LOCUS       sequence-35516-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins
            LM, Xiao H, Tjandra N, Wu C, Bai Y
  TITLE     Structural basis for recognition of centromere histone variant CenH3
            by the chaperone Scm3.
  JOURNAL   Nature. 2011 Apr 14. 472(7342):234-7.
  PUBMED    21412236
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Apr 14. 472(7342):234-7."
COMMENT     Sequence Label: pET-sC4T
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             231..248
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             435..458
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(521..539)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      535..582
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(706..725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     917..938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca ccaccaccac cacaagaaat
       61 tattaatctc caaaattcca tttgcaaggc tagtgaaaga agttacagac gagtttacaa
      121 ctaaagatca ggatttacgt tggcagtcaa tggcgattat ggcgttacag gaagcaagcg
      181 aagcgtatct ggtaggatta ttggaacata caaacctctt ggcgctgcat ctggttccgc
      241 gtggatccaa gcgtatttct ggtttgatct acgaagaagt cagagccgtc ttgaaatcct
      301 tcttggaatc cgtcatcagg gactctgtta cttacactga acacgccaag agaaagactg
      361 ttacttcttt ggatgttgtt tatgctttga agagacaagg tagaacctta tatggtttcg
      421 gtggttaact cgagcaccac caccaccacc accaccacta attgattaat acctaggctg
      481 ctaaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      541 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      601 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      661 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      721 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggggctc
      781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      901 tccacgttct ttaatagtgg actcttgttc caaactgg
//
