LOCUS       sequence-355192-        8674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of X. albilineansCascade type I-F1 Cascade genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Wimmer F, Mougiakos I, Englert F, Beisel CL
  TITLE     Rapid cell-free characterization of multi-subunit CRISPR effectors 
            and transposons.
  JOURNAL   Mol Cell. 2022 Feb 16. pii: S1097-2765(22)00102-2. doi: 
            10.1016/j.molcel.2022.01.026.
  PUBMED    35216669
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 Feb 16. pii: S1097-2765(22)00102-2. doi: 
            10.1016/j.molcel.2022.01.026."
COMMENT     Sequence Label: pXalb_IF1_Cascade
FEATURES             Location/Qualifiers
     source          1..8674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     80..101
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(794..1453)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1368..1387
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1454..1556)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1948..1965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2082..2627)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2162..2181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2703..2722
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2813..2847
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     promoter        2959..2993
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     RBS             3009..3020
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      7053..7124
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7140..7167
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      7684..7770
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7862..7889
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        7908..7999
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(8457..8674,1..238)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8544..8563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
       61 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
      121 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
      181 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa
      241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
      301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      481 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtcaggcatt tgagaagcac
      601 acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct
      661 atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat
      721 tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg caccaataac
      781 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
      841 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
      901 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
      961 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1021 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1081 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1141 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1201 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
     1261 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1321 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1381 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1441 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1501 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     1561 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     1621 tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg
     1681 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
     1741 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
     1801 accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg
     1861 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa
     1921 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     1981 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
     2041 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
     2101 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     2161 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     2221 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     2281 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     2341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     2401 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     2461 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     2521 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     2581 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     2641 ttaatcagat aaaatatttc taggctcatg agcccgaagt ggcgagcccg atcttcccca
     2701 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     2761 acgatgcgtc cggcgtagag gatctgctca tgtttgacag cttatcatcg atttgacagc
     2821 tagctcagtc ctaggtataa tactagtata ggttaatgtc aattcaagtt ttagagctag
     2881 gtacctagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     2941 ggtgcttttt ttgaagctct gacagctagc tcagtcctag gtataatgct agccccggga
     3001 aaagatctaa agaggagaaa ggatctatga cagaagaccc gcaacatctc acccgctccg
     3061 agcgcttccg ctcggccatc gccgcgttca tcgacgcgcg gcgcgaggcc aagctgaagg
     3121 gcaatgaggg cgatagcgac acagattcca agtacgacta cgccacctgg ctgtcggacg
     3181 cggcccgccg ggtcagccag atccaggccg tgacccatgt gctcaaggcc acacaccccg
     3241 atgcgcgcgg cagcagccta cacgtcgccc ccacccgttt gcaggcgcat acggaagtcg
     3301 gcacccacgt gctcggcacg gactatgccg aagacgtggt cggcaacgcc gcggcgctgg
     3361 acgtgttcaa actgctcaag cttgaggtcg acggtcggcg cctgctcgac tggatgcagg
     3421 acgacgacgc ggatctgcgc gtggcgctgc atgacaatgc gaaggtcgcc tcggactgga
     3481 tgggggcatt ctgcaatctg gtccgtcacg atgccctacc cagttcgcac caggccgcca
     3541 agcaagtgta ctggctagtc ggcgacgagc cgagagagga tacccactac cacctgctgc
     3601 aaccgctgtt ttccagcagt ctggcccacg cagtgcatgc ggatattcag gacgcgcgtt
