LOCUS       sequence-355117-        9063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delC-term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 9063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delCterm_E
FEATURES             Location/Qualifiers
     source          1..9063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2530..2595
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2620..2646
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6314..6355
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6365..6382
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6405..6422)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6455..6589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6479..6498)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6533..6552
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7190..7778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7270..7289)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7949..8740,8741..8809))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8572..8591
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8810..8914)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8982..9000
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccaaca tggacgcaca aacacgacga cgtgagcgtc gcgctgagaa acaagctcaa
     2581 tggaaagctg caaacggtgg atctcctcca catatggctt acccatacga tgttccagat
     2641 tacgctcctc caagcttgaa ttctgcagat atccagcaca gtggcggccg catgcgtgaa
     2701 gccctttttt cccaagatgg ctggggctgt cagcatgtta accaggatac taattgggaa
     2761 gttcccagtt cgccagaacc agccaataag gatgcacccg gtccaccaat gtggaagcca
     2821 agcattaaca atggtactga tctttgggag tccaatttga gaaacggagg tcagccggcc
     2881 gcacagcaag ttccaaagcc gtcgtggggt catacaccat cctctaactt aggtggaaca
     2941 tggggtgagg acgacgatgg cgccgatagt agtagtgtgt ggactggagg agctgttagc
     3001 aacgcgggat ccggagctgc agtgggagta aaccaagccg gagttaatgt cggtccaggc
     3061 ggtgttgttt cgtctggcgg acctcagtgg ggacaaggtg tcgttggcgt cggacttgga
     3121 tcaactggag gtaacgggtc aagcaatata actggatcgt ctggagtcgc aacaggtagt
     3181 agcggaaact ccagcaacgc tggtaacggt tggggagacc ctcgtgaaat acgccctttg
     3241 ggagttggtg gctccatgga tattcgaaat gttgaacatc gcggcggtaa cggttctgga
     3301 gcaacttcga gcgatccacg agacattcgc atgatcgatc cgcgtgaccc tattcgagga
     3361 gatccccgtg gaatatctgg tcgtcttaat gggacctctg aaatgtgggg tcatcatcca
     3421 caaatgtccc ataaccagtt gcaaggtatc aacaaaatgg ttggtcaaag tgtagcaact
     3481 gccagcacca gtgtcggaac atctggctcg ggcatcggtc ctggaggtcc cggtcctagt
     3541 acagtatcag gcaatatccc aacacagtgg gggcctgctc aaccggtaag cgttggtgta
     3601 agtggtccca aagacatgtc aaaacagata agtggatggg aggaaccatc accaccgcct
     3661 cagcgtcgca gtattcctaa ctacgatgat ggtacatcgt tgtggggtca gcaaactcgt
     3721 gttcccgctg caagcggtca ctggaaagac atgactgatt cgataggtcg tagtagtcat
     3781 ctcatgcgtg gccaaagcca aacgggaggt ataggaatag ccggcgttgg aaatagcaat
     3841 gttccagtgg gagccaatcc aagtaatcct ataagcagtg tagttggacc tcaagcccgg
     3901 attccatctg tgggcggcgt acaacacaaa ccagacggcg gcgctatgtg ggtgcattcc
     3961 ggcaatgtag gtggcagaaa taatgttgct gctgttacta cttggggaga tgacactcat
     4021 agcgttaatg tcggcgctcc cagcagtggc agtgtatcca gcaacaattg ggttgatgac
     4081 aagtccaact caaccttggc acaaaactct tggagcgacc cggcccctgt tggagttagt
     4141 tggggcaata agcaaagcaa accgccaagc aatagtgctt catcaggttg gagcactgct
     4201 gcgggcgtgg tggatggggt tgatctagga tctgagtgga acacgcacgg ggggattatt
     4261 ggaaaatctc agcaacaaca aaaactagcg ggacttaacg tgggaatggt gaacgtaatt
     4321 aacgcggaga tcattaagca aagcaagcaa tacaggatcc ttgtcgagaa cggctttaaa
     4381 aaggaagatg tagagcgggc attagtgatt gctaatatga acatcgaaga ggcagccgat
     4441 atgctccgtg ccaactcatc cctatcaatg gatggttggc gtcgacatga tgagtccctt
     4501 ggatcttatg ccgaccacaa tagttcaaca agcagcggtg gatttgctgg tcgttacccg
     4561 gtcaacagtg gacaaccttc aatgtccttt cctcataata accttatgaa taacatggga
     4621 ggtaccgctg ttactggagg taacaacaat acaaacatga cagctttaca ggtgcaaaag
     4681 tatttaaatc aagggcaaca tggtgtcgct gttggaccgc aagccgttgg taattcttca
     4741 gcagtatctg tcggatttgg tcagaacacg tctaacgcag cagtggcagg agcagcctct
     4801 gcaaatatag cagcaaatac aaacaaccaa ccgtctggtc agcaaattcg catgctaggc
     4861 cagcaaattc agttggccat tcatagtggt ttcatatcta gtcagatatt gactcaaccg
     4921 ctaactcaaa caacccttaa ccttttaaac caacttctta gcaatattaa gcatctccgg
     4981 gctgcacagc aatcccttac ccgcggggga aatgtcaatc caatggcagt gaatgtggct
