LOCUS       sequence-355065-       13013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one ""tet-on"" 3G lentiviral vector plasmid encoding human 
            cytomegalovirus UL148 ER resident glycoprotein with a C-terminal HA 
            tag fused to its cytoplasmic tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Siddiquey MNA, Zhang H, Nguyen CC, Domma AJ, Kamil JP
  TITLE     The Human Cytomegalovirus Endoplasmic Reticulum-Resident 
            Glycoprotein UL148 Activates the Unfolded Protein Response.
  JOURNAL   J Virol. 2018 Sep 26;92(20). pii: JVI.00896-18. doi: 
            10.1128/JVI.00896-18. Print 2018 Oct 15.
  PUBMED    30045994
REFERENCE   2  (bases 1 to 13013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2018-09-26"; volume: "92(20). pii: JVI.00896-18. doi"; pages:
            " 10.1128/JVI.00896-18. Print 2018 Oct 15"
COMMENT     Sequence Label: pOUPc-UL148-HA
FEATURES             Location/Qualifiers
     source          1..13013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    111..245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             413..1046
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1093..1218
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1715..1948
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2133..2177
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2475..2592
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2657..3031)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      3028..3037
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3041..3059)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3051..3098)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3147..3525
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3155..3173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3191..3209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3227..3245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3263..3281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3299..3317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3335..3353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3371..3389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4485..4511
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4529..5740
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5577..5596
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5747..5780
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5787..6404,6405..6410,6411..6446,6447..6449,6450..6485,
                     6486..6488,6489..6524,6525..6533)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6582..7154
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6749..6766)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6976..6995
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7157..7759
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7656..7676
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7781..8369
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7834..7854)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8252..8263)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8576..8809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8910..8927)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8916..9140
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9186..9614
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9273..9292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9483..9504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9623..9643)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9628..9957
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9808..9943
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9870..9889
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10780..10901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10950..10966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10950..10966)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10963..10985)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10974..10990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10998..11004,11005..11022,11023..11028))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11043..11064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11181..11198)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11352..11940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11432..11451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12111..12902,12903..12971))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12734..12753
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(12972..13013,1..63))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
       61 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta
      121 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      181 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
      241 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
      301 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
      361 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
      421 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
      481 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
      541 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
      601 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
      661 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
      721 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
      781 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
      841 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      901 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      961 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     1021 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     1081 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     1141 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     1201 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1261 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1321 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1381 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1441 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1501 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1561 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1621 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1681 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1741 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1801 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1861 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1921 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1981 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     2041 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     2101 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     2161 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     2221 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     2281 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2341 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2401 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     2461 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     2521 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     2581 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc
     2641 ccgctctagt ccggaatcag tcctgctcct cggccacgaa gtgcacgcag ttgccggccg
     2701 ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc atggccggcc
     2761 cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac agctcgtcca
     2821 ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc tggaccgcgc
     2881 tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc acgaagtccc
     2941 gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga
     3001 gcaccggaac ggcactggtc aacttggcca tggtggccct cctatagtga gtcgtattat
     3061 actatgccga tatactatgc cgatgattaa ttgtcaacac gtgctgcagg tccgaggttc
     3121 taggctagcg agactagcga cgagttggct ttactcccta tcagtgatag agaacgtatg
     3181 aagagtttac tccctatcag tgatagagaa cgtatgcaga ctttactccc tatcagtgat
     3241 agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac cagtttactc
     3301 cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag agaacgtata
     3361 tccagtttac tccctatcag tgatagagaa cgtataagct ttaggcgtgt acggtgggcg
     3421 cctataaaag cagagctcag gtagtgaacc gtcagatcgc ctggagcaat tccacaacac
     3481 ttttgtctta taccaacttt ccgtaccact tcctaccctc gtaaaccggt gccaccatgt
     3541 tgcgcttgct gttcacgctc gtcctgctgg ccctccacgg gccgtctgtc aatgctagcc
     3601 gcgactatgt gcatgttcgg ctattgagct accgaggcga ccccctggtc ttcaagcaca
     3661 ctttttcggg tgtgcgtcga cccttcaccg agctaggctg ggctgcgtgt cgcgactggg
     3721 acagtatgca ttgcacgccc ttctggtcta ccgatccgga gcagatgacc gactcggtgc
     3781 ggcgttacag cacagtgagc cccggcaagg aagtgacgct tcagcttcac gggaaccaaa
     3841 ccgtacagcc gtcgtttcta agctttacgt gccgcctgca gctagaaccc gtggtggaaa
     3901 atgttggcct ctacgtggcc tacgtggtca acgacggtga acgcccacag cagtttttta
     3961 caccgcaggt agacgtggta cgctttgctc tatatctaga gacgctctcc cggatcgtgg
     4021 aaccgttaga atcaggtcgc ctggcagtgg aatttgatac gcctgaccta gctctggcgc
     4081 ccgatttagt aagcagcctc ttcgtggccg gacacggcga gaccgacttt tacatgaact
     4141 ggacgctgcg tcgcagtcag acccactacc tggaggagat ggccttacag gtggagattc
     4201 taaagccccg cggcgtacgt caccgcgcta ttatccacca tccgaagcta cagccgggcg
