LOCUS       sequence-355085-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CG1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     387..403
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     387..403
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     408..427
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        408..426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(601..1791)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1420..1439
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1839..1844,1845..1861,1862..1867))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1994..2582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2074..2093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtctgacgct cagtggaacg acgcgtaact cacgttaagg gattttggtc atgggtggct
       61 cgaccagata tggtctcgcc ccggagcagg tggtggccat cgctagtcat gacggtggca
      121 aacaggctct tgaaaccgtc caacgccttc taccagttct ctgtcaagcc cacggactaa
      181 ccccagcgca agttgtagcg attgctagta acaatggtgg caaacaggct cttgaaaccg
      241 tacagcgcct actgccagtt ctctgtcaag cccacggtct gactccggag caggtgagac
      301 cagctttggt ctagaggagg catgatgata tatttttatc ttgtgcaatg taacatcaga
      361 gattttgaga cacgggccag agctgccagg aaacagctat gaccatgtaa tacgactcac
      421 tataggggat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
      481 tcgacatact gttcttcccc gatcaagggt tggtttgcgc attcacagtt ctccgcaaga
      541 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat
      601 tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
      661 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
      721 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
      781 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
      841 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
      901 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
      961 cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa
     1021 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
     1081 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
     1141 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
     1201 gaaggctctc aagggcatgg gtcggcgctc tcccttatgc gactcctgca ttaggaagca
     1261 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgtaagga
     1321 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     1381 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     1441 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagagaat
     1501 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
     1561 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     1621 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     1681 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     1741 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc
     1801 ctgactgcgt tagcaattta actgtgataa actaccgcat taaagctaat cgatgataag
     1861 ctgtcaaaca tgagaattaa ttcatgttct ttcctgcgtt atcccctgat tctgtggata
     1921 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1981 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2041 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2101 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2161 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2221 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2281 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2341 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2401 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2461 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2521 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2581 aagaagatcc tttgatcttt tctacggg
//
