LOCUS       sequence-35526-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pDONR223-BRAF V600E
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagttgg caagtggaca
      121 ggaaacgcac catatccccc tgcctggatg ggtgtttttg gagaagcaca agcatataga
      181 ctaaaatcct ctgtttggaa accagcccga ttcaaggagg gttctgatgc actgcggtga
      241 atttttggca atgagcgggc cagcagctca atagaggcga gaatttgggg aaagagtggt
      301 ctctcatctc ttttcttttt gaggcactct gccattaatc tcttcatggc ttttggacag
      361 ttactccgta ccttactgag atctggagac aggtatcctc gtcccaccat aaaaattatc
      421 tggtccctgt tgttgatgtt tgaataaggt aactgtccag tcatcaattc atacagaaca
      481 attccaaatg catatacatc tgactgaaag ctgtatggat ttttatcttg cattctgatg
      541 acttctggtg ccatccacaa aatggatcca gacaactgtt caaactgatg ggacccactc
      601 catcgagatt tctctgtagc tagaccaaaa tcacctattt ttactgtgag gtcttcatga
      661 agaaatatat tattactctt gaggtctctg tggatgattg acttggcgtg taagtaatcc
      721 atgccctgtg cagtctgtcg tgcaatatct ataagtttga tcatctcaaa tttggtctca
      781 atgatatgga gatggtgata caagctggag ccctcacacc actgggtaac aatagccag
//
