LOCUS       sequence-355068-       13728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All in one ""tet-on"" lentivirus vector that expresses human 
            cytomegalovirus UL148 fused to an mCherry tag at its cytoplasmic 
            tail. UL148 reorganizes the ER and activates the UPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13728)
  AUTHORS   Zhang H, Read C, Nguyen CC, Siddiquey MNA, Shang C, Hall CM, von 
            Einem J, Kamil JP
  TITLE     The Human Cytomegalovirus Nonstructural Glycoprotein UL148 
            Reorganizes the Endoplasmic Reticulum.
  JOURNAL   mBio. 2019 Dec 10;10(6). pii: mBio.02110-19. doi: 
            10.1128/mBio.02110-19.
  PUBMED    31822584
REFERENCE   2  (bases 1 to 13728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2019-12-10"; volume: "10(6). pii: mBio.02110-19. doi"; pages:
            " 10.1128/mBio.02110-19"
COMMENT     Sequence Label: pOUPc-UL148-mCherry
FEATURES             Location/Qualifiers
     source          1..13728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(75..179)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    227..361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             529..1162
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1209..1334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1831..2064
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2249..2293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2591..2708
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2773..3147)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      3144..3153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3157..3175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3167..3214)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3263..3641
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    3271..3289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3307..3325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3343..3361
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3379..3397
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3415..3433
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3451..3469
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3487..3505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4615..5325
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4760..4778)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5028..5047
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        5360..6571
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6408..6427
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    6578..6611
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6618..7235,7236..7241,7242..7277,7278..7280,7281..7316,
                     7317..7319,7320..7355,7356..7364)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7413..7985
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7580..7597)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7807..7826
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7988..8590
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8487..8507
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8612..9200
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8665..8685)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9083..9094)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9407..9640
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9741..9758)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9747..9971
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10017..10445
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10104..10123)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10314..10335
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10454..10474)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10459..10788
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10639..10774
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10701..10720
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11611..11732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11781..11797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11781..11797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11794..11816)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11805..11821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11829..11835,11836..11853,11854..11859))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11874..11895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12012..12029)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12183..12771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12263..12282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12942..13728,1..5,6..74))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13565..13584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
       61 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
      121 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
      181 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
      241 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      301 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
      361 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
      421 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
      481 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
      541 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
      601 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
      661 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
      721 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
      781 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
      841 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
      901 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
      961 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     1021 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     1081 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     1141 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     1201 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     1261 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     1321 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     1381 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     1441 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     1501 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1561 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1621 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     1681 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1741 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1801 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1861 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1921 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1981 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     2041 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     2101 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     2161 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     2221 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     2281 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     2341 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     2401 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2461 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     2521 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     2581 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     2641 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     2701 aaaattttcg ggtttattac agggacagca gagatccagt ttggttagta ccgggcccgc
     2761 tctagtccgg aatcagtcct gctcctcggc cacgaagtgc acgcagttgc cggccgggtc
     2821 gcgcagggcg aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga
     2881 ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc
     2941 gcgcacccac acccaggcca gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat
     3001 gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga agtcccggga
     3061 gaacccgagc cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg cggtgagcac
     3121 cggaacggca ctggtcaact tggccatggt ggccctccta tagtgagtcg tattatacta
     3181 tgccgatata ctatgccgat gattaattgt caacacgtgc tgcaggtccg aggttctagg
     3241 ctagcgagac tagcgacgag ttggctttac tccctatcag tgatagagaa cgtatgaaga
     3301 gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag
     3361 aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta
     3421 tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca
     3481 gtttactccc tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta
     3541 taaaagcaga gctcaggtag tgaaccgtca gatcgcctgg agcaattcca caacactttt
     3601 gtcttatacc aactttccgt accacttcct accctcgtaa accggtgaat tctgcagatt
     3661 gccaccatgt tgcgcttgct gttcacgctc gtcctgctgg ccctccacgg gccgtctgtc
     3721 aatgctagcc gcgactatgt gcatgttcgg ctattgagct accgaggcga ccccctggtc
     3781 ttcaagcaca ctttttcggg tgtgcgtcga cccttcaccg agctaggctg ggctgcgtgt
     3841 cgcgactggg acagtatgca ttgcacgccc ttctggtcta ccgatccgga gcagatgacc
     3901 gactcggtgc ggcgttacag cacagtgagc cccggcaagg aagtgacgct tcagcttcac
     3961 gggaaccaaa ccgtacagcc gtcgtttcta agctttacgt gccgcctgca gctagaaccc
     4021 gtggtggaaa atgttggcct ctacgtggcc tacgtggtca acgacggtga acgcccacag
     4081 cagtttttta caccgcaggt agacgtggta cgctttgctc tatatctaga gacgctctcc
     4141 cggatcgtgg aaccgttaga atcaggtcgc ctggcagtgg aatttgatac gcctgaccta
     4201 gctctggcgc ccgatttagt aagcagcctc ttcgtggccg gacacggcga gaccgacttt
     4261 tacatgaact ggacgctgcg tcgcagtcag acccactacc tggaggagat ggccttacag
     4321 gtggagattc taaagccccg cggcgtacgt caccgcgcta ttatccacca tccgaagcta
     4381 cagccgggcg ttggcctgtg gatagatttc tgcgtgtaca gctacaacgc gcgcctgacc
     4441 cgcggctacg tacgatacac cctgtcaccg aaagcgcgct tgcccgcaaa agcagagggt
     4501 tggctggtgt cactagacag attcatcgtg cagtacctca acacattgct gattacaatg
