LOCUS       sequence-355236-        8720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF1_92-1180-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8720)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5H...
FEATURES             Location/Qualifiers
     source          1..8720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3934..3943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6026..6067
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6077..6094
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6117..6134)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6167..6301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6191..6210)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6245..6264
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6902..7490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6982..7001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7661..8452,8453..8521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8284..8303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8522..8626)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8694..8712
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgaattctg cagatatcgt gaaggagctg ccgccgcatg cgtgcaagta
     2761 ttgcggcatc catgatccag ccacggtggt catgtgcaac aactgccgca aatggttttg
     2821 caacggtcgt ggaagcactt ccggttcgca catcatcaac catctggtga gggccaagca
     2881 tcgcgaggtg acgctccacg gggaaggtcc cctgggcgag acaatcctgg agtgctactc
     2941 ctgtggtgtg cgcaacgtct ttgtgctggg cttcattccg gccaaggccg attctgtggt
     3001 cgtgctgctc tgccgtcagc cgtgtgccgc ccaaaactcc cttaaagaca tgaactggga
     3061 ccaggaacag tggaagcctc taattgcaga ccgctgcttt ttggcctggc tggtaaagca
     3121 acccagcgaa cagggacagc tgcgagctcg ccaaatctca gccgctcaga tcaacaagct
     3181 ggaggagcta tggaaggaga atattgaggc cacgtttcag gatctggaga agccaggcat
     3241 tgactcggag ccagcacatg tgctactccg ctacgaggat ggctatcagt acgagaagac
     3301 ctttgggccg ctggtccgcc ttgaggccga atacgaccaa aaactgaagg agtctgccac
     3361 gcaggagaac atcgaagtac gctgggacgt cggcctcaac aaaaagacca ttgcctactt
     3421 tacgctggcg aagaccgatt cggacatgaa gctcatgcat ggcgacgagc tgcgcctgca
     3481 ttatgtgggc gagctgtaca atccgtggag cgagatcggc cacgttatca aggtgccgga
     3541 caatttcggc gatgacgtcg gcctggagct gaaatcctca acgaatgccc cggttaagtg
     3601 caccagtaac tttacggtgg acttcatctg gaagtgcacg tcatttgatc gcatgacacg
     3661 tgctctgtgc aaattcgcca tcgatcgcaa ttcagtgtcg aacttcatct actcgcgcct
     3721 gttgggccac ggtcgtgcgg attccaacga cgaggtgctg ttccgcggcc cacaacccaa
     3781 gctcttcagt gccccgcatc tgccggattt gaatcgcagc caggtgtatg ccgtgaaaca
     3841 cgcgcttcag cgtccgctct cgctaatcca agggccgcct ggcacgggca aaaccgtgac
     3901 ctcggcgacc atcgtttacc agctggtcaa gctccatggt ggcacagtgc tggtgtgcgc
     3961 tcccagcaac acggccgtgg atcagctaac tgagaagatc caccgaacaa accttaaagt
     4021 ggtgcgtgtt tgcgccaaga gccgtgaggc catcgatagc ccggtaagct tcctggcgct
     4081 gcacaaccaa atccgtaaca tggagaccaa ctcggagcta aagaagctgc agcagctgaa
     4141 agacgagacc ggcgagctga gctcagcaga cgaaaagcga taccgcaacc tgaaacgtgc
     4201 cgccgagaac caactgctgg aggctgccga cgttatctgc tgcacatgcg taggcgccgg
     4261 cgatggtcgt ctatcgcgag tcaagttcac ctcgatcctg atcgatgagt ctatgcagtc
     4321 gacggagccg gagtgcatgg tgccagtggt gctgggcgct aagcagctga tcctcgtggg
     4381 cgatcactgc cagctgggac cggttgttat gtgcaagaaa gcagctcgtg ccggcctctc
     4441 gcaaagtttg ttcgagcgcc tggtggttct gggcatccgt ccgttccggc tggaggtgca
     4501 atatcgcatg caccccgagc tgtcccagtt cccgtccaac ttcttctacg agggatcgct
     4561 gcaaaacggc gtctgcgcgg aggatcgtcg ccttaagctt gatttcccct ggccacagcc
     4621 ggagagaccg atgttctttc tggtaacaca gggacaggag gagattgccg gctccggcac
     4681 ctctttcctc aaccgcacag aggcggccaa cgtggaaaag attacgacgc gattccttaa
     4741 ggcaggcatc aagccggaac aaattggaat catcacgcct tacgaaggtc agcgcgctta
     4801 cctggtgcag tacatgcaat accagggcag cctgcactct cgtctatacc aggagatcga
     4861 gatcgccagt gtggacgcgt tccagggacg tgagaaggac atcattatca tgtcttgcgt
     4921 gcgatctaac gaacgtcaag gcatcggctt tttgaacgac ccacgtcgcc ttaatgttgc
     4981 ccttacacgg gccaagttcg ggatcatcat tgtgggcaat cccaaagtgc tcgccaagca
     5041 gcagctgtgg aaccatctgc tcaacttcta caaggaccgt aaggtgctcg tcgagggatc
     5101 tctgaataac cttaaggagt cgctaatcca cttccagaag cccaaaaagc ttgtcaacag
     5161 catgaacatt ggggcacact ttatgtccac cattattgcc gatgccaagg aagtgatggt
     5221 gccaggctcc atttacgacc gcagtggcgg ttacggccaa ggtcgccaaa tggtgggaca
     5281 gtcaatgaat ggcggacagt acggtggcag tggaggtggt ccctacggaa actcacccct
     5341 cggctacggt actcccagct ccaattccat ggtgggcttt ggcctgggca acggaggcaa
     5401 tggcgcggcc ggtggcaaca acaacttcgg aggggctgaa cccagttggg cggctgccca
     5461 cctccaccac gactccattg gctatatatc caacgagcat ggagcagcag cactgggcaa
     5521 catgccagtt ccggttggca tgttcatgaa catgagcaat attccgccgc gtttctacaa
     5581 ccagcaccag caggcgatca tggcggtcaa gcagaatcgc gccattcaac aacagacggg
     5641 taatttctct cccggtaact cgggtcctgg agtcactgga gtcggagtcg gacgaagcgc
     5701 caccccaggc ggcaataaga agaccaacaa gctgggaaaa tcgcgcgtaa cgggcggtgg
     5761 aactggcggg gcaccgctaa cacaaggaag ctcggtatgc aatgctgctc catacagtca
     5821 gcacccgatg cctttgtcgc tgcagatgac ccagcccagc ggatttgctc tgtcccagca
     5881 gccggaactt tcacaggact ttgggcaaat atcgcagatg gacggtttgc tatcccagga
     5941 tgttgccttt aacgcgtcgg gcgagcggag cttgaatcag ttctcacagc cttatcgctc
     6001 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     6061 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     6121 gactgtgcct tctaaggcct gagctcgctg atcagcctcg atcgaggatc cagacatgat
     6181 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     6241 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     6301 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     6361 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt cgaccgatgc
     6421 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     6481 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     6541 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     6601 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     6661 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     6721 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     6781 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     6841 agggacagct tcaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6901 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6961 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7021 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7081 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7141 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7201 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7261 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7321 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7381 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7441 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7501 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7561 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7621 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7681 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7741 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7801 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7861 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7921 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     7981 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8041 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8101 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8161 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8221 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     8281 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8341 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8401 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8461 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8521 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8581 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8641 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     8701 gtatcacgag gccctttcgt
//
