LOCUS       sequence-355050-        5463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A8_STOP
FEATURES             Location/Qualifiers
     source          1..5463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3020..3119)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3136..3155)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3137..3155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3160..3176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3160..3176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3278..3297)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3289..4098
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3356..3375)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4245..4833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4734..4753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4987..5004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5163..5190)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5282..5368)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5417..5439
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5431..5448
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5432..5448
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc acagctggag
      121 cggtctgcca tcagcggctt tagctccaag tcccggagaa actctttcgc ctacgatgtg
      181 aagagggagg tgtataatga ggagaccttc cagcaggagc acaagcgcaa ggcctctagc
      241 tccggcaaca tgaatatcaa catcaccaca tttcgccacc acgtgcagtg ccggtgtagc
      301 tggcacagat tcctgagatg cgtgctgaca atcttcccat ttctggagtg gatgtgcatg
      361 tacaggctga aggattggct gctgggcgac ctgctggcag gaatcagcgt gggactggtg
      421 caggtgccac agggactgac cctgtccctg ctggcaaggc agctgatccc acctctgaac
      481 atcgcctacg ccgccttttg ctctagcgtg atctatgtga tcttcggcag ctgtcaccag
      541 atgtccatcg gctctttctt tctggtgagc gccctgctga tcaatgtgct gaaggtgtcc
      601 ccttttaaca atggccagct ggtcatgggc tccttcgtga agaacgagtt tagcgcccca
      661 tcctacctga tgggctataa taagagcctg tccgtggtgg ccaccacaac ctttctgaca
      721 ggcatcatcc agctgatcat gggcgtgctg ggactgggct tcatcgcaac ctacctgccc
      781 gagtctgcca tgagcgccta tctggcagca gtggccctgc acatcatgct gagccagctg
      841 acattcatct ttggcatcat gatctctttc cacgccggcc ctatcagctt cttttacgac
      901 atcatcaatt attgcgtggc cctgccaaag gccaactcta ccagcatcct ggtgtttctg
      961 acagtggtgg tggccctgag aatcaataag tgtatcagga tctccttcaa ccagtacccc
     1021 atcgagttcc ctatggagct gtttctgatc atcggcttca ccgtgatcgc caacaagatc
     1081 agcatggcca cagagacctc ccagaccctg atcgatatga tcccctattc ttttctgctg
     1141 ccagtgacac ccgacttcag cctgctgcct aagatcatcc tgcaggcctt ctccctgtct
     1201 ctggtgtcct ctttcctgct gatctttctg ggcaagaaga tcgccagcct gcacaactac
     1261 tccgtgaatt ctaaccagga tctgatcgcc atcggcctgt gcaatgtggt gagctccttc
     1321 tttagatcct gcgtgttcac cggcgccatc gccaggacaa tcatccagga caagagcgga
     1381 ggccgccagc agttcgcatc cctggtgggc gccggcgtga tgctgctgct gatggtgaag
     1441 atgggccact tcttttatac actgcctaat gccgtgctgg ccggcatcat cctgagcaac
     1501 gtgatcccat acctggagac catcagcaat ctgccctccc tgtggaggca ggatcagtat
     1561 gactgcgccc tgtggatgat gaccttttct agctccatct tcctgggcct ggatatcggc
     1621 ctgatcatca gcgtggtgtc cgccttcttt atcacaaccg tgcgcagcca cagggcaaag
     1681 atcctgctgc tgggacagat cccaaatacc aacatctacc gctccatcaa cgactatcgg
     1741 gagatcatca caatccccgg cgtgaagatc tttcagtgct gttctagcat caccttcgtg
     1801 aacgtgtact atctgaagca caagctgctg aaggaggtgg atatggtgaa ggtgcctctg
     1861 aaggaggagg agatcttcag cctgtttaat tcctctgaca caaacctgca gggcggcaag
     1921 atctgcaggt gtttttgcaa ctgtgacgat ctggagccac tgcccagaat cctgtacacc
     1981 gagaggttcg agaacaagct ggatccagag gccagctcca tcaatctgat ccactgctct
     2041 cactttgaga gcatgaatac ctcccagaca gcctctgagg accaggtgcc ctacacagtg
     2101 tctagcgtga gccagaagaa ccagggccag cagtatgagg aagtggagga agtgtggctg
     2161 cctaacaatt cctctcgcaa tagctcccct ggcctgccag atgtggcaga gtctcagggc
     2221 aggcgcagcc tgatccctta ctctgacgcc agcctgctgc catccgtgca caccatcatc
     2281 ctggatttct ccatggtgca ctatgtggac tctaggggac tggtggtgct gaggcagatc
     2341 tgcaacgcct ttcagaatgc caacatcctg atcctgatcg ccggctgtca ctctagcatc
     2401 gtgagagcct tcgagaggaa tgatttcttt gacgccggca tcacaaagac ccagctgttt
     2461 ctgtccgtgc acgatgccgt gctgttcgcc ctgtctcgga aagtgatcgg ctcctctgag
     2521 ctgagcatcg acgagtccga gaccgtgatc cgggagacct actctgagac agataagaat
     2581 gacaacagca gatataagat gagctcctct tttctgggct cccagaagaa cgtgtctccc
     2641 ggcttcatca agatccagca gcctgtggag gaggagtccg agctggacct ggagctggag
     2701 tctgagcagg aggcaggact gggactggac ctggatctgg acagagagct ggagccagag
     2761 atggagccca aggccgagac agagaccaag acacagaccg agatggagcc tcagcctgag
     2821 acagagcccg agatggagcc caaccctaag agcaggccaa gggcacacac cttccctcag
     2881 cagcggtact ggcctatgta tcacccatcc atggcctcta cccagtccca gacacagacc
     2941 agaacatggt ctgtggagcg gagaaggcac ccaatggata gctactcccc cgagggcaat
     3001 tccaacgagg acgtgtaaga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     3061 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     3121 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     3181 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     3241 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     3301 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     3361 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     3421 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     3481 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     3541 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     3601 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     3661 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     3721 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     3781 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     3841 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     3901 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3961 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     4021 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     4081 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     4141 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     4201 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4261 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4321 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4381 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4441 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4501 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4561 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4621 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4681 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4741 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4801 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4861 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4921 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4981 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5041 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5101 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     5161 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     5221 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     5281 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     5341 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     5401 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     5461 ccc
//
