LOCUS       sequence-354992-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CC9
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      336..924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     825..844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1051..1056,1057..1073,1074..1079)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1127..2317
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1479..1498)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2491..2510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2492..2510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2515..2531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2515..2531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cctctagacc aaagctggtc tcaagaggca atcgctacaa cttgcgctgg ggttagtccg
       61 tgggcttgac agagaactgg tagaaggcgt tggacggttt caagagcctg tttgccaccg
      121 tcatgactag caatcgctac aacttgcgct ggggttagtc cgtgggcttg acagagaact
      181 ggtagaaggc gttggacggt ttcaagagcc tgtttgccac cgtcatggga acgagaccat
      241 atctggtcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
      301 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      361 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      421 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      481 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      541 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      601 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      661 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      721 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      781 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      841 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      901 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      961 ttttgctggc cttttgctca catgttcttt cctgcgttat ccacagaatc aggggataac
     1021 gcaggaaaga acatgaatta attctcatgt ttgacagctt atcatcgatt agctttaatg
     1081 cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa
     1141 tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat
     1201 gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta
     1261 tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc
     1321 actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat
     1381 cgactacgcg atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt
     1441 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     1501 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     1561 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttacatgcac cattccttgc
     1621 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     1681 taagggagag cgccgaccca tgcccttgag agccttcaac ccagtcagct ccttccggtg
     1741 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     1801 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     1861 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     1921 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     1981 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     2041 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     2101 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     2161 cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     2221 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
     2281 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
     2341 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
     2401 caaaccaacc cttgatcggg gaagaacagt atgtcgagct attttttgac ttactgggga
     2461 tcaagcctga ttgggagaaa ataaaatatc ccctatagtg agtcgtatta catggtcata
     2521 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2581 aaaaatatat catcatgcct
//
