LOCUS       sequence-35509-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Zhou Z, Feng H, Hansen DF, Kato H, Luk E, Freedberg DI, Kay LE, Wu 
            C, Bai Y
  TITLE     NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B.
  JOURNAL   Nat Struct Mol Biol. 2008 Aug . 15(8):868-9.
  PUBMED    18641662
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2008 Aug . 15(8):868-9."
COMMENT     Sequence Label: pET-TBZ
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             611..633
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(664..683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(665..683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgcagccgg atctgctcga gttatatatg aggcaaaaca ccacctgaag caattgtagc
       61 cctgatcaaa gaatctaact catcgtcacc tctaatggcc aattgcagat gtcttggggt
      121 aatcctcttg actttaagat ccttggctgc attaccagcc aactccagca cttcagcagt
      181 caaatattcc aacacagcag tcaaataaat ggcagctttg gatcctactc ttgttcggcc
      241 ggtagcgtgc cttttcaggt aacgcttaat cctaccgaca ggaaactgca agccagccct
      301 tgctgaagaa gattgtgctt gagtagagga agagtacttg gtaacagctc tagtaccttc
      361 agagacagca tgcttagcca attcacctgg taagatcaat ctaacagcgg tttgaatttc
      421 tctagcagag atagtagact tcttgttata cgcagccaat ttagaagctt cagtagcgat
      481 tctttcaaag atatcgttaa cgaaagagtt caagatagac atggactttt gggaaatacc
      541 agtgtcaggg tgagtttgct tcaaaacttt gtaaatgtaa gaagagtatg tttcctttct
      601 catatgtata tctccttctt aaagttaaac aaaattattt ctagagggaa accgttgtgg
      661 tctccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgggcag cgttgggtcc
      721 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
      781 tgccttactg gttagcagaa tgaatcaccg atacgcgagc ga
//
