LOCUS       sequence-355048-        4455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A19_STOP
FEATURES             Location/Qualifiers
     source          1..4455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2012..2111)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2128..2147)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2129..2147)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2152..2168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2152..2168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2270..2289)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2281..3090
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2348..2367)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3237..3825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3726..3745
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3979..3996
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4155..4182)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4274..4360)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4409..4431
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4423..4440
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4424..4440
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggt gaggctggtg
      121 ctgccaaacc caggactgga cgcaaggatc ccatccctgg ccgagctgga gaccatcgag
      181 caggaggagg ccagctccag gcccaagtgg gataacaagg cccagtacat gctgacatgc
      241 ctgggctttt gcgtgggact gggaaacgtg tggaggttcc cttacctgtg ccagagccac
      301 ggaggaggag cctttatgat cccattcctg atcctgctgg tgctggaggg catccccctg
      361 ctgtacctgg agtttgccat cggacagcgg ctgaggagag gatccctggg cgtgtggtct
      421 agcatccacc cagccctgaa gggactggga ctggcctcta tgctgaccag cttcatggtg
      481 ggcctgtact ataacacaat catcagctgg atcatgtggt acctgtttaa ttctttccag
      541 gagcctctgc catggagcga ctgccctctg aacgagaatc agaccggcta tgtggacgag
      601 tgtgcccggt cctctccagt ggattacttt tggtatagag agaccctgaa tatctccaca
      661 tctatcagcg attccggctc tatccagtgg tggatgctgc tgtgcctggc ctgtgcctgg
      721 tctgtgctgt atatgtgcac catccggggc atcgagacca caggcaaggc cgtgtacatc
      781 accagcacac tgccctatgt ggtgctgaca atcttcctga tcagaggcct gaccctgaag
      841 ggcgccacaa acggcatcgt gtttctgttc acccccaatg tgacagagct ggcacagcct
      901 gacacctggc tggatgcagg agcacaggtg ttctttagct tttccctggc cttcggcggc
      961 ctgatctcct ttagctccta caactctgtg cacaacaatt gcgagaagga ctccgtgatc
     1021 gtgtctatca tcaatggctt cacctccgtg tacgtggcca tcgtggtgta ttctgtgatc
     1081 ggctttaggg ccacacagcg gtacgacgat tgtttcagca ccaacatcct gacactgatc
     1141 aatggctttg acctgcccga gggcaacgtg acacaggaga atttcgtgga catgcagcag
     1201 aggtgcaacg caagcgatcc tgcagcatac gcacagctgg tgtttcagac ctgtgatatc
     1261 aatgccttcc tgtccgaggc agtggaggga acaggactgg cctttatcgt gttcaccgag
     1321 gccatcacaa agatgcccct gagccctctg tggtccgtgc tgttctttat catgctgttt
     1381 tgcctgggcc tgtctagcat gttcggcaac atggagggag tggtggtgcc tctgcaggac
     1441 ctgcgcgtga tcccacctaa gtggccaaag gaggtgctga ccggcctgat ctgtctgggc
     1501 acattcctga tcggctttat cttcaccctg aattccggcc agtactggct gtctctgctg
     1561 gatagctatg ccggctccat ccctctgctg atcatcgcct tttgcgagat gttctctgtg
     1621 gtgtacgtgt atggcgtgga ccggtttaac aaggatatcg agttcatgat cggccacaag
     1681 cccaatatct tttggcaggt gacctggaga gtggtgtccc ctctgctgat gctgatcatc
     1741 ttcctgttct ttttcgtggt ggaggtgagc caggagctga catactccat ctgggatcct
     1801 ggctatgagg agttcccaaa gtctcagaag atcagctacc caaactgggt gtatgtggtg
     1861 gtggtcatcg tggcaggagt gccaagcctg accatcccag gctacgccat ctataagctg
     1921 atcaggaacc actgtcagaa gccaggcgac caccagggac tggtgtctac cctgagcaca
     1981 gcctccatga atggcgatct gaagtactaa gacccagctt tcttgtacaa agttggcatt
     2041 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2101 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2161 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2221 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2281 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     2341 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     2401 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     2461 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     2521 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     2581 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     2641 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     2701 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     2761 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     2821 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     2881 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     2941 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     3001 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     3061 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     3121 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     3181 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3241 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3301 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3361 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3421 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3481 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3541 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3601 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3661 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3721 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3781 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3841 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3901 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3961 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4021 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4081 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     4141 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     4201 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4261 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4321 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4381 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4441 cttaagctcg ggccc
//
