LOCUS       sequence-355062-        1922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Beltran J, Steiner PJ, Bedewitz M, Wei S, Peterson FC, Li Z, Hughes 
            BE, Hartley Z, Robertson NR, Medina-Cucurella AV, Baumer ZT, Leonard
            AC, Park SY, Volkman BF, Nusinow DA, Zhong W, Wheeldon I, Cutler SR,
            Whitehead TA
  TITLE     Rapid biosensor development using plant hormone receptors as 
            reprogrammable scaffolds.
  JOURNAL   Nat Biotechnol. 2022 Jun 20. pii: 10.1038/s41587-022-01364-5. doi: 
            10.1038/s41587-022-01364-5.
  PUBMED    35726092
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jun 20. pii: 10.1038/s41587-022-01364-5. doi: 
            10.1038/s41587-022-01364-5."
COMMENT     Sequence Label: pJS647
FEATURES             Location/Qualifiers
     source          1..1922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(195..1010)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(195..1010)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     918..937
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1002..1021
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1235..1823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1724..1743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgagaaagag aatcggatct ggacggtggt tttggaatct tacgtcgttg atatgccgga
       61 aggtaactcg gaggatgata ctcgtatgtt tgctgatacg gttgtgaagc ttaatttgca
      121 gaaactcgcg acggttgctg aagctatggc tcgtatgaga cctcagcgtg ttacaaccaa
      181 ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
      241 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
      301 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
      361 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
      421 ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact
      481 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
      541 ttcattcgtg attgcgcctg agcgaggcga aatacgcgat cgctgttaaa aggacaatta
      601 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
      661 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
      721 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
      781 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
      841 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
      901 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
      961 gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     1021 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     1081 gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt
     1141 gctgagttga aggatcagtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1921 gt
//
