LOCUS       sequence-35521-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins
            LM, Xiao H, Tjandra N, Wu C, Bai Y
  TITLE     Structural basis for recognition of centromere histone variant CenH3
            by the chaperone Scm3.
  JOURNAL   Nature. 2011 Apr 14. 472(7342):234-7.
  PUBMED    21412236
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Apr 14. 472(7342):234-7."
COMMENT     Sequence Label: pET-HJURP-CBP
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             409..432
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(495..513)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      509..556
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(680..699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     890..911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca ccaccaccac cacctgctgc
       61 agaaactgcg tgcgagccgt cgtcgttttc agcgtcgtat gcagcgttta attgaaaagt
      121 ataatcagcc gtttgaagat accccggtgg tgcagatggc gaccctgacc tatgaaaccc
      181 cgcagggcct gcgtatttgg ggtggccgtc tgattaaaga acgtaatgaa ggcgaaattc
      241 aggatagcag catgaagcct gccgatcgta cagatggtag cgtgcaagca gcggcgtggg
      301 gcccagaact gccgagccat cgtaccgtgc tgtaaggatc cgaattctgt acaggccttg
      361 gcgcgcctgc aggcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
      421 caccaccacc actaattgat taatacctag gctgctaaac aaagcccgaa aggaagctga
      481 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      541 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      601 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      661 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      721 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      781 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
      841 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
      901 ttccaaactg gaacacactc acctatctcg tc
//
