LOCUS       sequence-355281-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CG7
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     320..336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..336
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     341..360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        341..359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(534..1724)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1353..1372
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1772..1777,1778..1794,1795..1800))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1927..2515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2007..2026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accagatatg gtctcgagcc agtcatgacg gtggcaaaca ggctcttgaa accgtccaac
       61 gccttctacc agttctctgt caagcccacg gactaacccc agcgcaagtt gtagcgattg
      121 ctagtaacaa tggtggcaaa caggctcttg aaaccgtaca gcgcctactg ccagttctct
      181 gtcaagccca cggtctgact ccggagcaag ttgtagcgat tgcaagttga gaccagcttt
      241 ggtctagagg aggcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
      301 agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact cactataggg
      361 gatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
      421 actgttcttc cccgatcaag ggttggtttg cgcattcaca gttctccgca agaattgatt
      481 ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
      541 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
      601 gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg
      661 acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc
      721 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
      781 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac
      841 gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg
      901 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
      961 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     1021 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     1081 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     1141 ctcaagggca tgggtcggcg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     1201 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgtaa ggagatggcg
     1261 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     1321 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     1381 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag aatccacagg
     1441 acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg
     1501 actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc
     1561 atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg
     1621 atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg
     1681 gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg
     1741 cgttagcaat ttaactgtga taaactaccg cattaaagct aatcgatgat aagctgtcaa
     1801 acatgagaat taattcatgt tctttcctgc gttatcccct gattctgtgg ataacgcagg
     1861 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1921 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1981 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2041 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2101 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2161 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2221 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2281 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2341 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2401 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2461 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2521 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgtaac tcacgttaag
     2581 ggattttggt catgggtggc tcg
//
