LOCUS       sequence-355097-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CG6
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      416..1004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     905..924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1131..1136,1137..1153,1154..1159)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1207..2397
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1559..1578)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2571..2590)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2572..2590)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(join(2595..2603,1..8))
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(2595..2603,1..8))
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
       61 aatatatcat catgcctcct ctagaccaaa gctggtctcg gattgctagt catgacggtg
      121 gcaaacaggc tcttgaaacc gtccaacgcc ttctaccagt tctctgtcaa gcccacggac
      181 taaccccagc gcaagttgta gcgattgcta gtaacaatgg tggcaaacag gctcttgaaa
      241 ccgtacagcg cctactgcca gttctctgtc aagcccacgg tctgactccg gagcaagttg
      301 tagcgatagc ctgagaccat atctggtcga gccacccatg accaaaatcc cttaacgtga
      361 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      541 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1081 ccacagaatc aggggataac gcaggaaaga acatgaatta attctcatgt ttgacagctt
     1141 atcatcgatt agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca
     1201 ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct
     1261 gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc
     1321 gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
     1381 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
     1441 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatt
     1501 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     1561 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     1621 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     1681 ttacatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     1741 ttcctaatgc aggagtcgca taagggagag cgccgaccca tgcccttgag agccttcaac
     1801 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     1861 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     1921 gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     1981 cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     2041 gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     2101 cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     2161 gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     2221 tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
     2281 tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     2341 cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     2401 gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     2461 tgcggagaac tgtgaatgcg caaaccaacc cttgatcggg gaagaacagt atgtcgagct
     2521 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatc ccctatagtg
     2581 agtcgtatta catggtcata gct
//
