LOCUS       sequence-35528-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway donor vector containing KRAS G12V..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pDONR223-K-RAS V12
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(101..133,134..136,137..667)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(669..768)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(785..804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(786..804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(809..825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(809..825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agttggcacc atgactgaat ataaacttgt
      121 ggtagttgga gctgttggcg taggcaagag tgccttgacg atacagctaa ttcagaatca
      181 ttttgtggac gaatatgatc caacaataga ggattcctac aggaagcaag tagtaattga
      241 tggagaaacc tgtctcttgg atattctcga cacagcaggt caagaggagt acagtgcaat
      301 gagggaccag tacatgagga ctggggaggg ctttctttgt gtatttgcca taaataatac
      361 taaatcattt gaagatattc accattatag agaacaaatt aaaagagtta aggactctga
      421 agatgtacct atggtcctag taggaaataa atgtgatttg ccttccagaa cagtagacac
      481 aaaacaggct caggacttag caagaagtta tggaattcct tttattgaaa catcagcaaa
      541 gacaagacag ggtgttgatg atgccttcta tacattagtt cgagaaattc gaaaacataa
      601 agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag tcaaagacaa agtgtgtaat
      661 tatgtaattg ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
      721 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
      781 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
      841 gtctcaaatc tctgatg
//
