LOCUS       sequence-355037-        2498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pC9
FEATURES             Location/Qualifiers
     source          1..2498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(124..129,130..146,147..152))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(279..867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(359..378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1170..1186
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1170..1186
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1191..1210
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1191..1209
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1384..2498,1..76))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2203..2222
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
       61 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
      121 cgcattaaag ctaatcgatg ataagctgtc aaacatgaga attaattcat gttctttcct
      181 gcgttatccc ctgattctgt ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      901 gctcagtgga acgacgcgta actcacgtta agggattttg gtcatgggtg gctcgaccag
      961 atatggtctc aagaggcaat cgctacaact tgcgctgggg ttagtccgtg ggcttgacag
     1021 agaactggta gaaggcgttg gacggtttca agagcctgtt tgccaccgtc atgggaacga
     1081 gaccagcttt ggtctagagg aggcatgatg atatattttt atcttgtgca atgtaacatc
     1141 agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact
     1201 cactataggg gatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
     1261 agctcgacat actgttcttc cccgatcaag ggttggtttg cgcattcaca gttctccgca
     1321 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc
     1381 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
     1441 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
     1501 aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga
     1561 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     1621 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     1681 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
     1741 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     1801 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     1861 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     1921 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     1981 gttgaaggct ctcaagggca tgggtcggcg ctctccctta tgcgactcct gcattaggaa
     2041 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgtaa
     2101 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     2161 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     2221 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     2281 aatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     2341 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     2401 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     2461 ggaatggacg atatcccgca agaggcccgg cagtaccg
//