     5041 atatctaaat acaagcagca aatccagaat ttacagaacc agataagtgc acaacaggct
     5101 gtgtatgtaa aacagcaaaa tatgcaacca acttcacaac aacaacagcc ccaacaacag
     5161 caacttcctt ctgttcatct aagtaactca ggcaacgact atttaagagg tcacgatgca
     5221 ataaataatt tgcaaagcaa cttttctgag ctcaatatta ataagccaag tggatatcaa
     5281 ggagcgtcca atcaacaatc ccgattaaat cagtggaagc ttccagtatt agataaggag
     5341 atcaactctg acagtacgga attttctcgt gccccaggtg caacgaaaca aaatttgacg
     5401 gccaacacaa gcaacataaa ctctttgggt cttcaaaacg atagtacatg gtcaactgga
     5461 cgcagtattg gtgacggttg gcctgatccc tcatctgata acgagaataa agactggtct
     5521 gttgctcagc caacttcagc agcaactgct tacactgatc tggtccaaga gtttgagcca
     5581 ggcaagccat ggaagggttc acagatcaaa agcatagaag atgatcccag cattacacca
     5641 ggaagcgttg ctagatctcc attgtctatt aattcgacgc caaaagatgc tgacatattt
     5701 gccaataccg gtaaaaattc accgactgat ttaccgccac taagtttatc gtcgtctaca
     5761 tggagtttta atccaaacca aaattatccg agtcacagtt ggtctgacaa tagtcaacaa
     5821 tgtaccgcca cttcggagct ttggacaagc ccgctaaata aatcatcgtc tcgaggtccc
     5881 ccgccaggat tgactgccaa ttcaaataaa tctgcaaata gtaatgcgtc aacgccaaca
     5941 actattaccg gaggtgcgaa tggatggtta cagcctcgaa gtggcggtgt tcaaaccaca
     6001 aacactaatt ggacaggtgg taacaccact tggggctcca gttggttgct tttgaaaaat
     6061 ctaacagcac agattgatgg tcctactttg cgtacactgt gtatgcagca tgggcccctt
     6121 gtcagctttc acccgtattt gaaccaagga attgccttat gtaaatatac tactcgtgag
     6181 gaggcgaaca aggcgcaaat ggcgttaaac aactgtgtcc tcgccaacac cacaatattt
     6241 gctgaatctc ccagcgagaa cgaggtgcaa agcattatgc agcacttata atctagaggg
     6301 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     6361 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     6421 taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa gatacattga
     6481 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     6541 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     6601 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     6661 aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc ttgagagcct
     6721 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     6781 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     6841 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     6901 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     6961 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     7021 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     7081 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     7141 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7201 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7261 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7321 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7381 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7441 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7501 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7561 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7621 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7681 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7741 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7801 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7861 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7921 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7981 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8041 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8101 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8161 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8221 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     8281 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8341 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8401 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8461 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8521 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     8581 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8641 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8701 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8761 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8821 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8881 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8941 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     9001 cctttcgtct tcaagaatta attctatatt ctaaaaacac aaatgatact tctaaaaaaa
     9061 aat
//