     4261 ttggcctgtg gatagatttc tgcgtgtacc gctacaacgc gcgcctgacc cgcggctacg
     4321 tacgatacac cctgtcaccg aaagcgcgct tgcccgcaaa agcagagggt tggctggtgt
     4381 cactagacag attcatcgtg cagtacctca acacattgct gattacaatg atggcggcga
     4441 tatgggctcg cgttttgata acctacctgg tgtcgcggcg tcggtaccca tacgacgtcc
     4501 cagactacgc ctagacgcgt taagatctgg cctccgcgcc gggttttggc gcctcccgcg
     4561 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     4621 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     4681 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     4741 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     4801 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     4861 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     4921 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg
     4981 gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt
     5041 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa
     5101 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg
     5161 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag
     5221 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg
     5281 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac
     5341 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc
     5401 agggtggggc cacctgccgg taggtgtgtg gtaggctttt ctccgtcgca ggacgcaggg
     5461 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg
     5521 ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa
     5581 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc
     5641 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt
     5701 gtccgctaaa ttctggccgt ttttggcttt tttgttagac ggatccataa cttcgtataa
     5761 tgtatgctat acgaagttat gccaccatgt ctagactgga caagagcaaa gtcataaacg
     5821 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     5881 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     5941 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     6001 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     6061 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     6121 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     6181 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     6241 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     6301 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     6361 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     6421 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     6481 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taaataactt
     6541 cgtatagcat acattatacg aagtatggcg cgccaattcc gcccctctcc ctcccccccc
     6601 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     6661 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     6721 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     6781 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     6841 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     6901 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     6961 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     7021 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     7081 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     7141 aaaacacgat aataccatgg ccaccgagta caagcccacg gtgcgcctcg ccacccgcga
     7201 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     7261 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     7321 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     7381 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggctcgcg
     7441 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     7501 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     7561 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     7621 tggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7681 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     7741 ccgcaagccc ggtgcctgat taattaagcg cgtctggaac aatcaacctc tggattacaa
     7801 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7861 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7921 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7981 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     8041 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     8101 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     8161 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     8221 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     8281 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     8341 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     8401 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     8461 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     8521 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
     8581 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     8641 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8701 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8761 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     8821 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8881 ccttgacatt gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8941 tgttgtttgc cccttccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     9001 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     9061 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     9121 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     9181 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9241 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9301 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     9361 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9421 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9481 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9541 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9601 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     9661 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     9721 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9781 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9841 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     9901 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9961 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgatgaa aaagcctgaa
    10021 ctcaccgcga cgtgaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
    10081 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
    10141 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
    10201 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
    10261 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
    10321 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
    10381 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
    10441 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
    10501 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
    10561 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
    10621 cccagcactc gtccgagggc aaaggaatag cacgtgctac gagatttcga ttccaccgcc
    10681 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    10741 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
    10801 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10861 cattctagtt gtggtttgtc caaactaatc aatgtatctt atcatgtctg tataccgtcg
    10921 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    10981 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    11041 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    11101 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    11161 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    11221 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    11281 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    11341 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11401 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    11461 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11521 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11581 gtcgttcgct ccaagctggg ctgtgtgcac taaccccccg ttcagcccga ccgctgcgcc
    11641 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    11701 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    11761 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    11821 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    11881 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    11941 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12001 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12061 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12121 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12181 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12241 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12301 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12361 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12421 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12481 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12541 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12601 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12661 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    12721 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    12781 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    12841 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    12901 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    12961 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg tta
//
