LOCUS       sequence-355047-        5334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC8A3_STOP
FEATURES             Location/Qualifiers
     source          1..5334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2891..2990)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3007..3026)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3008..3026)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3031..3047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3031..3047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3149..3168)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3160..3969
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3227..3246)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4116..4704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4605..4624
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4858..4875
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5034..5061)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5153..5239)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5288..5310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5302..5319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5303..5319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc ctggctgaga
      121 ctgcagcccc tgacaagcgc ctttctgcac ttcggcctgg tgacctttgt gctgttcctg
      181 aacggactga gggcagaggc aggaggatcc ggcgacgtgc cttctaccgg ccagaacaat
      241 gagagctgtt ccggcagctc cgattgcaag gagggcgtga tcctgccaat ctggtacccc
      301 gagaatcctt ccctgggcga caagatcgcc cgcgtgatcg tgtactttgt ggccctgatc
      361 tatatgttcc tgggcgtgtc tatcatcgcc gatcgcttca tggccagcat cgaagtgatc
      421 acatcccagg agcgggaggt gaccatcaag aagccaaacg gcgagacatc caccacaacc
      481 atccgggtgt ggaacgagac agtgtctaat ctgaccctga tggcactggg atctagcgcc
      541 ccagagatcc tgctgtccct gatcgaggtg tgcggacacg gcttcatcgc aggcgacctg
      601 ggacctagca ccatcgtggg atccgccgcc tttaatatgt tcatcatcat cggcatctgc
      661 gtgtacgtga tccccgatgg cgagacaaga aagatcaagc acctgagggt gttctttatc
      721 accgccgcct ggtccatctt tgcctacatc tggctgtata tgatcctggc cgtgttctct
      781 cctggagtgg tgcaagtgtg ggagggactg ctgaccctgt tctttttccc cgtgtgcgtg
      841 ctgctggcat gggtggcaga caagcgcctg ctgttctaca agtatatgca caagaagtat
      901 cgcacagata agcaccgggg catcatcatc gagaccgagg gcgatcaccc aaagggcatc
      961 gagatggacg gcaagatgat gaacagccac tttctggatg gcaatctggt gcccctggag
     1021 ggcaaggagg tggacgagtc ccggagagag atgatcagaa tcctgaagga tctgaagcag
     1081 aagcaccccg agaaggacct ggatcagctg gtggagatgg ccaactacta tgccctgtct
     1141 caccagcaga agagcagagc cttctacagg atccaggcca caaggatgat gaccggcgcc
     1201 ggcaatatcc tgaagaagca cgcagcagag caggccaaga aggcatcctc tatgagcgag
     1261 gtgcacacag atgagcccga ggactttatc tccaaggtgt ttttcgaccc ttgctcttac
     1321 cagtgtctgg agaactgcgg agccgtgctg ctgaccgtgg tgcggaaggg cggcgacatg
     1381 tctaagacaa tgtacgtgga ttataagacc gaggacggaa gcgccaatgc aggagcagat
     1441 tatgagttca cagagggaac cgtggtgctg aagccaggag agacccagaa ggagttttct
     1501 gtgggcatca tcgacgatga catcttcgag gaggacgagc actttttcgt gagactgagc
     1561 aacgtgagga tcgaggagga gcagccagag gagggaatgc cacctgccat ctttaatagc
     1621 ctgcctctgc caagagccgt gctggcatcc ccttgcgtgg caacagtgac catcctggat
     1681 gacgatcacg ccggcatctt tacattcgag tgcgatacca tccacgtgtc tgagagcatc
     1741 ggcgtgatgg aggtgaaggt gctgcgcaca agcggagcaa ggggaaccgt gatcgtgcct
     1801 ttcaggacag tggagggaac cgcaaaggga ggaggagagg attttgagga cacctacggc
     1861 gagctggagt tcaagaacga cgagacagtg aagaccatcc gcgtgaagat cgtggatgag
     1921 gaggagtatg agcggcagga gaacttcttc atcgccctgg gcgagccaaa gtggatggag
     1981 agaggcatct ccgccctgct gctgtctccc gatgtgacag acagaaagct gaccatggag
     2041 gaggaggagg ccaagaggat cgccgagatg ggcaagccag tgctgggcga gcaccccaag
     2101 ctggaagtga tcatcgagga gtcctacgag ttcaagacaa ccgtggacaa gctgatcaag
     2161 aagacaaacc tggccctggt ggtgggaacc cacagctggc gcgatcagtt tatggaggca
     2221 atcacagtgt ccgccgcagg cgacgaggat gaggacgagt ctggcgagga gcggctgcct
     2281 agctgttttg actacgtgat gcactttctg acagtgttct ggaaggtgct gttcgcctgc
     2341 gtgccaccca ccgagtattg tcacggctgg gcctgctttg ccgtgtctat cctgatcatc
     2401 ggcatgctga ccgccatcat cggcgacctg gcctctcact tcggctgtac aatcggcctg
     2461 aaggatagcg tgaccgccgt ggtgtttgtg gccttcggca catccgtgcc agacaccttt
     2521 gcctctaagg cagccgccct gcaggacgtg tacgcagacg ccagcatcgg caatgtgaca
     2581 ggctccaacg ccgtgaacgt gttcctggga atcggactgg catggtccgt ggcagccatc
     2641 tattgggccc tgcagggcca ggagtttcac gtgtctgccg gcaccctggc ctttagcgtg
     2701 acactgttca ccatctttgc cttcgtgtgc atcagcgtgc tgctgtacag gcgccggcct
     2761 cacctgggag gagagctggg aggaccaagg ggctgcaagc tggccacaac ctggctgttc
     2821 gtgagcctgt ggctgctgta tatcctgttc gccaccctgg aggcctactg ctatatcaag
     2881 ggcttctaag acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     2941 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     3001 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     3061 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     3121 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     3181 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3241 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     3301 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3361 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3421 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     3481 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3541 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     3601 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3661 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     3721 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3781 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3841 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3901 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3961 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     4021 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     4081 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4141 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4201 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4261 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4321 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4381 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4441 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4501 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4561 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4621 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4681 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4741 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4801 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4861 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4921 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4981 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     5041 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     5101 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     5161 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     5221 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     5281 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