     3661 tcggggaacg caacaaacag gcgcgacagg catatcgcga caagcaccca ttcgacggca
     3721 cctaccacga ctaccgcaat ctggtcgcac gcaaactggg cggcaccaag ccgcagaaca
     3781 tcagccagct caatagcgag cgcggcggca tcaactacct gctggcctcg ctgccaccgc
     3841 gctggacgca ggaagagcgt cctcgcacgt tgctcaagct ggactcggcg ctggatcgct
     3901 tcgcctattt cgatgaagtg ccgacgctga tcaagaagct ggcggatttc ctggccaccg
     3961 atcccccaaa gaacgatgca actcagaaaa aacgcgaggg catggaaaac gacttgatca
     4021 acaagctggc gctgtttgcc gccgagaccg cagtgcgttt cgagccaggc tggagccgca
     4081 acccggactg cgtattgcca ctctcgcagc aactctggct ggacgccgaa cgcgtggaac
     4141 tgccaatccg catcgatccg gagcatgcag agtgggaaca gcaagatcgc aacttcgtgg
     4201 acacttacca tctcggtgat tggcaggacg aaatcgccgg acaattcgcc acttggctca
     4261 atgaccgact acgcgccgcc gggatcaccg gcctcggtga cgaccagtac cggcattggg
     4321 ccaagcaagc catcgtcgat gcggcttggc ccgtgccgat gcgccgacgc gcaccggccg
     4381 gaggtgtgac atgagcagtt gtcccccttt cgaccacttg ctggtggtgc cgcacctgcg
     4441 cgtgcagaac gccaatgccg tttccagccc gctcacccac ggctttccct cgatcaccgc
     4501 gttcctgggc ctgatgtggg cgctggaacg caaggcgcgt atcgccggac tggatctggc
     4561 cttcaacgca gtcggcgtgg tctgccacga ccatcaagaa caggtcaccc aagggaactt
     4621 cgtcaagagc ttctgtctga cccgcaatcc ggtaaacaaa gacggtgcaa ccgcggcgat
     4681 tgttgaggaa ggccgcattc acttggaact gagcctggta ttcgcggtgc acagcatgcg
     4741 ctggctaggc gatccggcca ctcacgtcgc ggatctggcc gtggtgacca agctgctggc
     4801 cggcatgcgc gtcgctggcg gcagcgtgct gccgtccacg cacggctggc gtccacgcca
     4861 tcaagcgcag gtcatcgcct gcactggcac gccgtccgac cagcaggcgt tgttccgcaa
     4921 actacgactg cggctgttgc ccggcttcgc cctggtggca cgcgacgacc tgctcggcac
     4981 gcggctggcc gaactgcaag aacacacgcc cgaggccacc ggcctgcacg cttggctttc
     5041 gctgtcgcgc atcaactggc gtcacgtgcc ggatgcagcg gctggcaaag gcgagtggca
     5101 acacgatcgc agcggccggg gctggatcgt gcccatcccg gtgggctacg gcgcgctcgg
     5161 agccgtgcac aaggccggca gcgtggccaa tgcacgcgat gccaacaccc cgttccgctt
     5221 cgtcgagagc ctctactcgg tcggcgaatg gatcagcccg caccgcctgc aaacgccgca
     5281 tcacctgctc tggtacgccg acagccaacc cgctgccgac ctgtaccgct gccgtaacga
     5341 ttatcgcccc gccaccaccc accacttcga ttgaccacac gcacgacaag gaattccatc
     5401 atggctcacg ataccctcaa gaccgcatcc gtcctcgcct tcgagcgcaa actcgatccg
     5461 tccgacgcgc tgttcttctc cggcagttgg tccgcacgcc atacggcgcg cgattggcag
     5521 ccggtgcgca tccgcgaaaa gtcggtgcgc ggcaccattt ccaaccggct caaggacaag
     5581 agcaaggaac aatccgaccc ggccaaactc gacgccgcca tccagaaccc caacctgcaa
     5641 accgtggacg tcgccgcact cccgctggag gcggacacgc tgaaagtgca gttcacccta
     5701 cgcgtgcttg acggcactgg cgaaccttcg gcctgcaacg ataccgacta tcgcaagaaa
     5761 ctcctcggca ccgtcagtga ttacttgcag cagcatggct tcagcgagct ggcacggcgc
     5821 tacgcggcga atctcgccaa tggtcgcttc ctctggcgca accgcatcgg tgcagaacag
     5881 gtggaggtgc atgtcgcgca cctgcgcgac ggtaacccag tgacgcagtg gaccttccag
     5941 gcgcttgacc atgccctacg cacgctgtcc gcacccagcg acgcagccga gagcctgaac
     6001 gccttggccg cgaccatcgc cgacggcctg accggcaaaa cacatgtgct gctgcaagtc
     6061 accgccttcg tccgcatcgg tgccggtcag gaagtgttcc cctcgcagga actgatcctc
     6121 gaccgcggcc gcggcgacaa aagcaagacg ctctacgcgg tcggcgaagg cgacaaagcc
     6181 gtggcggcga ttcactcgca gaagatcggc aatgcactgc gcaccatcga cacgtggtat
     6241 ccgggcgtcg aggaaaacgg cgcgattgcc gtcgaaccct atggctcggt caccacgcaa
     6301 ggcaaagcgt atcgacaacc gaaggacaag caggacttct acaacctgct tgatgcgtgg
     6361 gtgatcaagg acaaagtgcc gagcgtggag caacagcatt tcgtcatcgc cacgttgatt
     6421 cgcggcggtg tgttcggcga atcgaactga ggcaaggcta tgcagcatta tctcgatctg
     6481 catcttcgcc ccgacccgga gcttgcgccc tatcaactgc ttggcgcgct gtacgcgcgc
     6541 ctgcaccggt ccctggtgac attgaacacc accaggatcg gcgtgagctt tcccggccat
     6601 gacaaccgcg taccgactct agggacgcat ctgcggctgc atggtgacga cagcaccctg
     6661 caccatctga tggcaaccac ctggctgcat ggtgttcgcg accatgtgac catcaccagc
     6721 atcggtgccg tgccatccga ggcagtgcac cgacaagtga cgcgtgtgca ggccaaaagc
     6781 agccctgaac gcctgcgtcg gcgtgccatg cgccgacatg ggataagtga ggatctcgcc
     6841 gtccaacgca ttccagattc ggcggcagaa cagttgcgac ttccgttcgt cgtactgggc
     6901 agccgcagca ccggccaaac cgcgttcccg gtcttcgtac ggcatggccc cgtacagcaa
     6961 gaacccgtac ctggcgactt cagcagttat ggactcagcc ggggagccac cgttccctgg
     7021 ttctgataac tcgagtaagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag
     7081 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     7141 gctcaccttc gggtgggcct ttctgcgttt atacctaggg agacgtcggc gctgcaggca
     7201 tgcaagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
     7261 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
     7321 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
     7381 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     7441 ctgkacctag ggagacgtcg gcgctgcagg catgcaagct tggctgtttt ggcggatgag
     7501 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     7561 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     7621 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
     7681 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     7741 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     7801 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
     7861 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt
     7921 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7981 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8041 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8101 tgaagatcag ttgggtgcag caaactatta actggcgaac tacttactct agcttcccgg
     8161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttacgc
     8461 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8521 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8581 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8641 tttacggcac ctcgacccca aaaaacttga tttg
//
