LOCUS       sequence-35522-e         564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Sheen JH, Zoncu R, Kim D, Sabatini DM
  TITLE     Defective Regulation of Autophagy upon Leucine Deprivation Reveals a
            Targetable Liability of Human Melanoma Cells In Vitro and In Vivo.
  JOURNAL   Cancer Cell. 2011 May 17. 19(5):613-28.
  PUBMED    21575862
REFERENCE   2  (bases 1 to 564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2011 May 17. 19(5):613-28."
COMMENT     Sequence Label: pQCXI Puro DsRed-LC3-GFP
FEATURES             Location/Qualifiers
     source          1..564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    4..465
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(163..182)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     392..409
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 agcttatcat cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg
       61 ttttttaacc tcgactaaac acatgtaaag catgtgcacc gaggccccag atcagatccc
      121 atacaatggg gtaccttctg ggcatccttc agccccttgt tgaatacgct tgaggagagc
      181 catttgactc tttccacaac tatccaactc acaacgtggc actggggttg tgccgccttt
      241 gcaggtgtat cttatacacg tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg
      301 ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc ttccagagga
      361 actgcttcct tcacgacatt caacagacct tgcattcctt tggcgagagg ggaaagaccc
      421 ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg ccctcacatt gccaaaagac
      481 ggcaatatgg tggaaaataa catatagaca aacgcacacc ggccttattc caagcggctt
      541 cggccagtaa cgttaggggg gggg
//
