LOCUS       sequence-355140-        1498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Reint G, Li Z, Labun K, Keskitalo S, Soppa I, Mamia K, Tolo E, 
            Szymanska M, Meza-Zepeda LA, Lorenz S, Cieslar-Pobuda A, Hu X, 
            Bordin DL, Staerk J, Valen E, Schmierer B, Varjosalo M, Taipale J, 
            Haapaniemi E
  TITLE     Rapid genome editing by CRISPR-Cas9-POLD3 fusion.
  JOURNAL   Elife. 2021 Dec 13;10. pii: 75415. doi: 10.7554/eLife.75415.
  PUBMED    34898428
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-12-13"; volume: "10. pii: 75415. doi"; pages: " 
            10.7554/eLife.75415"
COMMENT     Sequence Label: pDONR221 Cas9-HMGB1
FEATURES             Location/Qualifiers
     source          1..1498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(762..861)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(878..897)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(879..897)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(902..918)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(902..918)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1020..1039)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1098..1117)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gaaggccgtc aaggccgcat ctcgagagct gggaggcgac agcgctggag gaggtggaag
       61 cggaggagga ggaagcggag gaggaggtag cggacctaag aaaaagagga aggtgatggg
      121 caaaggagat cctaagaagc cgagaggcaa aatgtcatca tatgcatttt ttgtgcaaac
      181 ttgtcgggag gagcataaga agaagcaccc agatgcttca gtcaacttct cagagttttc
      241 taagaagtgc tcagagaggt ggaagaccat gtctgctaaa gagaaaggaa aatttgaaga
      301 tatggcaaag gcggacaagg cccgttatga aagagaaatg aaaacctata tccctcccaa
      361 aggggagaca aaaaagaagt tcaaggatcc caatgcaccc aagaggcctc cttcggcctt
      421 cttcctcttc tgctctgagt atcgcccaaa aatcaaagga gaacatcctg gcctgtccat
      481 tggtgatgtt gcgaagaaac tgggagagat gtggaataac actgctgcag atgacaagca
      541 gccttatgaa aagaaggctg cgaagctgaa ggaaaaatac gaaaaggata ttgctgcata
      601 tcgagctaaa ggaaagcctg atgcagcaaa aaagggagtt gtcaaggctg aaaaaagcaa
      661 gaaaaagaag gaagaggagg aagatgagga agatgaagag gatgaggagg aggaggaaga
      721 tgaagaagat gaagatgaag aagaagatga tgatgatgaa gacccagctt tcttgtacaa
      781 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
      841 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
      901 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
      961 acaagataaa ataatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     1021 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
     1081 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     1141 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     1201 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     1261 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     1321 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     1381 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     1441 tccttttaac agcgatcgcg tatttcgtct cgctcgagct gggcctcatg ggccttcc
//
