LOCUS       sequence-354978-        2498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pC8
FEATURES             Location/Qualifiers
     source          1..2498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     458..477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(684..689,690..706,707..712)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             760..1950
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1112..1131)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2124..2143)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2125..2143)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2148..2164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2148..2164)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2467..2498,1..557)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
       61 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      361 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      541 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      601 ggccttttgc tcacatgttc tttcctgcgt tatccacaga atcaggggat aacgcaggaa
      661 agaacatgaa ttaattctca tgtttgacag cttatcatcg attagcttta atgcggtagt
      721 ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc
      781 atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta
      841 ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg
      901 ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc
      961 gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac
     1021 gcgatcatgg cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggccggc
     1081 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     1141 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     1201 cccgtggccg ggggactgtt gggcgccatc tccttacatg caccattcct tgcggcggcg
     1261 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     1321 gagcgccgac ccatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     1381 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     1441 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     1501 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     1561 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
     1621 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
     1681 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     1741 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact
     1801 tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac
     1861 gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc
     1921 ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat
     1981 tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
     2041 acccttgatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     2101 tgattgggag aaaataaaat atcccctata gtgagtcgta ttacatggtc atagctgttt
     2161 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2221 tatcatcatg cctcctctag accaaagctg gtctcaggaa gcaatcgcta caacttgcgc
     2281 tggggttagt ccgtgggctt gacagagaac tggtagaagg cgttggacgg tttcaagagc
     2341 ctgtttgcca ccgtcatgac ttcgagacca tatctggtcg agccacccat gaccaaaatc
     2401 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2461 atcttcttga gatccttttt ttctgcgcgt aatctgct
//
