LOCUS       sequence-355138-        1933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Reint G, Li Z, Labun K, Keskitalo S, Soppa I, Mamia K, Tolo E, 
            Szymanska M, Meza-Zepeda LA, Lorenz S, Cieslar-Pobuda A, Hu X, 
            Bordin DL, Staerk J, Valen E, Schmierer B, Varjosalo M, Taipale J, 
            Haapaniemi E
  TITLE     Rapid genome editing by CRISPR-Cas9-POLD3 fusion.
  JOURNAL   Elife. 2021 Dec 13;10. pii: 75415. doi: 10.7554/eLife.75415.
  PUBMED    34898428
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-12-13"; volume: "10. pii: 75415. doi"; pages: " 
            10.7554/eLife.75415"
COMMENT     Sequence Label: pDONR221 Cas9-POLD3
FEATURES             Location/Qualifiers
     source          1..1933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1197..1296)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1313..1332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1314..1332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1337..1353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1337..1353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1455..1474)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1533..1552)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cactataggg cgaattggcg gaaggccgtc aaggccgcat agcgctggag gaggtggaag
       61 cggaggagga ggaagcggag gaggaggtag cggacctaag aaaaagagga aggtgatgct
      121 aaaggacagt gggcctctgt tcaatactga ctatgacatc cttaaaagca acttgcagaa
      181 ctgcagcaaa tttagtgcta tacaatgtgc agctgccgtc cctagagctc ctgctgaatc
      241 ctcttcgtct tccaaaaagt ttgagcagtc acatcttcac atgtcaagtg agacacaagc
      301 caacaatgag ctgaccacca atggtcatgg cccacctgca tccaagcagg tttcccagca
      361 gcccaaagga attatgggaa tgtttgcctc caaagctgct gctaaaaccc aagaaaccaa
      421 caaggaaacg aaaacagagg ctaaagaagt aacaaatgca tctgcagcag gcaacaaggc
      481 accagggaaa gggaatatga tgagcaactt ttttggaaaa gctgctatga ataaatttaa
      541 agtcaatttg gactcagaac aagcagtgaa agaagaaaaa atagtggagc agcctacagt
      601 gtctgtcacg gaaccaaagc tggcaactcc tgcaggcctg aaaaaatcca gcaaaaaagc
      661 agagcctgtt aaggtgctgc agaaggaaaa aaaaaggggg aagcgagtag cattatctga
      721 tgatgagaca aaggaaactg aaaacatgag gaaaaagagg agaagaatca aacttcctga
      781 atctgatagc agtgaagatg aagtctttcc agactctcct ggggcttatg aagctgagtc
      841 accatcccca cctcctcctc cgtctccacc tcttgaacca gtgccaaaga ctgagcctga
      901 acctccttct gtcaagagct caagtggaga aaacaaaaga aaacgaaaac gcgtactaaa
      961 atctaaaact tacctggatg gggaaggctg catagtgact gaaaaagtct acgagagtga
     1021 atcctgcaca gatagtgaag aggagcttaa catgaagaca tcctcagtac acagaccccc
     1081 tgccatgact gtgaaaaaag aacccagaga ggaacgaaag ggccccaaga aagggactgc
     1141 tgctctgggc aaagccaaca gacaggtgtc cattactggc ttcttccaga ggaaagaccc
     1201 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1261 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     1321 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     1381 ctgatgttac attgcacaag ataaaataat atcatcatga acaataaaac tgtctgctta
     1441 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     1501 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     1561 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     1621 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     1681 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     1741 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     1801 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     1861 tcctgtttgt aattgtcctt ttaacagcga tcgctgggcc tcatgggcct tccgctcact
     1921 gcccgctttc cag
//
