LOCUS       sequence-354976-       15088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2819
FEATURES             Location/Qualifiers
     source          1..15088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8026..8614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8079..8099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8497..8508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8686..8919
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8997..9118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9034..9053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9088..9107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9158..9293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9313..9332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9314..9332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9342..9359)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9342..9358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9351..9373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9500..9955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9587..9606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9797..9818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9981..10085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10086..10154,10155..10946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10304..10323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11117..11705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11606..11625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11859..11876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11993..12014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12029..12034,12035..12052,12053..12059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12067..12083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12072..12094
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12091..12107
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12091..12107
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12126..12146
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12128..12146
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12174..12400
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12401..12581
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12628..12753
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13246..13479
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13664..13708
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        13939..14149
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        13961..14157
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      14008..14143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             14189..14791
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(14189..14208)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     14685..14705
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    14834..14951
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtt
     1201 gccctgtaag aagagaagaa ctacagtgac agagtcccta cagcataaag gcaatcaaga
     1261 ggaaaacaac gtagacctag aatcagccgt taaaccagaa tctgaccagg ttaaggactt
     1321 gagttcggtg tcactatcct gggatccaag tcatggcaga gtagctggct tcgaagtaca
     1381 gtctttgcag gatgcaggaa atcagcttgg tatggaggat acatctctga gctctggaat
     1441 gctcacccag aacacaaatg taccaattct agaaggtgtt gatgtggcca tctctcaggg
     1501 aatcacccta ccttccttgg agtcttttca cccccttaat atacacattg gtaaaggaaa
     1561 actccacgct actggctcaa agagagggaa aaaaatgaca ctcaggcctg ggccagttac
     1621 ccaagaagac agatgtgatc atcttaccct aaaggagcct ttttcaggag agcctagtga
     1681 agaagtcaag gaagaaggag ggaaacctca aatgaattct gaaggggaga taccttccct
     1741 gccatcaggc agccaatctg caaaaccagt aagccagccc aggaaatcaa cccagccaga
     1801 tgtttgtgcc tctcctcaag aaaagccact caggactctg tttcaccaac ctgaggaaga
     1861 gatagaagat ggtggactct tcattccaat ggaagaacaa gacaatgaag aaagtgagaa
     1921 aaggagaaaa aagaaaaagg gtaccaagag gaaacgagat ggaaggggtc aagaagggac
     1981 cttggcatat gacctgaaac tggatgacat gcttgaccgt accttggagg atggtgccaa
     2041 gcagcacaat ctaacagcag tcaatgtccg aaacatcctt catgaagtaa tcacaaatga
     2101 acacgtggta gctatgatga aagcagccat cagtgagacg gaagatatgc caatgtttga
     2161 gcctaaaatg acacgctcta aactgaagga agtagtggaa aaaggagtgg taattccaac
     2221 atggaatatt tcaccaatta agaaggccaa tgaaattaag cctcctcagt ttgtggatat
     2281 ccaccttgaa gaagatgatt cctcagatga agaataccag ccggatgatg aagaagaaga
     2341 tgaaactgct gaagagagct tattggaaag tgatgttgaa agcactgctt catctccacg
     2401 tggggcaaag aaatccagat tgaggcagtc ttctgagatg actgaaacag atgaggagag
     2461 tggcatatta tcagaggctg agaaagtcac cacaccagcc atcaggcaca tcagtgctga
     2521 ggtagtgccc atggggcccc cgccccctcc aaagccgaaa cagaccagag atagtacttt
     2581 catggagaag ttacatgcgg tagatgagga gctggcttcc agtccagtct gcatggattc
     2641 tttccagccc atggatgaca gtctcattgc atttcgaacg cgttctaaga tgcccctgaa
     2701 agatgttccc ctgggccaat tagaggcaga gctccaagct ccagacatca ctccagatat
     2761 gtatgacccc aatacggcag atgatgagga ctggaagatg tggctggggg gacttatgaa
     2821 tgatgatgtg gggaatgaag atgaagcaga tgatgatgat gatccagaat ataatttcct
     2881 ggaagacctc gatgaaccag acacagagga tttccggact gaccgggcag tgagaatcac
     2941 caaaaaggaa gtaaatgagc tgatggaaga gctgtttgaa actttccaag atgagatggg
     3001 attctccaac atggaagatg atggcccaga agaggaggag tgtgtagctg agcctcgtcc
     3061 taactttaac acccctcaag ctctacggtt tgaggaacca ctggccaacc tgttaaatga
     3121 acaacatcgg acagtgaagg agctatttga acagctgaag atgaagaaat cttcagccaa
     3181 acagctgcag gaagtagaga aggttaaacc ccagagtgag aaagttcatc agactctgat
     3241 tctggaccca gcacagagga agagactcca gcagcagatg cagcagcacg ttcagctctt
     3301 gacccaaatc caccttcttg ccacctgcaa ccccaacctc aatccggagg ccactaccac
     3361 caggatattt cttaaagagc tgggaacctt tgctcaaagc tccatcgccc ttcaccatca
     3421 gtacaacccc aagtttcaga ccctgttcca accctgtaac ttgatgggag ctatgcagct
     3481 gattgaagac ttcagcacac atgtcagcat tgactgcagc cctcataaaa ctgtcaagaa
