LOCUS       sequence-355006-       11693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 
            3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be 
            integrated with pBac..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Kandul NP, Liu J, Buchman A, Shriner IC, Corder RM, Warsinger-Pepe 
            N, Yang T, Yadav AK, Scott MJ, Marshall JM, Akbari OS
  TITLE     Precision Guided Sterile Males Suppress Populations of an Invasive 
            Crop Pest
  JOURNAL   GEN Biotechnology (2022) 1:4, 372-385
REFERENCE   2  (bases 1 to 11693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GEN 
            Biotechnology (2022) 1:4, 372-385"
COMMENT     Sequence Label: 1161F
FEATURES             Location/Qualifiers
     source          1..11693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   624..686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1004..1389
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     misc_RNA        complement(1630..1705)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(1726..2145)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        complement(2421..2496)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(2517..2936)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        complement(3572..3647)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(3668..4087)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     CDS             complement(join(4763..5476,5477..5479))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(4766..5476,5477..5479))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4808..4829)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5157..5176
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5416..5437
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(5496..6087)
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     enhancer        6091..6573
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     protein_bind    6588..6973
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     polyA_signal    7232..7353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7269..7288)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7323..7342
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7358..7777
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        7798..7873
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        8148..8567
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        8588..8663
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    8925..8994
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   9464..9498
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9505..9523)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9544..9560)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9544..9560)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9557..9579)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9568..9584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9592..9598,9599..9616,9617..9622))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9637..9658
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9775..9792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9946..10534)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10026..10045)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10705..11496,11497..11565))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11328..11347
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11566..11670)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaatta cgcgtatgca tacgcattaa gcgaacatta aaaagatgtg aaaacataac
     1441 tattatgtct aaataaacac acgtcagatg tatgtacgtc aacggaaaac cattgtctat
     1501 atattacaat tactaaatac ataccaaatt gaatacatat tgatgaaaaa taataaatac
     1561 tggcgaaagc aaaaaaacga aacattttta ttttattgaa caactctcag gctccaggta
     1621 ggcaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
     1681 aacttgctat ttctagctct aaaacccgat ctggttaccg cattgcgacg ttaaattgaa
     1741 aataggtcta tatatacgaa ctgagtctgg aaaaagaagt tgagaattat aaaaagtagt
     1801 gagcactggc gccctctctg cttggcgagc taaccttttc gcctcttggc tgagtaggtg
     1861 gcgtttcatt ctactctgta aaattaatgt agaattgaaa cactatggtc aaaaaatact
     1921 taggggcata agttataaaa cgtatgaaat gaatttttat caacctgggc tattcaaaaa
     1981 ttttcgaatt attttatgta ttttttttaa tcgtttttca ttataggtta aaatacactt
     2041 taaaaggaat tctttcctgt aaaataaata taaataaata tgctttattg acagaaaatt
     2101 tgatgttttt gtatttgagt aggagcaatc acaggtgagc aaaaagtgta tcactgacag
     2161 ctcgactctc tacgatatct aagctgatgc atacgcatta agcgaacatt aaaaagatgt
     2221 gaaaacataa ctattatgtc taaataaaca cacgtcagat gtatgtacgt caacggaaaa
     2281 ccattgtcta tatattacaa ttactaaata cataccaaat tgaatacata ttgatgaaaa
     2341 ataataaata ctggcgaaag caaaaaaacg aaacattttt attttattga acaactctca
     2401 ggctccaggt aggcaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
     2461 agccttattt taacttgcta tttctagctc taaaacttcc ggagccggta ccgccacgac
     2521 gttaaattga aaataggtct atatatacga actgagtctg gaaaaagaag ttgagaatta
     2581 taaaaagtag tgagcactgg cgccctctct gcttggcgag ctaacctttt cgcctcttgg
     2641 ctgagtaggt ggcgtttcat tctactctgt aaaattaatg tagaattgaa acactatggt
     2701 caaaaaatac ttaggggcat aagttataaa acgtatgaaa tgaattttta tcaacctggg
