LOCUS       sequence-355067-       13103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-on ""tet-on"" 3G lentiviral vector plasmid encoding rhesus 
            cytomegalovirus Rh159 (NK cell evasion protein) with a C-terminal HA
            tag fused to its cytoplasmic tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Siddiquey MNA, Zhang H, Nguyen CC, Domma AJ, Kamil JP
  TITLE     The Human Cytomegalovirus Endoplasmic Reticulum-Resident 
            Glycoprotein UL148 Activates the Unfolded Protein Response.
  JOURNAL   J Virol. 2018 Sep 26;92(20). pii: JVI.00896-18. doi: 
            10.1128/JVI.00896-18. Print 2018 Oct 15.
  PUBMED    30045994
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2018-09-26"; volume: "92(20). pii: JVI.00896-18. doi"; pages:
            " 10.1128/JVI.00896-18. Print 2018 Oct 15"
COMMENT     Sequence Label: pOUPc-Rh159-HA
FEATURES             Location/Qualifiers
     source          1..13103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(5..23)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(15..62)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        111..489
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    119..137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    263..281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    299..317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    335..353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1478..1504
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1524..2735
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2572..2591
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    2742..2775
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(2782..3399,3400..3405,3406..3441,3442..3444,3445..3480,
                     3481..3483,3484..3519,3520..3528)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3577..4149
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3744..3761)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3971..3990
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4152..4754
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4651..4671
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4776..5364
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4829..4849)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5247..5258)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5571..5804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5905..5922)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5911..6135
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6181..6609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6268..6287)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6478..6499
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6618..6638)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6623..6952
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6803..6938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6865..6884
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    7775..7896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7945..7961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7945..7961)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7958..7980)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7969..7985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7993..7999,8000..8017,8018..8023))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8038..8059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8176..8193)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8347..8935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8427..8446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9106..9897,9898..9966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9729..9748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9967..10071)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10119..10253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             10421..11054
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11101..11226
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11782..12015
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12200..12244
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12542..12659
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(12724..13098)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      join(13095..13103,1)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccctcctata gtgagtcgta ttatactatg ccgatatact atgccgatga ttaattgtca
       61 acacgtgctg caggtccgag gttctaggct agcgagacta gcgacgagtt ggctttactc
      121 cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag agaacgtatg
      181 cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc tatcagtgat
      241 agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta cagtttactc
      301 cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag agaacgtata
      361 agctttaggc gtgtacggtg ggcgcctata aaagcagagc tcaggtagtg aaccgtcaga
      421 tcgcctggag caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
      481 cctcgtaaac ggatccacca tggcctacaa cagcttcctt ctcagctgcc ttaccatcgc
      541 acttttactg cacggtcttc caacatcaac tagcgctaaa gacatggcag gctacgtaca
      601 cgttcatatg cttaacgcac ctgaagacgc cgcttctacg tctgcgccag ttatccaact
      661 agccaacggc tttattacct gcagaaaatt tacagagctc ggttgggcac tgtgtactag
      721 ctgggatgat atggagtgtt ttccgttttg gcgaaggact ttctcggaaa ctaaaaatat
      781 cactgtgctt tcagacgatc catactatgc cggcgttgtg ttgacagtac ggacgcttct
      841 tggtgactcc ctagagctta catcttatct cggggtcagg ccttcgattg ccaatgtggg
      901 ctactatgta atgtacctgt catatgctaa ggcgctcccg gcaaaattta cccacaacga
      961 tgtggactat gtgaggtttg ccatatacct tcacaccgaa attgatgcgc agcaaactcc
     1021 tgacaacaac ttcgtcttta cgttcgccac acccgggaca tttgaaaccc cagacctcat
     1081 gagtagtttt gtgattggaa ccgatgatgg acaaaatagc accttgaatt tcaccactgt
     1141 tggcggagaa aaacaccgct acaacaacgt ggagctcagc tcagaaatga agggcaattg
     1201 tgtgtatcac tcggctatta tccggaacgt cgccagctcg caatcttggg tgtcgtttga
     1261 cttctcccta tattggcgac gtaccaaaat tgcatatagt tacgtgcagt attatttccc
     1321 gaaaccactc cattcacctg aacccatagg tcctgcctgg cagagaatct accattactt
     1381 tagcctcctg acgcagcttc gctacggaac agtagcgatc accgtggtgc tcattttcct
     1441 tataggtgca cttctaaaac gcagtcgtga agctcattac ccatacgacg tcccagacta
     1501 cgcctagtga attcttaaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1561 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1621 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1681 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     1741 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     1801 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     1861 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     1921 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     1981 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2041 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2101 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2161 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2221 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2281 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2341 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2401 ggggccacct gccggtaggt gtgtggtagg cttttctccg tcgcaggacg cagggttcgg
     2461 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2521 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2581 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2641 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     2701 ctaaattctg gccgtttttg gcttttttgt tagacggatc cataacttcg tataatgtat
     2761 gctatacgaa gttatgccac catgtctaga ctggacaaga gcaaagtcat aaacggagct
     2821 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     2881 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     2941 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     3001 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     3061 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     3121 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     3181 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     3241 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     3301 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     3361 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     3421 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg
     3481 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaaat aacttcgtat
     3541 agcatacatt atacgaagta tggcgcgcca attccgcccc tctccctccc ccccccctaa
     3601 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     3661 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     3721 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     3781 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     3841 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     3901 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     3961 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     4021 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     4081 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     4141 acgataatac catggccacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4201 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4261 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4321 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4381 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc tcgcgcatgg
     4441 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     4501 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     4561 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgctgggg
     4621 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     4681 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     4741 agcccggtgc ctgattaatt aagcgcgtct ggaacaatca acctctggat tacaaaattt
     4801 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4861 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4921 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4981 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5041 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5101 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5161 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     5221 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5281 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5341 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     5401 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     5461 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     5521 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     5581 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     5821 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
     5881 acattgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5941 tttgcccctt ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6001 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6061 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6121 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6601 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6781 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6901 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6961 gggagcttgt atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac
     7021 cgcgacgtga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     7081 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     7141 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     7201 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     7261 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     7321 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     7381 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     7441 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     7501 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     7561 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     7621 cactcgtccg agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt
     7681 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7741 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7801 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7861 tagttgtggt ttgtccaaac taatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7921 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7981 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8101 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8581 tcgctccaag ctgggctgtg tgcactaacc ccccgttcag cccgaccgct gcgccttatc
     8641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8761 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9421 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9721 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10081 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
    10141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10201 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    10261 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    10321 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10381 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
    10441 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    10501 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    10561 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    10621 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    10681 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    10741 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    10801 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    10861 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    10921 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    10981 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    11041 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    11101 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    11161 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    11221 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg araaaawkmk gkkwrggmsa
    11281 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    11341 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    11401 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    11461 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    11521 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    11581 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
    11641 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
    11701 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
    11761 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
    11821 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
    11881 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
    11941 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
    12001 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
    12061 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
    12121 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
    12181 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
    12241 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
    12301 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
    12361 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
    12421 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
    12481 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
    12541 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    12601 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
    12661 gggtttatta cagggacagc agagatccag tttggttagt accgggcccg ctctagtccg
    12721 gaatcagtcc tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc
    12781 gaactcccgc ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg
    12841 gaagttcgtg gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca
    12901 cacccaggcc agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt
    12961 cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag
    13021 ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc
    13081 actggtcaac ttggccatgg tgg
//
