LOCUS       sequence-355200-        2151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  partial tRNA-Leugene from Rippkaea orientalis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Wimmer F, Mougiakos I, Englert F, Beisel CL
  TITLE     Rapid cell-free characterization of multi-subunit CRISPR effectors 
            and transposons.
  JOURNAL   Mol Cell. 2022 Feb 16. pii: S1097-2765(22)00102-2. doi: 
            10.1016/j.molcel.2022.01.026.
  PUBMED    35216669
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 Feb 16. pii: S1097-2765(22)00102-2. doi: 
            10.1016/j.molcel.2022.01.026."
COMMENT     Sequence Label: pRoTarget
FEATURES             Location/Qualifiers
     source          1..2151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(293..384)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(432..1170)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     1382..1398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1382..1398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(join(1652..2151,1..292))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
       61 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
      121 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
      181 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
      241 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc atactcttcc
      301 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      361 aatgtattta gaaaaataaa caaatagcta gctcactcgg tcgctacgct ccgggcgtga
      421 gactgcggcg ggcgctgcgg acacatacaa agttacccac agattccgtg gataagcagg
      481 ggactaacat gtgaggcaaa acagcagggc cgcgccggtg gcgtttttcc ataggctccg
      541 ccctcctgcc agagttcaca taaacagacg cttttccggt gcatctgtgg gagccgtgag
      601 gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga tccccaccgt
      661 ttccggcggg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt accggatacc
      721 tgttccgcct ttctccctta cgggaagtgt ggcgctttct catagctcac acactggtat
      781 ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag
      841 cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccgga aaagcacggt
      901 aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa
      961 acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg
     1021 ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct
     1081 tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga
     1141 aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgctcta gatttcagtg
     1201 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc
     1261 tcatgttagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
     1321 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgatttcac
     1381 acaggaaaca gctatgacca tgacacatgc atccaccatc gcagacttat catcactgag
     1441 cctccaccta gcctggatcc agtagcgaaa gctgcaagag aatgaaaatc cgttgaccgc
     1501 aaggtcgtga gagttcaagt ctctccgccc ccattaccat tttaagagat gctgcgagac
     1561 tttcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcatcgtgg ccggatcttg
     1621 cggcccctcg gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc
     1681 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     1741 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     1801 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     1861 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     1921 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     1981 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     2041 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     2101 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac g
//
