LOCUS       sequence-355282-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-CG8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(47..65)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(70..86)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(70..86)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      491..1079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     980..999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1206..1211,1212..1228,1229..1234)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1282..2472
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1634..1653)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgacttact ggggatcaag cctgattggg agaaaataaa atatccccta tagtgagtcg
       61 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
      121 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccaaagc tggtctcagg
      181 aagcaatcgc tacaacttgc tccggagtca gaccgtgggc ttgacagaga actggcagta
      241 ggcgctgtac ggtttcaaga gcctgtttgc caccattgtt actagcaatc gctacaactt
      301 gcgctggggt tagtccgtgg gcttgacaga gaactggtag aaggcgttgg acggtttcaa
      361 gagcctgttt gccaccgtca tgacttcgag accatatctg gtcgagccac ccatgaccaa
      421 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
      481 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      541 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      601 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      661 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      721 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      781 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      841 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      901 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      961 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1021 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1081 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1141 tctttcctgc gttatccaca gaatcagggg ataacgcagg aaagaacatg aattaattct
     1201 catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca gttaaattgc
     1261 taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
     1321 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
     1381 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
     1441 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
     1501 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
     1561 cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     1621 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     1681 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     1741 ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     1801 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg acccatgccc
     1861 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     1921 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     1981 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     2041 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     2101 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     2161 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     2221 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     2281 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     2341 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     2401 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     2461 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     2521 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga tcggggaaga
     2581 acagtatgtc gagctatttt
//