     2761 ctattcaaaa attttcgaat tattttatgt atttttttta atcgtttttc attataggtt
     2821 aaaatacact ttaaaaggaa ttctttcctg taaaataaat ataaataaat atgctttatt
     2881 gacagaaaat ttgatgtttt tgtatttgag taggagcaat cacaggtgag caaaaaggcg
     2941 cgcctcctga ccgtccatcg caataaaatg agccattatg agatcgaaag ggtctacgaa
     3001 aatccgttcg gaaaaaatca gaaaatcatc aaagccgaat ataattaaaa tgtattacta
     3061 gctaaagaaa tcatcactaa tatagaatgt agaatgaacc atgtatatag atactaatgt
     3121 atcgtaagac tttcaaaagt ctacaagaca ttaaatgaca agttgacttt aaatttcaaa
     3181 taaataattt attttttcta taagcaataa catttttgct aaattaagac ttggtaatta
     3241 ggtaatacta ttgttgttct atggaatatt cgatcgaaac attcttatca gtctcaaaaa
     3301 cttaaaacaa acttataata taacccatat gcctaggatg catacgcatt aagcgaacat
     3361 taaaaagatg tgaaaacata actattatgt ctaaataaac acacgtcaga tgtatgtacg
     3421 tcaacggaaa accattgtct atatattaca attactaaat acataccaaa ttgaatacat
     3481 attgatgaaa aataataaat actggcgaaa gcaaaaaaac gaaacatttt tattttattg
     3541 aacaactctc aggctccagg taggcaaaaa agcaccgact cggtgccact ttttcaagtt
     3601 gataacggac tagccttatt ttaacttgct atttctagct ctaaaacaca tctttagacc
     3661 ccgaggccga cgttaaattg aaaataggtc tatatatacg aactgagtct ggaaaaagaa
     3721 gttgagaatt ataaaaagta gtgagcactg gcgccctctc tgcttggcga gctaaccttt
     3781 tcgcctcttg gctgagtagg tggcgtttca ttctactctg taaaattaat gtagaattga
     3841 aacactatgg tcaaaaaata cttaggggca taagttataa aacgtatgaa atgaattttt
     3901 atcaacctgg gctattcaaa aattttcgaa ttattttatg tatttttttt aatcgttttt
     3961 cattataggt taaaatacac tttaaaagga attctttcct gtaaaataaa tataaataaa
     4021 tatgctttat tgacagaaaa tttgatgttt ttgtatttga gtaggagcaa tcacaggtga
     4081 gcaaaaacct aggtcgaatc gctatccaag ccagctccat tgtcggcatc gtgctcattc
     4141 tattgacgct ggcagatttg gttttggcgc tatgggaccc gttcggttac aacaacatgt
     4201 ttccgcgcga gtttcccgac gacatgtcgc gcacgttcct gactgcgtac tttgagagtt
     4261 tcgacaacac cacgtccaga gaaatcatag agtttatgcc cgagttcttt tcggaaatgg
     4321 tcgaaacgga cgatgacgcc acgtttgaat ctctatttca tttattagat tatgtggcat
     4381 ctttagaagt taattccgac ggccaaatgt taaacttgga ggagggtgat gaaattgagg
     4441 attttgacga atctactttg gtggggcaag cgttagccac tagctcgcta tacactcgca
     4501 tggagtttat gcagtacacg tttaggcaaa acacactatt gtctatgaac aaagaaaaca
     4561 acaattttaa tcaaataatc atgggtttat ttgcaacaaa cacaattgtg gcgtttacag
     4621 catttgttat acacacagaa ctcatatttt ttatattttt cgtaatcttc ctaatgatca
     4681 cattttatta cataatcaaa gaatcgtacg aatattataa aacaattgat ttgttatttt
     4741 aaaaacgatt catggatccc tattacttgt acagctcgtc catgccgaga gtgatcccgg
     4801 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     4861 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     4921 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     4981 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     5041 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     5101 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     5161 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     5221 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     5281 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     5341 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     5401 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     5461 gctcctcgcc cttgctcatt ttgttaatta agtcacttgg ttgttcacga tcttgtcgcc
     5521 gccagtgtca acttgcaact gaaacaatat ccaacatgaa cgtcaattta tactgcccta
     5581 atggcgaaca cgataacaat atttctttta ttatgccctc taaaaccaac gcggttatcg
     5641 tttatttatt caaattagat atagaacatc cgccgacata caatgttaat gcaaaaacgc
     5701 gtttggtgag cggatacgaa aacagtcggc cgataaacat taatctgagg tcgataacac
     5761 cgtccttgaa cggaacacga ggagcgtacg tgatcagctg cattcgcgcg ccgcgccttt
     5821 atcgagattt atttgcatac aacaagtaca ctgcgccgtt gggatttgtg gtaacgcgca
     5881 cacatgcaga gctgcaagtg tggcacattt tgtctgtgcg caaaaccttt gaagccaaaa
     5941 gtacgaggtc cgttacgggc atgctagcgc acacggacaa tggacccgac aaattctacg
     6001 ccaaggattt aatgataatg tcgggcaacg tatccgttca ttttatcaat aacctacaaa
     6061 aatgtcgcgc gcatcacaaa gacatcgacg cgcgtagaat tctacccgta aagcgagttt
     6121 agttatgagc catgtgcaaa acatgacatc agcttttatt tttataacaa atgacatcat
     6181 ttcttgattg tgttttacac gtagaattct actcgtaaag cgagttcagt tttgaaaaac
     6241 aaatgacatc atctttttga ttgtgcttta caagtagaat tctacccgta aatcaagttc
     6301 ggttttgaaa aacaaatgag tcatattgta tgatatcata ttgcaaaaca aatgactcat
     6361 caatcgatcg tgcgttacac gtagaattct actcgtaaag cgagtttatg agccgtgtgc
     6421 aaaacatgac atcatctcga tttgaaaaac aaatgacatc atccactgat cgtgcgttac
     6481 aagtagaatt ctactcgtaa agccagttcg gttatgagcc gtgtgcaaaa catgacatca
     6541 gcttatgact catacttgat tgtgttttac gcgactagta ggtacctcac gtaataagtg
     6601 tgcgttgaat ttattcgcaa aaacattgca tattttcggc aaagtaaaat tttgttgcat
     6661 accttatcaa aaaataagtg ctgcatactt tttagagaaa ccaaataatt ttttattgca
     6721 tacccgtttt taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacttt
     6781 gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat acccgttttt
     6841 aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatacgttg acgaaacaaa
     6901 ttttcgttgc atacccaata aaagattatt atattgcata ccttttcttg ccataccatt
     6961 tagccgatca attccgcggc atactcggtg gcctccccac caccaacttt tttgcactgc
     7021 aaaaaaacac gcttttgcac gcgggcccat acatagtaca aactctacgt ttcgtagact
