LOCUS       sequence-355289-        2468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-hC9
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(385..390,391..407,408..413))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(540..1128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(620..639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1401..1417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1401..1417
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1422..1441
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1422..1440
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1615..2468,1..337))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2434..2453
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gagaatccac aggacgggtg
       61 tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc
      121 ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
      181 catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
      241 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
      301 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
      361 aatttaactg tgataaacta ccgcattaaa gctaatcgat gataagctgt caaacatgag
      421 aattaattca tgttctttcc tgcgttatcc cctgattctg tggataacgc aggaaagaac
      481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      961 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1141 atcttttcta cggggtctga cgctcagtgg aacgacgcgt aactcacgtt aagggatttt
     1201 ggtcatgggt ggctcgacca gatatggtct cacgcggcca acgccggatc agggcgagat
     1261 aactgggcaa caatgctctc cagcgccggc ctgccgccgt catgggaacg agaccagctt
     1321 tggtctagag gaggcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
     1381 gagacacggg ccagagctgc caggaaacag ctatgaccat gtaatacgac tcactatagg
     1441 ggatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
     1501 tactgttctt ccccgatcaa gggttggttt gcgcattcac agttctccgc aagaattgat
     1561 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
     1621 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
     1681 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
     1741 gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
     1801 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     1861 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
     1921 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
     1981 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     2041 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     2101 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     2161 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     2221 tctcaagggc atgggtcggc gctctccctt atgcgactcc tgcattagga agcagcccag
     2281 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgta aggagatggc
     2341 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     2401 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     2461 aaccgcac
//
