LOCUS       sequence-355231-        8386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAGO1-K690A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Boland A, Huntzinger E, Schmidt S, Izaurralde E, Weichenrieder O
  TITLE     Crystal structure of the MID-PIWI lobe of a eukaryotic Argonaute 
            protein.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10466-71. doi: 
            10.1073/pnas.1103946108. Epub 2011 Jun 6.
  PUBMED    21646546
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1103946108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-06-28- 28"; volume: "108"; issue: "26"; pages: 
            "10466-71"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmAGO1-K690A_M
FEATURES             Location/Qualifiers
     source          1..8386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2586..2651
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2682..2708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5692..5733
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5743..5760
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5783..5800)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5833..5967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5857..5876)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5911..5930
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6568..7156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6648..6667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7327..8118,8119..8187))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7950..7969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8188..8292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8360..8378
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 ccaacatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa gctcaatgga
     2641 aagctgcaaa cggtggatct cctccaagct tgaattctat gtacccatac gatgttccag
     2701 attacgctat gtccacggag cgtgagctgg ctcccggtgg gccagctcag ctccatccgc
     2761 acacgctgcc gctgactttt ccggatctcc agatgacctc caccgtgggc atcattggga
     2821 aagtctacga gtcacagtgg accccctcgc ccactcggcc tcagagtccc tctcaggcgc
     2881 agactagctt cgacacgctc acatcaccac cagcgcccgg ctcgtcggtc aatcccaccg
     2941 ccgtcaccag cccaagtgcc cagaatgtgg ccgctggtgg agcaactgtg gccggtgcag
     3001 cagcaactgc cgcccaggtg gcctccgcct tgggtgccac caccggcagc gtgacgccag
     3061 caattgccac cgcaacgcca gccacgcagc cggatatgcc tgtctttacg tgtccacgac
     3121 gcccgaatct cggacgagag ggtcgcccga ttgtgctgcg cgccaatcac ttccaggtga
     3181 caatgccgcg tggctatgtg catcactatg acatcaatat acagccggac aagtgtccgc
     3241 gaaaggtgaa ccgtgagatt atcgagacta tggtgcatgc ctatagcaag atcttcggag
     3301 tgctcaagcc ggtgttcgat ggtcgcaaca atctgtatac ccgcgatccc ctgcccattg
     3361 gcaacgagcg tctagagctg gaggttactc tacccggcga gggcaaagat cgaatctttc
     3421 gcgtaacgat caagtggcag gctcaggtct cgctcttcaa tttggaggaa gctctcgaag
     3481 gccgcacgcg gcagataccc tatgatgcca ttttggcgct cgatgtggtc atgcgccatc
     3541 tgcccagcat gacgtacacg ccagtgggac gtagcttctt cagttccccg gagggttact
     3601 accatcccct gggtggtgga cgcgaggttt ggttcggttt ccatcagagc gcaaggccct
     3661 cgcagtggaa gatgatgctc aatatcgatg tctcggccac cgctttctac aaggctcaac
     3721 cagtcattga cttcatgtgc gaggtgctgg acattcgcga catcaacgag cagcgcaaac
     3781 cgctcaccga ttcgcagcgc gtcaagttca ccaaagaaat taaaggcctg aagatcgaga
     3841 tcacccactg cggccagatg cgtcgcaagt atcgtgtgtg caacgtcact cgccgccccg
     3901 ctcagatgca atcattccca ctgcagctgg agaacggaca gaccgtagag tgcaccgtgg
     3961 ccaagtactt cctggacaag taccgcatga aattgcgcta cccgcacttg ccctgcctgc
     4021 aggttggcca agagcacaag cacacttacc tgcctctaga ggtgtgcaac attgtggccg
     4081 gacagcggtg cattaaaaag ctgaccgata tgcagacgtc gaccatgatc aaggccacag
     4141 ctcgttcagc tccggatcgt gagcgtgaga ttaacaactt ggtaaagcgc gccgacttca
     4201 acaacgattc gtatgtgcaa gagtttggcc tgaccatctc caattcgatg atggaggtac
     4261 gaggacgcgt cttgcctcct cccaagcttc agtatggggg acgtgtgtct accggcctca
     4321 ccggccagca gctgttcccg ccacagaaca aggtgagctt ggcctcgccc aaccagggtg
     4381 tatgggatat gcgaggcaag cagttcttca ctggcgtcga gatccgcatc tgggccatcg
     4441 cctgttttgc cccacagcgc acggtgcgcg aggatgcgct gcgtaatttc acccagcagc
     4501 tgcagaagat ctcaaacgat gcaggcatgc cgataattgg acagccgtgc ttctgtaagt
     4561 acgccaccgg gccggatcaa gtggaaccca tgttccgtta cctgaagatc accttccccg
     4621 gcctgcaact cgtcgtggtt gtgctgcccg gcaagactcc agtatacgcc gaggtgaagc
     4681 gtgtaggtga caccgttctg ggtatggcca cccagtgtgt gcaggccaag aacgtgaaca
     4741 agacatcgcc acagacgctc tctaatctgt gtctggcaat caacgtcaag ttgggcggca
     4801 tcaattcaat tctggtcccc tccattcggc caaaggtctt caatgagccg gtcatctttt
     4861 tgggtgccga tgtgacacac ccaccagctg gcgacaacaa gaaaccatcg attgccgccg
     4921 tcgtgggctc catggatgcc catccatcgc ggtatgccgc caccgttcgc gtacagcagc
     4981 accgacagga gatcatccag gagctgagca gcatggtgcg cgagctgttg atcatgttct
     5041 acaagtcgac gggcggctac aagccccacc gcatcatact ctatcgtgac ggagtctccg
     5101 agggacaatt cccacatgtc ctgcaacacg aattgaccgc cattcgcgag gcctgcatta
     5161 agctagagcc agagtatcgg ccgggcatca cattcattgt ggtgcagaag cgccatcaca
     5221 ctcgactctt ctgcgcggag aagaaggagc agagcggcaa atcgggcaat attcccgcag
     5281 gcaccaccgt cgatgtgggc atcacacatc ccaccgaatt tgatttctat ctatgcagcc
     5341 atcagggcat ccagggcacc agtcgcccct cgcactacca cgttctgtgg gacgacaatc
     5401 actttgactc ggacgagctg cagtgcctca cgtatcagct atgccgtacg tacgtgcgct
     5461 gcacccgatc cgtcagtata ccggcgccag cctactacgc ccatttagtg gccttccgtg
     5521 ccagatatca tctggtggag aaggagcacg attcgggcga gggttcgcac cagagcggct
     5581 gctcagagga tcgtacgcca ggtgccatgg ccagggccat cactgtgcac gcggatacca
     5641 agaaggtcat gtactttgcc taactcgagt ctagagggcc cgcggttcga aggtaagcct
     5701 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
     5761 tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc tcgctgatca
     5821 gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     5881 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     5941 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6001 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     6061 gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     6121 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     6181 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
     6241 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
     6301 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
     6361 ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt
     6421 ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct
     6481 gtccaggcag gtagatgacg accatcaggg acagcttcaa ggccagcaaa aggccaggaa
     6541 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6601 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6661 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6721 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6781 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6841 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6901 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6961 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7021 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7081 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7141 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7201 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7261 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7321 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7381 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7441 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7501 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7561 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7621 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7681 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7741 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7801 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7861 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7921 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     7981 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8041 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8101 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8161 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8221 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8281 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     8341 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgt
//