     7081 attttacata aatagtctac accgttgtat acgctccaaa tacactacca cacattgaac
     7141 ctttttgcag tgcaaaaaag tacgtgtcgg cagtcacgta ggccggcctt atcgggtcgc
     7201 gtcctgtcac gtacgaatca cattatcggt taacttgttt attgcagctt ataatggtta
     7261 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7321 ttgtggtttg tccaaactca tcaatgtatc ttaaagcttt ttgctcacct gtgattgctc
     7381 ctactcaaat acaaaaacat caaattttct gtcaataaag catatttatt tatatttatt
     7441 ttacaggaaa gaattccttt taaagtgtat tttaacctat aatgaaaaac gattaaaaaa
     7501 aatacataaa ataattcgaa aatttttgaa tagcccaggt tgataaaaat tcatttcata
     7561 cgttttataa cttatgcccc taagtatttt ttgaccatag tgtttcaatt ctacattaat
     7621 tttacagagt agaatgaaac gccacctact cagccaagag gcgaaaaggt tagctcgcca
     7681 agcagagagg gcgccagtgc tcactacttt ttataattct caacttcttt ttccagactc
     7741 agttcgtata tatagaccta ttttcaattt aacgtcgggc ggcagcggcg ggaatgggtt
     7801 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     7861 accgagtcgg tgcttttttg cctacctgga gcctgagagt tgttcaataa aataaaaatg
     7921 tttcgttttt ttgctttcgc cagtatttat tatttttcat caatatgtat tcaatttggt
     7981 atgtatttag taattgtaat atatagacaa tggttttccg ttgacgtaca tacatctgac
     8041 gtgtgtttat ttagacataa tagttatgtt ttcacatctt tttaatgttc gcttaatgcg
     8101 tatgcatctc cgttgtatgt tgtagcgtct gtgaagcgtc ctactccttt ttgctcacct
     8161 gtgattgctc ctactcaaat acaaaaacat caaattttct gtcaataaag catatttatt
     8221 tatatttatt ttacaggaaa gaattccttt taaagtgtat tttaacctat aatgaaaaac
     8281 gattaaaaaa aatacataaa ataattcgaa aatttttgaa tagcccaggt tgataaaaat
     8341 tcatttcata cgttttataa cttatgcccc taagtatttt ttgaccatag tgtttcaatt
     8401 ctacattaat tttacagagt agaatgaaac gccacctact cagccaagag gcgaaaaggt
     8461 tagctcgcca agcagagagg gcgccagtgc tcactacttt ttataattct caacttcttt
     8521 ttccagactc agttcgtata tatagaccta ttttcaattt aacgtcggat tgtcaactac
     8581 ttgccccgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     8641 aaaaagtggc accgagtcgg tgcttttttg cctacctgga gcctgagagt tgttcaataa
     8701 aataaaaatg tttcgttttt ttgctttcgc cagtatttat tatttttcat caatatgtat
     8761 tcaatttggt atgtatttag taattgtaat atatagacaa tggttttccg ttgacgtaca
     8821 tacatctgac gtgtgtttat ttagacataa tagttatgtt ttcacatctt tttaatgttc
     8881 gcttaatgcg tatgcataag cttgtcgacg atgtaggtca cggtctcgaa gccgcggtgc
     8941 gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccacctcacc catctggtcc
     9001 atcatgatga acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg gcgcaccggg
     9061 aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg acgtgcgacg
     9121 gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt tctcgacggt cacggcgggc
     9181 atgtcgacag atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa
     9241 catgaaataa caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat
     9301 catgcgtcat tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt
     9361 gacaagcacg cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac
     9421 ggattcgcgc tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg
     9481 cagactatct ttctagggtt aatcccttta gtgagggtta atttcgagct tggcgtaatc
     9541 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9601 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9661 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9721 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9781 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     9841 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9901 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9961 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10021 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10081 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10141 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10201 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10261 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10321 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10381 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10441 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10501 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10561 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10621 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10681 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10741 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10801 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10861 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10921 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10981 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11041 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11101 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11161 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11221 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11281 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11341 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11401 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11461 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11521 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11581 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11641 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
