LOCUS       sequence-355017-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BioBrick pSB1C3 plasmid encoding a promoter-less version of 
            mRFP1_Orange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Bao L, Menon PNK, Liljeruhm J, Forster AC
  TITLE     Overcoming chromoprotein limitations by engineering a red 
            fluorescent protein.
  JOURNAL   Anal Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936.
  PUBMED    32891596
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936."
COMMENT     Sequence Label: promoter-less mRFP1_Orange
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             23..34
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             41..718
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFCYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    747..767
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 gaattcgcgg ccgcttctag agaaagagga gaaatactag atggcttcct ccgaagacgt
       61 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
      121 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
      181 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttctgcta
      241 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
      301 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
      361 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
      421 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
      481 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
      541 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
      601 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
      661 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata
      721 acgctgatag tgctagtgta gatcgctact agtagcggcc gctgcag
//
