LOCUS       sequence-35524-e         607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Sheen JH, Zoncu R, Kim D, Sabatini DM
  TITLE     Defective Regulation of Autophagy upon Leucine Deprivation Reveals a
            Targetable Liability of Human Melanoma Cells In Vitro and In Vivo.
  JOURNAL   Cancer Cell. 2011 May 17. 19(5):613-28.
  PUBMED    21575862
REFERENCE   2  (bases 1 to 607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2011 May 17. 19(5):613-28."
COMMENT     Sequence Label: pQCXI Neo DsRed-LC3-GFP
FEATURES             Location/Qualifiers
     source          1..607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    41..503
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(201..220)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     430..447
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tgcgtgcaat ccatcttgtt cagccatggt tgtggcaagc ttatcatcgt gtttttcaaa
       61 ggaaaaccac gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac
      121 atgtaaagca tgtgcaccga ggccccagat cagatcccat acaatggggt accttctggg
      181 catccttcag ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta
      241 tccaactcac aacgtggcac tggggttgtg ccgcctttgc aggtgtatct tatacacgtg
      301 gcttttggcc gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg caaagggtcg
      361 ctacagacgt tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca
      421 acagaccttg cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga
      481 cagggccagg tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca
      541 tatagacaaa cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg
      601 gggaagg
//
