LOCUS       sequence-354986-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CC5
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     359..375
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     359..375
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     380..399
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        380..398
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(573..1763)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1392..1411
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1811..1816,1817..1833,1834..1839))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1966..2554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2046..2065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgtaactca cgttaaggga ttttggtcat gggtggctcg accagatatg gtctcggcaa
       61 ttgctagtca tgacggtggc aaacaggctc ttgaaaccgt ccaacgcctt ctaccagttc
      121 tctgtcaagc ccacggacta accccagcgc aagttgtagc gattgctagt catgacggtg
      181 gcaaacaggc tcttgaaacc gtccaacgcc ttctaccagt tctctgtcaa gcccacggac
      241 taaccccagc gcaagttgta gcgatttgag accagctttg gtctagagga ggcatgatga
      301 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgcca
      361 ggaaacagct atgaccatgt aatacgactc actatagggg atattttatt ttctcccaat
      421 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatcaagg
      481 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
      541 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
      601 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac
      661 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccaatgatc
      721 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct
      781 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
      841 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
      901 gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
      961 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     1021 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     1081 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg
     1141 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat gggtcggcgc
     1201 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     1261 accgccgccg caaggaatgg tgcatgtaag gagatggcgc ccaacagtcc cccggccacg
     1321 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     1381 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     1441 atgccggcca cgatgcgtcc ggcgtagaga atccacagga cgggtgtggt cgccatgatc
     1501 gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg
     1561 tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc
     1621 agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc
     1681 agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg
     1741 atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat
     1801 aaactaccgc attaaagcta atcgatgata agctgtcaaa catgagaatt aattcatgtt
     1861 ctttcctgcg ttatcccctg attctgtgga taacgcagga aagaacatgt gagcaaaagg
     1921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2401 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2581 gtctgacgct cagtggaacg ac
//
