LOCUS       sequence-354989-        3084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CC7
FEATURES             Location/Qualifiers
     source          1..3084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(241..259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(264..280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      688..1276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1177..1196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1403..1408,1409..1425,1426..1431)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1479..2669
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1831..1850)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(2844..2862)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2867..2883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
       61 acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
      121 atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg aagaacagta
      181 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatcc
      241 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
      301 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
      361 aagctggtct cgagccagtc atgacggtgg caaacaggct cttgaaaccg tccaacgcct
      421 tctaccagtt ctctgtcaag cccacggact aaccccagcg caagttgtag cgattgctag
      481 tcatgacggt ggcaaacagg ctcttgaaac cgtccaacgc cttctaccag ttctctgtca
      541 agcccacgga ctaaccccag cgcaagttgt agcgattgca agttgagacc atatctggtc
      601 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
      661 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      721 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      781 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      841 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      901 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      961 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1021 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1081 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1141 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1201 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1261 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1321 gccttttgct cacatgttct ttcctgcgtt atccacagaa tcaggggata acgcaggaaa
     1381 gaacatgaat taattctcat gtttgacagc ttatcatcga ttagctttaa tgcggtagtt
     1441 tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca
     1501 tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac
     1561 tgccgggcct cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc
     1621 tgctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg
     1681 accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg
     1741 cgatcatggc gaccacaccc gtcctgtgga ttctctacgc cggacgcatc gtggccggca
     1801 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     1861 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     1921 ccgtggccgg gggactgttg ggcgccatct ccttacatgc accattcctt gcggcggcgg
     1981 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     2041 agcgccgacc catgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     2101 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     2161 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     2221 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     2281 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     2341 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     2401 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     2461 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     2521 cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     2581 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     2641 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
     2701 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
     2761 cccttgatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
     2821 gattgggaga aaataaaata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2881 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2941 atcatcatgc ctcctctaga ccaaagctgg tctcgagcca gtcatgacgg tggcaaacag
     3001 gctcttgaaa ccgtccaacg ccttctacca gttctctgtc aagcccacgg actaacccca
     3061 gcgcaagttg tagcgattgc tagt
//
