LOCUS       sequence-355112-        8294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSMG6-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Huntzinger E, Kashima I, Fauser M, Sauliere J, Izaurralde E
  TITLE     SMG6 is the catalytic endonuclease that cleaves mRNAs containing 
            nonsense codons in metazoan.
  JOURNAL   RNA. 2008 Dec . 14(12):2609-17.
  PUBMED    18974281
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2008 
            Dec . 14(12):2609-17."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E
FEATURES             Location/Qualifiers
     source          1..8294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2530..2595
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2620..2646
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5545..5586
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5596..5613
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5636..5653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5686..5820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5710..5729)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5764..5783
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6421..7009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6501..6520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7180..7971,7972..8040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7803..7822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8041..8145)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8213..8231
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccaaca tggacgcaca aacacgacga cgtgagcgtc gcgctgagaa acaagctcaa
     2581 tggaaagctg caaacggtgg atctcctcca catatggctt acccatacga tgttccagat
     2641 tacgctcctc caagcttgaa ttctatgagt cgaaagggaa cacgagaaca aaatgatata
     2701 tcgctgataa ttgggacgct gccgaaacgc ctgcagaaca aaatcgcggc cagctgcagc
     2761 cacgcagcag cagcggcaac aacaacaaag gcgttggaga cggaaaacgc tgagaatgcg
     2821 gagcccgtgg ctacgacagc aaatgcttca aggccagtcg aaatcctgag caatgctagc
     2881 acaggaggag gcaaacccga tgcccagtca aagcaacccg ttttggatac cagcgggaaa
     2941 atggcgtggg agggcgggat tccaggcatc ctgcgcgtgg ccacctcggt ggcgggcgat
     3001 gttaacagga acggacaggg tcagggcaag aacaagagcg gaaacagtgc tcggcgcagg
     3061 ggtcgcaagg acaaggagca cgctacgcag tcgccagtga cgcgcacgcg cttgaccacc
     3121 gagcatccgg attggtttga gccgctgttg ggtctgcccc atgccaagaa tatagtccat
     3181 ctgtacgatc agctcatggt cctcaatcag aggaatctta tactgataaa ctggaagaac
     3241 ttctgctaca ttcacgacca gctgcaggac gctttcaagc aacttctttt ggagcaactt
     3301 aagttcgtgt gcgagcacaa ggttgatgtc ttcttttgga aactactctt ctacaatgtg
     3361 cggaattact tgaagcgcca gcaaacggac caggcgcaca cacatacgct ccttctcatc
     3421 gagcaagcta tcaagtttta ccgcatgctc tacgacaagc tgatggccaa gtgcgtggca
     3481 tcgtcgcgct gtgacagcgc acttaaagtg gtggcccaga ggctgctcat ctgtttgggc
     3541 gatctgacgc gctaccgggt taaccatgtc aaggccacag attacatgga ggcggccagg
     3601 tactatcaga gggcgcagga actggtacct ggcaacgggg cacctttcaa ccagctggcg
     3661 gtcatttcca tttatcacca caagcgcttc gatgcagttt attactatgt ccgtagcttg
     3721 ttgacttcga actctatcca atctgccaag gaaagcctgc tggatttgtt tgatgagatt
     3781 cggcgtaagt acgaggagac cgagatgaag caatctccca tgcactatgc agctggatcg
     3841 aagaatcaaa agtcgaagca gatgcgcaag gaggtatgga tctatccgga tgggatcaga
     3901 cgcctacatc gcaccgatga caagggcaac aaggcgaagg gcaacaaggc cactatggca
     3961 gaggtcaatc gttacgagga gatgccgccg gaggagattc ttcctcgcat tgtttccctt
     4021 tatctgtact tattaggcaa actctacacc ggcacagatg tggactcgtt atacccgttg
     4081 ctaaggaaac tgcaaatcca aataggcgtc gctctaaggc acgaaaacct gttgtcacgc
     4141 tccaaactgc tgaaaatagt agcactcaac ttgttcgtcg tcgagcacaa caaacccaaa
     4201 acgtcacgcc gcgagatgcg ttatcacagc ttcaattttg ccaactcatt ctttggactc
     4261 atgatgaaga aaacgaacca gctgctggct ggttttgtag aggattctac aaatgtccag
     4321 tgtctcccag aagaagactt tgcgactgtg aacacatacc tacagttcgt gaatgtttat
     4381 gtacgttggt tgagcatcag tttggacgtg tgggaacccg tcagatcgga agagcactct
     4441 ttcatcgatt gctgggctga gctaacaatc ctttttgagt acattgaatg tttaatgggg
     4501 aagcacaaac ttgagaaaaa cgaaatactg ttggatgagg atgtggccct tagaggtttc
     4561 actccgttgg gtcgtgaaac gctgagcatg gactatctca aaagaggatc cgaacgtctt
     4621 cagtttttcg aacgtgttcg taggattggt cagttccagg aatactacgt ccagcatcaa
     4681 gaggctctcc agcagccgtt ggaatcaagc ttcactgtcg agcacctaaa cgcagcgatg
     4741 aagaacgcat tggaaaacta tgatgctgaa agttgtggtc ttgcggcgaa cgtttcaaag
     4801 attcagtgta aagccgttga ggaattgttg tgtcgttcaa ctgattccga tgtttccgag
     4861 ctgtgcaagc tgaagaaaga gcttgaggcc aaggctaggg ttaagcagat atgcaccagc
     4921 aagttggagg acattctcaa gttcgtggac acgaaaatat acatcgaggt gcgaccgcgc
     4981 tatcttctac cggataccaa ctgctttatc gactgccttg aagactttga aaaactgtca
     5041 acggagttta aacgctacac acttattata ccactgacag tggttaagga actggatggc
     5101 ctctccaagg gcgttaagtt ggactcgtac cgcagttcga agcagacgca gcgcatccac
     5161 cactttgacg aggtgtcgtc gcgggccaaa aagtcgttag agttcatcaa gtcggccaaa
     5221 ggcaatgtga aatgtgctac cacaaagggc tcgtttgtca atgcatcggt gtttgcccta
     5281 gttgaggagg aatatttgtc caatgacgac aagattctgg caacggcaat ggctgtctcg
     5341 aaaacggcaa aaaccgaaca atgcacagat ggcaagtgtt tcattcaaac ggaattggtg
     5401 ctcattacca ccgatcgtaa tctgcgtgtg aaggctcttt ctcgcaattt ggccgtcagc
     5461 gcgttggatg agtttctgca atgggccaag gattgccgcg aatcaaccgg cggccgctcg
     5521 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     5581 tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     5641 actgtgcctt ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata
     5701 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     5761 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     5821 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     5881 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc
     5941 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     6001 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
     6061 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
     6121 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
     6181 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
     6241 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     6301 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     6361 gggacagctt caaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6781 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6841 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6901 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
     7501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     7801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8161 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     8221 tatcacgagg ccctttcgtc ttcaagaatt aattctatat tctaaaaaca caaatgatac
     8281 ttctaaaaaa aaat
//
