LOCUS       sequence-355034-        3068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CC10
FEATURES             Location/Qualifiers
     source          1..3068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(235..253)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(258..274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      679..1267
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1168..1187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1394..1399,1400..1416,1417..1422)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1470..2660
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1822..1841)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(2835..2853)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2858..2874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
       61 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
      121 tcttgcggag aactgtgaat gcgcaaacca acccttgatc ggggaagaac agtatgtcga
      181 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat atcccctata
      241 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
      301 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accaaagctg
      361 gtctcgctct catgacggtg gcaaacaggc tcttgaaacc gtccaacgcc ttctaccagt
      421 tctctgtcaa gcccacggac taaccccagc gcaagttgta gcgattgcta gtcatgacgg
      481 tggcaaacag gctcttgaaa ccgtccaacg ccttctacca gttctctgtc aagcccacgg
      541 actaacccca gcgcaagttg tagcgattgc gtcatgagac catatctggt cgagccaccc
      601 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
      661 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      721 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      781 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      841 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      901 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      961 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1021 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1081 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1141 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1201 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1261 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1321 tcacatgttc tttcctgcgt tatccacaga atcaggggat aacgcaggaa agaacatgaa
     1381 ttaattctca tgtttgacag cttatcatcg attagcttta atgcggtagt ttatcacagt
     1441 taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc
     1501 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc
     1561 tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc
     1621 tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg
     1681 gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg
     1741 cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggccggc atcaccggcg
     1801 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     1861 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     1921 ggggactgtt gggcgccatc tccttacatg caccattcct tgcggcggcg gtgctcaacg
     1981 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgccgac
     2041 ccatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     2101 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     2161 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     2221 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     2281 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     2341 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     2401 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     2461 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg
     2521 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     2581 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     2641 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
     2701 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttgatc
     2761 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     2821 aaaataaaat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2881 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2941 cctcctctag accaaagctg gtctcgctct catgacggtg gcaaacaggc tcttgaaacc
     3001 gtccaacgcc ttctaccagt tctctgtcaa gcccacggac taaccccagc gcaagttgta
     3061 gcgattgc
//
