LOCUS       sequence-35518-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins
            LM, Xiao H, Tjandra N, Wu C, Bai Y
  TITLE     Structural basis for recognition of centromere histone variant CenH3
            by the chaperone Scm3.
  JOURNAL   Nature. 2011 Apr 14. 472(7342):234-7.
  PUBMED    21412236
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Apr 14. 472(7342):234-7."
COMMENT     Sequence Label: pET-sCS4T+N
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             294..311
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             780..803
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(866..884)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      880..927
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca ccaccaccac cacaagaaat
       61 tattaatctc caaaattcca tttgcaaggc tagtgaaaga agttacagac gagtttacaa
      121 ctaaagatca ggatttacgt tggcagtcaa tggcgattat ggcgttacag gaagcaagcg
      181 aagcgtatct ggtaggatta ttggaacata caaacctctt ggcgctgcat gcaaaaagaa
      241 ttactataat gaagaaagac atgcaactag caagaagaat caggggacag tttctggttc
      301 cgcgtggatc cggtggtggt cctaaactaa ccgatgatga agtcatggag cgccacaaac
      361 tagcagacga aaacatgaga aaagtgtggt ccaacatcat aagcaaatac gagtctattg
      421 aagaacaagg agacctcgta gacctgaaaa ctggtgaaat agttgaagat aatgggcata
      481 tcaagacgct gacggccaac aacagcacga aagataaaag aacaaaatac acaagcgtgc
      541 tcagagacat tatcgacatc agcgacgagg aagatggtga taaaggtggt gtcaagcgta
      601 tttctggttt gatctacgaa gaagtcagag ccgtcttgaa atccttcttg gaatccgtca
      661 tcagggactc tgttacttac actgaacacg ccaagagaaa gactgttact tctttggatg
      721 ttgtttatgc tttgaagaga caaggtagaa ccttatatgg tttcggtggt taactcgagc
      781 accaccacca ccaccaccac cactaattga ttaataccta ggctgctaaa caaagcccga
      841 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      901 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      961 ggg
//
