LOCUS       sequence-354980-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CC2
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     426..442
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     426..442
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     447..466
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        447..465
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(640..1830)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1459..1478
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1878..1883,1884..1900,1901..1906))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2033..2604,1..17))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2113..2132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
       61 acgacgcgta actcacgtta agggattttg gtcatgggtg gctcgaccag atatggtctc
      121 gcaggttgta gcgattgcta gtcatgacgg tggcaaacag gctcttgaaa ccgtccaacg
      181 ccttctacca gttctctgtc aagcccacgg actaacccca gcgcaagttg tagcgattgc
      241 tagtcatgac ggtggcaaac aggctcttga aaccgtccaa cgccttctac cagttctctg
      301 tcaagcccac ggactaaccc cagcgcaagt ggttgagacc agctttggtc tagaggaggc
      361 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acgggccaga
      421 gctgccagga aacagctatg accatgtaat acgactcact ataggggata ttttattttc
      481 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
      541 atcaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
      601 gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
      661 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca
      721 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc
      781 aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc
      841 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
      901 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta
      961 gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
     1021 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
     1081 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
     1141 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat
     1201 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatggg
     1261 tcggcgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     1321 gttgagcacc gccgccgcaa ggaatggtgc atgtaaggag atggcgccca acagtccccc
     1381 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     1441 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     1501 gccggtgatg ccggccacga tgcgtccggc gtagagaatc cacaggacgg gtgtggtcgc
     1561 catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc
     1621 aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag
     1681 cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag
     1741 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
     1801 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
     1861 ctgtgataaa ctaccgcatt aaagctaatc gatgataagc tgtcaaacat gagaattaat
     1921 tcatgttctt tcctgcgtta tcccctgatt ctgtggataa cgcaggaaag aacatgtgag
     1981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2461 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2581 tttgcaagca gcagattacg cgca
//
