5581 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5641 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5701 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5761 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5821 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     5881 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5941 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6001 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa
     6061 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6121 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6181 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6241 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6301 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6361 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
     6421 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
     6481 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
     6541 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
     6601 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
     6661 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     6721 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     6781 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     6841 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     6901 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     6961 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7021 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7081 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7141 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7201 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7261 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7321 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7381 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7441 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7501 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7561 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7621 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7681 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7741 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7801 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7861 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7921 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7981 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8041 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8101 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8161 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8221 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8281 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8341 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8401 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8461 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8521 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8581 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8641 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8701 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8761 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8821 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8881 gacttgagcg tcgatttttg tgatgctcgc caggggggcg gagcctatgg aaaaacgcca
     8941 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9001 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9061 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9121 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     9181 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9241 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9301 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9361 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9421 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9481 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     9541 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     9601 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     9661 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     9721 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     9781 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     9841 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     9901 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     9961 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
    10021 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
    10081 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
    10141 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
    10201 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
    10261 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
    10321 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
    10381 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
    10441 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
    10501 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    10561 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
    10621 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
    10681 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
    10741 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
    10801 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    10861 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    10921 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    10981 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
    11041 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
    11101 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    11161 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    11221 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    11281 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11341 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11401 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11461 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    11521 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    11581 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
    11641 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
    11701 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
    11761 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
    11821 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
    11881 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
    11941 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
    12001 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
    12061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    12121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    12181 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
    12241 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
    12301 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
    12361 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
    12421 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
    12481 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
    12541 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
    12601 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
    12661 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
    12721 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    12781 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    12841 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    12901 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    12961 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    13021 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    13081 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    13141 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    13201 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    13261 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    13321 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    13381 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    13441 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    13501 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    13561 agacagagac agatccattc gattagtgaa
//
