LOCUS       sequence-35519-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins
            LM, Xiao H, Tjandra N, Wu C, Bai Y
  TITLE     Structural basis for recognition of centromere histone variant CenH3
            by the chaperone Scm3.
  JOURNAL   Nature. 2011 Apr 14. 472(7342):234-7.
  PUBMED    21412236
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Apr 14. 472(7342):234-7."
COMMENT     Sequence Label: pET-s34T
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             216..233
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             420..443
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(506..524)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      520..567
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(691..710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcatca tcaccaccat cacctgttga
       61 tcagaaagtt acctttccaa agattggtca gagaaatcgc tcaagatttc aagaccgact
      121 tgagatttca atcttctgct atcggtgctt tgcaagaatc cgtcgaagca tacttagtct
      181 ctttgtttga agacactaat ctggctgcta ttcacctggt tccgcgtgga tccaagcgta
      241 tttctggttt gatctacgaa gaagtcagag ccgtcttgaa atccttcttg gaatccgtca
      301 tcagggactc tgttacttac actgaacacg ccaagagaaa gactgttact tctttggatg
      361 ttgtttatgc tttgaagaga caaggtagaa ccttatatgg tttcggtggt taactcgagc
      421 accaccacca ccaccaccac cactaattga ttaataccta ggctgctaaa caaagcccga
      481 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      541 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      601 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      661 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      721 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      781 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      841 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      901 gtggactctt gttccaaact g
//