     4561 atggcggcga tatgggctcg cgttttgata acctacctgg tgtcgcggcg tcggatggtg
     4621 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     4681 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     4741 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     4801 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     4861 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     4921 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     4981 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     5041 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     5101 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     5161 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     5221 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     5281 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaatccag cacagtggcg
     5341 gccgcacgcg ttaagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
     5401 ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc
     5461 gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat
     5521 cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca
     5581 gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg
     5641 tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg
     5701 tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg
     5761 agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg
     5821 aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa
     5881 ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg
     5941 cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa
     6001 cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg
     6061 ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt
     6121 ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag
     6181 cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg
     6241 ccacctgccg gtaggtgtgt ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct
     6301 agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag
     6361 gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt
     6421 ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga
     6481 aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa
     6541 attctggccg tttttggctt ttttgttaga cggatccata acttcgtata atgtatgcta
     6601 tacgaagtta tgccaccatg tctagactgg acaagagcaa agtcataaac ggagctctgg
     6661 aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg
     6721 gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc
     6781 tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat
     6841 ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg
     6901 acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa
     6961 atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt
     7021 ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa
     7081 aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg
     7141 agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat
     7201 gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt
     7261 ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg
     7321 ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaaataact tcgtatagca
     7381 tacattatac gaagtatggc gcgccaattc cgcccctctc cctccccccc ccctaacgtt
     7441 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     7501 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     7561 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     7621 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     7681 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     7741 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     7801 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     7861 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     7921 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     7981 taataccatg gccaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     8041 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     8101 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     8161 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     8221 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggctcgc gcatggccga
     8281 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     8341 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     8401 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ctggggtgcc
     8461 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     8521 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     8581 cggtgcctga ttaattaagc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     8641 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     8701 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     8761 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     8821 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     8881 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     8941 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     9001 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     9061 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     9121 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     9181 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     9241 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     9301 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     9361 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     9421 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     9481 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9541 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9601 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     9661 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     9721 tgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     9781 ccccttcccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     9841 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     9901 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     9961 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
    10021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    10081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    10141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    10201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    10261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    10321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    10381 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    10441 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    10501 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    10561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    10621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    10681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
    10741 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
    10801 gcttgtatat ccattttcgg atctgatcag cacgtgatga aaaagcctga actcaccgcg
    10861 acgtgaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
    10921 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
    10981 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
    11041 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
    11101 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
    11161 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
    11221 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
    11281 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
    11341 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
    11401 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
    11461 cgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc cgccttctat
    11521 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    11581 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
    11641 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    11701 tgtggtttgt ccaaactaat caatgtatct tatcatgtct gtataccgtc gacctctagc
    11761 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    11821 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    11881 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    11941 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    12001 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    12061 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    12121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    12181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    12241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    12301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    12361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    12421 tccaagctgg gctgtgtgca ctaacccccc gttcagcccg accgctgcgc cttatccggt
    12481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    12541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    12601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    12661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    12721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    12781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    12841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    12901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    12961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    13021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    13081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    13141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    13201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    13261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    13321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    13381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    13441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    13501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    13561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    13621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    13681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttct
//
