LOCUS       sequence-355145-        1873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Reint G, Li Z, Labun K, Keskitalo S, Soppa I, Mamia K, Tolo E, 
            Szymanska M, Meza-Zepeda LA, Lorenz S, Cieslar-Pobuda A, Hu X, 
            Bordin DL, Staerk J, Valen E, Schmierer B, Varjosalo M, Taipale J, 
            Haapaniemi E
  TITLE     Rapid genome editing by CRISPR-Cas9-POLD3 fusion.
  JOURNAL   Elife. 2021 Dec 13;10. pii: 75415. doi: 10.7554/eLife.75415.
  PUBMED    34898428
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-12-13"; volume: "10. pii: 75415. doi"; pages: " 
            10.7554/eLife.75415"
COMMENT     Sequence Label: pDONR221 Cas9-RFC5
FEATURES             Location/Qualifiers
     source          1..1873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1137..1236)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1253..1272)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1254..1272)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1277..1293)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1277..1293)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1395..1414)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1473..1492)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cactataggg cgaattggcg gaaggccgtc aaggccgcat agcgctggag gaggtggaag
       61 cggaggagga ggaagcggag gaggaggtag cggacctaag aaaaagagga aggtgatgga
      121 gacctcagca ctcaagcagc aggagcagcc cgcggcgacc aagatcagga acctgccctg
      181 ggttgaaaaa taccggccac agaccctgaa tgatctcatt tctcatcagg acattctgag
      241 taccattcag aagtttatca atgaagaccg actgccacac ttgcttctct acggtccccc
      301 agggacaggc aagacatcta ccatcctagc ctgtgcgaaa cagctatata aagacaaaga
      361 atttggctcc atggtcttgg agctgaatgc ttcagatgac cgaggaatag acatcattcg
      421 aggaccgatc ctgagctttg ctagcacaag gacaatattt aagaaaggct ttaagctagt
      481 gatcttggat gaagcagacg ccatgactca ggacgcccag aatgccttga gaagagtaat
      541 tgagaaattc acagaaaata ccagattctg cctcatctgt aactatctgt caaagatcat
      601 ccctgccttg cagtcccgct gcacgaggtt tcggttcggt cccctgactc ctgaactcat
      661 ggttccccgc ctggaacatg tcgtggaaga agagaaagtt gatataagtg aagatggaat
      721 gaaagcacta gtcactcttt ccagtggaga catgcgtagg gctctgaaca ttttgcagag
      781 caccaatatg gcctttggga aggtgacaga ggagactgtc tacacctgca ccgggcaccc
      841 gctcaagtca gacattgcca acatcctgga ctggatgttg aatcaagatt tcaccacagc
      901 ctacagaaat attacagagt tgaaaactct gaaggggttg gcactgcatg atatcctgac
      961 agagatacac ttgtttgtgc atagagttga ctttccatct tcagttcgaa tacatttatt
     1021 gaccaaaatg gcagacattg agtacaggct ttctgttggc accaacgaga agatccagct
     1081 gagctccctc attgctgcat ttcaagtcac cagagacctg attgttgcag aggccgaccc
     1141 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1201 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     1261 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     1321 ctgatgttac attgcacaag ataaaataat atcatcatga acaataaaac tgtctgctta
     1381 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     1441 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     1501 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     1561 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     1621 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     1681 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     1741 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     1801 tcctgtttgt aattgtcctt ttaacagcga tcgctgggcc tcatgggcct tccgctcact
     1861 gcccgctttc cag
//
