LOCUS       sequence-355093-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CG4
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..23,24..29)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             77..1267
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(429..448)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1441..1460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1442..1460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1465..1481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1465..1481)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      1889..2477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2378..2397
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgacagctt atcatcgatt agctttaatg cggtagttta tcacagttaa attgctaacg
       61 cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac
      121 cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat
      181 cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat
      241 gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt
      301 cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt
      361 cctgtggatt ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      421 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      481 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      541 cgccatctcc ttacatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      601 actgggctgc ttcctaatgc aggagtcgca taagggagag cgccgaccca tgcccttgag
      661 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
      721 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
      781 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
      841 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
      901 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
      961 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     1021 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     1081 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt
     1141 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
     1201 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     1261 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc
     1321 aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttgatcggg gaagaacagt
     1381 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatc
     1441 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1501 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc
     1561 aaagctggtc tcggtagcga ttgctagtca tgacggtggc aaacaggctc ttgaaaccgt
     1621 ccaacgcctt ctaccagttc tctgtcaagc ccacggacta accccagcgc aagttgtagc
     1681 gattgctagt aacaatggtg gcaaacaggc tcttgaaacc gtacagcgcc tactgccagt
     1741 tctctgtcaa gcccacggtc tgactccgga gcaagttgta gcaatgagac catatctggt
     1801 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     1861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2521 ggccttttgc tcacatgttc tttcctgcgt tatccacaga atcaggggat aacgcaggaa
     2581 agaacatgaa ttaattctca tgt
//
