LOCUS       sequence-354977-        2501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pC7
FEATURES             Location/Qualifiers
     source          1..2501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(79..667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(159..178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     973..989
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     973..989
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     994..1013
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        994..1012
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1187..2377)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2006..2025
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2425..2430,2431..2447,2448..2453))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
       61 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      121 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      181 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      241 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      301 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      361 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      421 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      481 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      541 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      601 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      661 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgta
      721 actcacgtta agggattttg gtcatgggtg gctcgaccag atatggtctc aacttgcaat
      781 cgctacaact tgcgctgggg ttagtccgtg ggcttgacag agaactggta gaaggcgttg
      841 gacggtttca agagcctgtt tgccaccgtc atgactggct cgagaccagc tttggtctag
      901 aggaggcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
      961 ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata ggggatattt
     1021 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     1081 ttccccgatc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
     1141 attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg
     1201 cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta
     1261 caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca
     1321 gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct
     1381 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
     1441 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca
     1501 agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac
     1561 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg
     1621 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc
     1681 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
     1741 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
     1801 gcatgggtcg gcgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     1861 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg taaggagatg gcgcccaaca
     1921 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     1981 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     2041 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gagaatccac aggacgggtg
     2101 tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc
     2161 ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
     2221 catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
     2281 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     2341 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     2401 aatttaactg tgataaacta ccgcattaaa gctaatcgat gataagctgt caaacatgag
     2461 aattaattca tgttctttcc tgcgttatcc cctgattctg t
//
