LOCUS       sequence-355051-        4785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A5_STOP
FEATURES             Location/Qualifiers
     source          1..4785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2342..2441)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2458..2477)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2459..2477)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2482..2498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2482..2498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2600..2619)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2611..3420
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2678..2697)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3567..4155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4056..4075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4309..4326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4485..4512)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4604..4690)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4739..4761
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4753..4770
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4754..4770
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ccacgccgag
      121 gagaatgaga tcctggccgc cacccagagg tactatgtgg agcgccccat cttcagccac
      181 cctgtgctgc aggagaggct gcacacaaag gacaaggtgc ccgattccat cgccgacaag
      241 ctgaagcagg cctttacctg cacacctaag aagatccgca acatcatcta tatgttcctg
      301 ccaatcacca agtggctgcc cgcctacaag tttaaggagt atgtgctggg cgatctggtg
      361 tctggcatca gcacaggcgt gctgcagctg cctcagggac tggccttcgc catgctggca
      421 gcagtgccac ctatcttcgg actgtacagc tccttttatc cagtgatcat gtactgtttc
      481 ctgggcacca gcaggcacat ctccatcgga ccctttgccg tgatcagcct gatgatcgga
      541 ggagtggccg tgagactggt gcctgacgat atcgtgatcc caggcggcgt gaacgcaacc
      601 aatggaacag aggcaaggga cgcactgaga gtgaaggtgg ccatgtctgt gaccctgctg
      661 agcggcatca tccagttctg cctgggcgtg tgccggttcg gctttgtggc catctacctg
      721 acagagcccc tggtgagagg ctttaccaca gccgccgccg tgcacgtgtt cacctccatg
      781 ctgaagtatc tgtttggcgt gaagacaaag aggtactctg gcatcttcag cgtggtgtat
      841 tccaccgtgg ccgtgctgca gaatgtgaag aacctgaacg tgtgctctct gggcgtgggc
      901 ctgatggtgt ttggcctgct gctgggcggc aaggagttca acgagcggtt caaggagaag
      961 ctgcctgccc caatccccct ggagttcttt gcagtggtca tgggaaccgg aatcagcgcc
     1021 ggcttcaatc tgaaggagtc ctacaacgtg gacgtggtgg gcaccctgcc actgggactg
     1081 ctgccacccg ccaatcctga cacaagcctg ttccacctgg tgtatgtgga tgccatcgcc
     1141 atcgccatcg tgggcttttc tgtgaccatc agcatggcca agacactggc caataagcac
     1201 ggctaccagg tggatggcaa ccaggagctg atcgccctgg gcctgtgcaa ctccatcggc
     1261 tctctgttcc agaccttttc catctcttgt agcctgtcca gatctctggt gcaggaggga
     1321 accggaggca agacacagct ggcaggatgt ctggcctccc tgatgatcct gctggtcatc
     1381 ctggccacag gcttcctgtt tgagtccctg ccacaggccg tgctgtctgc catcgtgatc
     1441 gtgaatctga agggcatgtt catgcagttt tccgacctgc ctttcttttg gcggacctct
     1501 aagatcgagc tgacaatctg gctgaccaca ttcgtgtcta gcctgtttct gggcctggat
     1561 tacggcctga tcaccgccgt gatcatcgcc ctgctgaccg tgatctacag aacacagagc
     1621 ccatcctata aggtgctggg caagctgccc gagacagacg tgtacatcga catcgatgcc
     1681 tatgaggagg tgaaggagat ccctggcatc aagatcttcc agatcaatgc cccaatctac
     1741 tatgccaatt ccgatctgta ctctaacgcc ctgaagagga agaccggagt gaacccagcc
     1801 gtgatcatgg gagcaaggag aaaggccatg cgcaagtatg ccaaggaagt gggcaacgcc
     1861 aatatggcaa acgcaaccgt ggtgaaggca gacgcagagg tggacggaga ggatgccaca
     1921 aagcctgagg aggaggatgg cgaggtgaag tatcctccaa tcgtgatcaa gtccaccttc
     1981 ccagaggaga tgcagcggtt tatgccccct ggcgataatg tgcacaccgt gatcctggac
     2041 ttcacacagg tgaactttat cgattctgtg ggcgtgaaga ccctggccgg catcgtgaag
     2101 gagtacggcg acgtgggcat ctacgtgtat ctggcaggat gcagcgccca ggtggtgaac
     2161 gatctgacaa ggaatcgctt ctttgagaac ccagccctgt gggagctgct gtttcactct
     2221 atccacgacg ccgtgctggg cagccagctg cgggaggccc tggcagagca ggaggcaagc
     2281 gccccaccat cccaggagga tctggagccc aacgccaccc ctgccacacc agaggcctaa
     2341 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2401 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2461 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2521 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     2581 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     2641 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2701 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     2761 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2821 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2881 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     2941 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     3001 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     3061 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3121 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     3181 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     3241 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     3301 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3361 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     3421 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     3481 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3541 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3601 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3661 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3721 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3781 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3841 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3901 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3961 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4021 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4081 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4141 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4201 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4261 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4321 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4381 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4441 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4501 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4561 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4621 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4681 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4741 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