     3541 gactgcgaat gaatttccct gtttgccaaa gcaagtggct tggattctgg ccacaagcaa
     3601 ggttttcatg tatccagagt tacttccagt gtgttccctg aaggcaaaga atccccagga
     3661 taagatcgtc ttcaccaagg ctgaggacaa tttgttagct ttaggactga agcattttga
     3721 aggaactgag tttcctaatc ctctaatcag caagtacctt ctaacctgca aaactgccca
     3781 ccaactgaca gtgagaatca agaacctcaa catgaacaga gctcctgaca acatcattaa
     3841 attttataag aagaccaaac agctgccagt cctaggaaaa tgctgtgaag agatccagcc
     3901 acatcagtgg aagccaccta tagagagaga agaacaccgg ctcccattct ggttaaaggc
     3961 cagtctgcca tccatccagg aagaactgcg gcacatggct gatggtgcta gagaggtagg
     4021 aaatatgact ggaaccactg agatcaactc agatcgaagc ctagaaaaag acaatttgga
     4081 gttggggagt gaatctcggt acccactgct attgcctaag ggtgtagtcc tgaaactgaa
     4141 gccagttgcc acccgtttcc ccaggaaggc ttggagacag aagcgttcat cagtcctgaa
     4201 gcccctcctt atccaaccca gcccctctct ccagcccagc ttcaaccctg ggaaaacacc
     4261 agcccgatca actcattcag aagcccctcc gagcaaaatg gtgctccgga ttcctcaccc
     4321 aatacagcca gccactgttt tacagacagt tccaggtgtc cctccactgg gggtcagtgg
     4381 aggtgagagt tttgagtctc ctgcagcact gcctgctgtg ccccctgagg ccaggacaag
     4441 cttccctctg tctgagtccc agactttgct ctcttctgcc cctgtgccca aggtaatgct
     4501 gccctccctt gccccttcta agtttcgaaa gccatatgtg agacggagac cctcaaagag
     4561 aagaggagtc aaggcctctc cctgtatgaa acctgcccct gttatccacc accctgcatc
     4621 tgttatcttc actgttcctg ctaccactgt gaagattgtg agccttggcg gtggctgtaa
     4681 catgatccag cctgtcaatg cggctgtggc ccagagtccc cagactattc ccatcactac
     4741 cctcttggtt aaccctactt ccttcccctg tccattgaac cagtcccttg tggcctcctc
     4801 tgtctcaccc ttaattgttt ctggcaattc tgtgaatctt cctataccat ccacccctga
     4861 agataaggcc cacgtgaatg tggacattgc ttgtgctgtg gctgatgggg aaaatgcctt
     4921 tcagggccta gaacccaaat tagagcccca ggaactatct cctctctctg ctactgtttt
     4981 cccgaaagtg gaacatagcc cagggcctcc actagcagat gcagagtgcc aagaaggatt
     5041 gtcagagaat agtgcctgtc gctggaccgt tgtgaaaaca gaggagggga ggcaagctct
     5101 ggagccgctc cctcagggca tccaggagtc tctaaacaac cctacccctg gggatttaga
     5161 ggaaattgtc aagatggaac ctgaagaagc tagagaggaa atcagtggat cccctgagcg
     5221 tgatatttgt gatgacatca aagtggaaca tgctgtggaa ttggacactg gtgccccaag
     5281 cgaggagttg agcagtgctg gagaagtaac gaaacagaca gtcttacaga aggaagagga
     5341 gaggagtcag ccaactaaaa ccccttcatc ttctcaagag ccccctgatg aaggaacctc
     5401 agggacagat gtgaacaaag gatcatcaaa gaatgctttg tcctcaatgg atcctgaagt
     5461 gaggcttagt agccccccag ggaagccaga agattcatcc agtgttgatg gtcagtcagt
     5521 ggggactcca gttgggccag aaactggagg agagaagaat gggccagaag aagaggaaga
     5581 agaggacttt gatgacctca cccaagatga ggaagatgaa atgtcatcag cttctgagga
     5641 atctgtgctt tctgtcccag aactccagga aacaatggag aaactgactt ggctggcatc
     5701 tgaaaggcgc atgagtcagg agggtgagtc tgaagaagag aattctcagg aggagaactc
     5761 tgagccagaa gaagaggagg aagaagaagc agaaggaatg gaaagcctgc agaaagagga
     5821 tgaaatgacg gatgaagcag ttggagactc tgctgagaag cctcctactt ttgcttcacc
     5881 tgagactgct ccagaagtgg agaccagcag aactccacca ggagagagca tcaaagctgc
     5941 tggaaaaggc cggaacaatc atcgagctcg caacaagcgg ggaagtcggg ctcgggccag
     6001 caaggacacc tccaagctgc tgttgctgta tgatgaggac attctcgagc gagatccact
     6061 cagggagcag aaggacttgg cctttgccca agcttatctg accagggtgc gagaagccct
     6121 acaacatatc cctggcaagt atgaagactt ccttcaagtc atctatgaat ttgagtcaag
     6181 tacccagaga cggacggctg tagatctcta caaaagcctg caaattctgc tccaagactg
     6241 gcctcagctg ttgaaagact ttgctgcttt cctgttacct gagcaagctc tggcctgtgg
     6301 attatttgag gagcagcagg cttttgagaa gagccgcaag ttccttcggc agctggagat
     6361 ttgctttgca gagaacccct cacaccacca gaagattatc aaggtcctcc aaggctgtgc
     6421 agactgcctt ccccaggaga tcaccgagct caagacacag atgtggcagc tcctcaaggg
     6481 ccacgaccac ctgcaggatg agttttctat cttctttgac cacttgcgcc cagcagctag
     6541 ccggatgggt gactttgaag agatcaattg gactgaggaa aaggagtatg agtttgatgg
     6601 ctttgaagaa gtggccctgc ctgatgtgga agaagaggag gagcctccca agatacccac
     6661 agcctcaaag aacaagagga aaaaagagat cggggtccaa aatcatgata aggagactga
     6721 atggccagat ggggccaagg actgtgcctg ctcctgccat gaaggaggtc cagattccaa
     6781 gctgaagaag agcaaaaggc ggagctgtag ccactgtagc agcaaggtct gtgacagcaa
     6841 atcctacaag agcaaggagc cccatgagtt ggtgggcagc agcccccacc gagaggctag
     6901 tcctatgcct ggtgctaagg aagctgggca gggcaaggat atgatggaag aggaagcccc
     6961 agaggagcgg gagagcactg aggccaccca gagcaggact gtcaggacca ccagaaaggg
     7021 agagatgcct gtttcaggat tggcagtggg gagcactttg ccatcccctc gagaagtgac
     7081 tgttacagaa cggctcctcc tggatggccc accacctcat tcaccagaga ctcctcaatt
     7141 tccccccaca actggagctg tactgtacac tgttaagaga aaccaggttg ggcctgaggt
     7201 tcgctcctgc cccaaggcat cccccagact tcagaaagag agggagggcc aaaaggcagt
     7261 gagtgagtca gaggctttga tgctggtctg ggatgcatca gaaactgaga aattgcctgg
     7321 taccgtggaa ccccctgctt ccttcctgag tcctgtttcc tcaaagacca gagatgcagg
     7381 gagaagacat gtgtccggga aaccagacac tcaagagaga tggctgccct caagcagagc
     7441 tcgggtgaag acaagagaca ggacgtgccc tgtccatgaa tctccatcag gaattgacac
     7501 ctcagagact tctcccaaag cccctagagg gggtttggct aaagacagtg gaacacaggc
     7561 caagggtcca gagggggagc agcagccaaa ggccgcagaa gctacggtgt gtgccaacaa
     7621 cagcaaggtc agctccactg gggaaaaggt tgtcctgtgg acaagggaag ctgaccgtgt
     7681 gatcctcacc atgtgccagg agcaaggggc acagccacag accttcaaca tcatctccca
     7741 gcagctggga aataagaccc ctgctgaggt ttcccaccgt tttcgagaac tcatgcagct
     7801 cttccacact gcctgtgaag ccagctctga ggatgaggat gatgcaacca gtaccagcaa
     7861 tgcagaccag ctgtctgacc atggggacct tctgtctgaa gaggagctgg atgaatagta
     7921 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     7981 ggacgacatc cttctgaatg tcagatttaa acgcgttaag tcgacaatca acctctggat
     8041 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8101 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8161 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8221 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8281 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8341 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8401 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8461 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8521 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8581 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     8641 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8701 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8761 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8821 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8881 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     8941 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     9001 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9061 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9121 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9241 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9301 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9361 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9421 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9481 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9541 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9601 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9661 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9721 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9781 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9841 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9901 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9961 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10081 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10201 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10261 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10321 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10381 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10441 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10501 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10561 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10621 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10681 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10741 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10801 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10861 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10921 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10981 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11041 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    11281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11821 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11881 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11941 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    12001 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    12061 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    12121 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
    12181 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
    12241 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
    12301 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
    12361 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
    12421 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    12481 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    12541 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    12601 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    12661 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    12721 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    12781 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    12841 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    12901 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    12961 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    13021 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    13081 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    13141 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    13201 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    13261 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    13321 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    13381 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    13441 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    13501 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    13561 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    13621 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    13681 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    13741 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    13801 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    13861 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
    13921 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
    13981 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    14041 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    14101 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    14161 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
    14221 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
    14281 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
    14341 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
    14401 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
    14461 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    14521 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    14581 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    14641 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    14701 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    14761 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
    14821 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    14881 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    14941 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    15001 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    15061 cttctaggtc ttgaaaggag tgggaatt
//
