LOCUS       sequence-35514-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins
            LM, Xiao H, Tjandra N, Wu C, Bai Y
  TITLE     Structural basis for recognition of centromere histone variant CenH3
            by the chaperone Scm3.
  JOURNAL   Nature. 2011 Apr 14. 472(7342):234-7.
  PUBMED    21412236
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Apr 14. 472(7342):234-7."
COMMENT     Sequence Label: pET-sCS4T
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             294..311
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             747..770
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(833..851)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      847..894
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca ccaccaccac cacaagaaat
       61 tattaatctc caaaattcca tttgcaaggc tagtgaaaga agttacagac gagtttacaa
      121 ctaaagatca ggatttacgt tggcagtcaa tggcgattat ggcgttacag gaagcaagcg
      181 aagcgtatct ggtaggatta ttggaacata caaacctctt ggcgctgcat gcaaaaagaa
      241 ttactataat gaagaaagac atgcaactag caagaagaat caggggacag tttctggttc
      301 cgcgtggatc catggagcgc cacaaactag cagacgaaaa catgagaaaa gtgtggtcca
      361 acatcataag caaatacgag tctattgaag aacaaggaga cctcgtagac ctgaaaactg
      421 gtgaaatagt tgaagataat gggcatatca agacgctgac ggccaacaac agcacgaaag
      481 ataaaagaac aaaatacaca agcgtgctca gagacattat cgacatcagc gacgaggaag
      541 atggtgataa aggtggtgtc aagcgtattt ctggtttgat ctacgaagaa gtcagagccg
      601 tcttgaaatc cttcttggaa tccgtcatca gggactctgt tacttacact gaacacgcca
      661 agagaaagac tgttacttct ttggatgttg tttatgcttt gaagagacaa ggtagaacct
      721 tatatggttt cggtggttaa ctcgagcacc accaccacca ccaccaccac taattgatta
      781 atacctaggc tgctaaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      841 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      901 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggc
//
